A preliminary study of radioimmunoimaging by 131I-COC183B2 monoclonal antibody in patients with epithelial ovarian cancer.

Monoclonal antibody 131I-COC183B2, developed in our laboratory and proved to fit for human treatment was injected intraperitoneally or subcutaneously in 13 patients. In 8 cases with i.p. injection the disease corresponded with the image, i.e. 3 primary ovarian epithelial cancers showed positive images, 1 ovarian Krukenberg tumor was negative and the other 4 negative images included 1 uterine myoma and 3 ovarian teratomas. In the subcutaneous injection group, 4 cases had ovarian carcinoma, surgery and chemotherapy. Two negative images corresponded with the clinical status-in good health, another negative case had metastatic left supraclavicular lymph node due to ovarian mucinous adenocarcinoma. The last negative image in this group was a case of benign ovarian teratoma which was proved after surgery. The 1 positive case was waiting to be proved by a scheduled third operation. The computer scintigram calculation of T/NT was 5.35 to 13.7. The results suggest that this monoclonal antibody can be used for radioimmunoimaging for the localization of ovarian carcinoma, which is not only helpful for clinical staging and differential diagnosis but is also a good follow-up method.

     

老年心力衰竭患者外周血红细胞膜脂流动性的变化及与心功能的相关性

目的观察红细胞膜脂流动性（LFU）在老年充血性心力衰竭（CHF）中的变化及其意义。方法选择CHF患者40例,正常对照者30例,用荧光分光光度计偏振技术测定外周血红细胞LFU。结果CHF组红细胞LFU明显低于正常对照组（P<0.01）;心功能越差,LFU越低（P<0.05和P<0.01）。结论红细胞LFU在一定程度上反映了CHF程度。     

与甲壳素缝线体外复合培养大鼠成肌细胞株L6细胞的形态学

目的：探讨甲壳素作为骨骼肌组织工程支架材料的可行性。 方法：实验于2002-06／12在南方医科大学应用解剖学研究所完成。大鼠成肌细胞株L6与甲壳素医用缝合线体外复合培养。倒置相差显微镜下观察细胞的形态和排列情况。并分别在体外复合培养的第1，2，3，4，5，6天，取出细胞支架复合体，扫描电镜观察成肌细胞的形态和排列情况。 结果：①扫描电子显微镜观察显示，甲壳素医用可吸收缝线的表面比较粗糙。②倒置相差显微镜下可见大鼠L6细胞与甲壳素制备的医用可吸收缝合线间黏附良好，细胞沿缝线排列呈现串珠样，并可以见到细胞聚集现象。③扫描电子显微镜观察，接种24h后，细胞主要为圆球形，随后细胞迁移、伸展、并见突起；第3天，成肌细胞主要为梭形，并见融合趋势；4d后，细胞沿材料纵轴排列并相互融合，并见肌小管样结构形成及细胞外基质分泌。 结论：甲壳素具有作为支架以构建组织工程化骨骼肌的潜能，支架的平行排列有助于工程化骨骼肌纤维良好方向性的形成。     

卫生保健课程教学误区之我见

卫生保健课程是以贯彻＂预防为主＂的方针、实现＂健康为人人,人人为健康＂的一门重要学科。笔者根据数年的教学实践,结合学生的课堂调查结果,总结卫生保健课程教学中常见的误区,以期同行在教学中避免走进误区,更好地为教学服务。     

兔骨髓间充质干细胞Ⅱ型胶原表达的实验研究

目的探讨兔骨髓间充质干细胞表达Ⅱ型胶原的实验研究方法。方法利用β1转化生长因子腺病毒表达载体（Ad—TGFβ1）转染兔骨髓间充质干细胞（MSCs），观察转染后的Ⅱ型胶原表达。结果细胞转染后Ⅱ型胶原在免疫印记和免疫组化检测下均呈强阳性表达。结论Ad—TGFβI转染兔骨髓MSCs可使其细胞内Ⅱ型胶原表达阳性。     

低剂量辐射增强小鼠肿瘤基因-放射治疗效应的免疫学机制研究

目的 探讨低剂量辐射全身照射对小鼠Lewis肺癌移植肿瘤基因-放疗方案中的免疫增强作用机制。方法 小鼠右后肢皮下接种Lewis肺癌细胞建立荷瘤模型,基因-放疗组中小鼠肿瘤局部注射由多聚乙烯亚胺包裹的pEgr-IL18-B7.1重组质粒,分别接受由2 Gy 局部照射和0.075 Gy 全身照射组合的不同治疗方案,通过³H-TdR标记方法检测小鼠CTL和NK细胞毒活性,ELISA方法检测TNF-α和IFN-γ分泌活性,观察各治疗组对荷瘤小鼠抗肿瘤免疫的作用。结果 在pEgr-IL18-B7.1基因治疗方案中,单次大剂量辐射局部照射后加多次低剂量全身照射与常规多次大剂量辐射局部照射相比,小鼠CTL和NK细胞毒活性显著增强,TNF-α和IFN-γ分泌活性有不同程度的增高。 结论 低剂量辐射可以通过促进CTL和NK细胞毒效应,上调TNF-α和IFN-γ细胞因子表达,从而增强机体抗肿瘤免疫功能,提高肿瘤基因-放疗的抑瘤效果。     

Feasibility of treating hyperfibrinogenemia with intermittently administered batroxobin in patients with ischemic stroke/transient ischemic attack for secondary prevention.

