晚发型脊柱骨骺发育不良伴进行性骨关节病患者的成骨细胞增殖分化及基因表达谱改变

目的探讨晚发型脊柱骨骺发育不良伴进行性骨关节病(SEDT PA)患者WISP3基因突变所造成的软骨及周围骨组织病变和可能的发病机制。方法从SEDT PA患者及正常同龄个体的股骨头松质骨分离成骨细胞进行体外培养,对培养的成骨细胞进行细胞增殖、骨特异性碱性磷酸酶(BAP)、骨钙素、护骨素测定,利用cDNA表达芯片进行基因表达谱分析,并用免疫组织化学方法对基因芯片进行验证。比较SEDT PA患者及正常对照的成骨细胞在细胞增殖、分化及基因表达谱等方面的改变。同时采用Northern杂交检测成骨细胞的WISP3表达情况。结果与正常人成骨细胞相比,SEDT PA患者成骨细胞的增殖能力较强(0.86±0.04vs0.71±0.10),氚胸腺嘧啶(3H TDR)的掺入量明显较高(1363cpm±350cpmvs867cpm±128cpm)。SEDT PA患者的成骨细胞骨钙素的表达水平明显低于正常同龄人(3.62ng/μg蛋白±0.3ng/μg蛋白vs0.85ng/μg蛋白±0.06ng/μg蛋白),护骨素的表达明显低于正常同龄人(9.43pg/μg蛋白±0.41pg/μg蛋白vs1.98pg/μg蛋白±0.03pg/μg蛋白),而BAP的表达未发生明显改变;WISP3基因的表达极低。cDNA表达芯片分析结果发现,与正常对照比较,SEDT PA患者的成骨细胞有22个基因表达明显上调,16个基因表达明显下调,这些基因包括有成骨细胞的标记分子、细胞外基     

The effect of an application-based educational intervention with a social cognitive theory model on pregnant women in Denpasar,Bali, Indonesia: a randomized controlled trial

ObjectivesThe aim of this study was to elucidate the effect of application-based antenatal education based on social cognitive theory (SCT) on Health Promoting Lifestyle Profile II (HPLP II) scores, compliance with iron tablet consumption, and readiness for childbirth and complications among pregnant women in Denpasar, Bali, Indonesia.Methods This randomized controlled trial included 71 pregnant women in the treatment group and 74 pregnant women in the control group. The treatment group application-based antenatal education based on SCT, while the control group attended a conventional pregnancy class. Iron tablet consumption was verified by counting the remaining iron tablets. Information on participants’ lifestyles was collected using the HPLP II questionnaire with the help of an assistant. The collected data were statistically analyzed using IBM SPSS ver. 24.0.Results The antenatal education intervention effectively increased the HPLP II score by 0.32 points (2.62±0.331 before the intervention and 2.94±0.273 after). Meanwhile, the control group had a 0.13-point increase (p=0.001), from 2.67±0.336 to 2.80±0.275. There was no significant difference in iron tablet consumption (p=0.333) or readiness for delivery and complications (p=0.557) between the treatment and control groups.Conclusion Application-based antenatal education with SCT effectively increased the HPLP II scores of pregnant women in Denpasar, Bali. Although there was no significant difference in iron tablet consumption or readiness for delivery and complications, the values increased to a greater extent in the treatment group than in the control group. This education model is more suited to urban pregnant women who employed and have good internet access.     

对家兔糖尿病模型胰岛素治疗的实验观察

目的以胰岛细胞的变化和凋亡程度为判断标准,探讨家兔糖尿病模型胰岛素用药治疗的适宜方案。方法利用四氧嘧啶制作家兔糖尿病模型,设计了多次胰岛素治疗组（A组）、50R预混胰岛素治疗组（B组）、30R预混胰岛素治疗组（c组）3种治疗方案,共治疗30d,记录血糖达标时间及达标时Ins剂量,并与未治组、对照组比较血糖、胰岛素抗体及组织病理学变化;用苏木素-红染色（HE染色法）标记观察胰岛组织片及胰岛凋亡细胞。结果B组的达标胰岛素用量为（5．62±0．67）U／kg,较A、C少,C达标时胰岛素用量最多为（8．83±1．17）U／kg,差异有统计学意义（P〈0.01）。A组血糖达标时间最短为（7．00±1．31）d、C组血糖达标时间最长为（19．63±1．41）d,各组间比较差异有统计学意义（P〈0．001）。胰岛素抗体结合率C组最高,与A、B组比较差异有统计学意义（P〈0．01）。C组胰岛损害最轻,胰岛凋亡细胞最少。各治疗组胰岛受损和凋亡严重程度A〉B〉C。结论30R预混胰岛素治疗方案最佳。提供一个较高的基础胰岛素浓度加少量餐时胰岛素峰值量治疗糖尿病,血糖平稳缓降,对糖尿病胰岛细胞有治疗和保护作用。多次胰岛素3短加1中的强化治疗及太短时间达标的方法不可首选。     

凋亡蛋白抑制因子livin基因siRNA表达载体的构建与转染

目的:构建3个针对凋亡蛋白抑制因子livin基因mRNA的小干扰RNA(siRNA)表达载体,然后转染HCT-8/V细胞。为研究livin基因沉默对HCT-8/V细胞耐长春新碱特性的影响奠定基础。方法:合成3对靶基因livin的siRNA寡核苷酸序列通过退火生成转录模板,与线性pGCsi-H1/Neo/GFP质粒连接,转化DH5α钙化菌抽提重组质粒,测序鉴定。用脂质体2000将重组质粒转染HCT-8/V细胞并用G418筛选。结果:转化大肠杆菌涂布平板长出阳性菌落,测序结果与所设计、合成的livin的siRNA转录模板序列一致;在荧光显微镜下观察到HCT-8/V细胞表达绿色荧光蛋白(GFP)证实重组质粒已转染入细胞并用G418剔除了未转染质粒的细胞。结论:成功构建livin基因的siRNA表达载体,转染并筛选出转染了livin siRNA表达载体的HCT-8/V细胞。     

实行预约挂号状况分析

目的分析门诊挂号现状,提高预约挂号比例,解决老百姓＂挂号难＂的问题。方法通过现有门诊挂号的三种模式,开展调查,对统计数据进行对比分析。结果窗口挂号仍然是患者多年养成的就医习惯占99.42%;电话预约挂号占0.24%;网络预约挂号约占0.34%。结论改变患者多年养成的就医习惯,需要引导患者转变就医挂号观念,疏导患者就医挂号合理性,医院加大宣传力度,探索有效的预约挂号方法,提高预约挂号比例,减轻挂号窗口压力,缩短患者就诊时间,控制和减少院内交叉感染,真正解决＂挂号难＂的问题,提高预约挂号率。     

Study on Preparation of Low Heat High Belite Clinker from Waste Mortar and Its Modification

In order to realize low energy consumption in cement and the recycling