This study evaluated the safety and efficacy of batroxobin in treating hyperfibrinogenemia for secondary stroke prevention. Patients with ischemic stroke or transient ischemic attack (TIA) were measured for plasma fibrinogen levels. Selected participants had concomitant hyperfibrinogenemia (plasma fibrinogen > or = 3.0 g/l). Patients enrolled between 1 July 2003 and 31 December 2004 were treated with batroxobin; patients enrolled between 1 January 2002 and 30 June 2003 were treated without batroxobin. Batroxobin was administered intermittently via intravenous injection at 3-monthly intervals. Patients in both groups were followed for 1 year. Any cerebrovascular events and suspected adverse events were recorded. In total, 112 ischemic stroke/TIA patients with concomitant hyperfibrinogenemia were enrolled, 52 being treated with batroxobin and 60 without batroxobin. Six patients (11.5%) with batroxobin and 16 patients (26.7%) without batroxobin had recurrent cerebral ischemic events during follow-up. Stroke/TIA recurrence in patients without batroxobin was higher than that in patients with batroxobin (P < 0.05). Two patients with batroxobin and two patients without batroxobin developed hemorrhagic stroke during follow-up. There were five deaths (9.6%) in the batroxobin group, and seven deaths (11.7%) in the nonbatroxobin group during follow-up (P > 0.05). Intermittent intravenous injection of batroxobin can efficiently reduce the risk for stroke/TIA recurrence in patients with concomitant hyperfibrinogenemia.     

三氧化二砷纳米微粒对抑制兔血管平滑肌细胞增殖作用的实验研究

目的 了解纳米微粒与普通剂型的三氧化二砷（As2O3）对抑制体外培养的兔血管平滑肌细胞（VSMC）增殖作用的差别。方法 对照组为不加药物的细胞，实验组为3μmol／L纳米微粒和普通剂型的As2O3，干预体外培养的兔VSMC细胞72h，进行四唑氮盐（MTT）染色测定细胞吸光度（A）值。用流式细胞仪检测细胞凋亡情况，提取细胞DNA，进行凝胶电泳分析，将3组的结果进行比较。结果 3μmol／L纳米剂型的As2O3，干预细胞，细胞数量随作用时间的延长逐渐减少，细胞生长明显受抑制，而普通剂型干预细胞，生长受抑制程度较纳米剂型弱。24h时，对照组、3μmol／L普通剂型组与纳米组，A值分别为0．68±0．10、0．58±0．12、0．33±0．12，48h时分别为0．79±0．11、0．48±0．14、0．28±0．11，72h时分别为0．96±0．13、0．34±0．15、0．20士0．06，差异均有统计学意义（Ⅳ值分别为10．934、15．039、15．539，P值均〈0．01）。流式细胞仪检测，纳米剂型As2O3、普通剂型As2O3干预及对照组的细胞干预48h，细胞增殖率分别为44．97％、58．54％、74．02％，早期凋亡率分别为16．89％、11．27％、11．20％，晚期凋亡率分别为26．56％、23．60％、12．46％，坏死细胞分别为11．58％、6．59％、2．32％。DNA电泳，As2O3干预细胞，可见凋亡梯形条带，部分细胞坏死，呈模糊的无间隔片状条带。纳米剂型药物作用的细胞DNA条带，梯形条带更多、更模糊。结论 As2O3，可以抑制体外培养的VSMC增殖，且纳米剂型As2O3较普通剂型的As2O3，对细胞抑制作用更强。     

岛状皮瓣修复瘢痕性睑外翻

目的探讨眼轮匝肌蒂颞部岛状皮瓣与颞浅动脉蒂反流轴型耳后岛状皮瓣在修复瘢痕性睑外翻中的应用,提高睑外翻矫正后所致皮肤缺损的美学修复效果。方法自1998年2月以来,对18例瘢痕性睑外翻,采用眼轮匝肌蒂颞部岛状皮瓣旋转180°移位修复13例,皮瓣最大面积2.7cm×3.6cm;采用颞浅动脉蒂反流轴型耳后岛状皮瓣经面部皮下隧道转移修复5例,皮瓣最大面积3.1cm×5.4cm。结果1例反流轴型耳后岛状皮瓣术后出现静脉回流障碍,经对症处理后仅皮瓣远端部分表皮坏死,Ⅱ期植皮后治愈,其余17例皮瓣完全成活。随访6个月,睑外翻矫正,皮瓣颜色、质地与眼周皮肤相近,供区瘢痕不明显。结论根据睑外翻程度,可制备颞部岛状皮瓣或耳后岛状皮瓣转移。前者适用于修复较小面积的皮肤缺损,后者适用于修复较大面积的皮肤缺损。     

灵芝菌合剂中灵芝多糖含量的测定

目的：探讨灵芝菌合剂中灵芝多糖含量的检测方法。方法：采用分光光度法，以无水葡萄糖为对照品，检测波长为490nm。结果：此方法在0～120μg范围内线性关系良好，灵芝多糖的平均回收率为99．09%。RSB为1．14％。结论：本法简便快捷，准确度高，重现性好，可作为本制剂的质控方法。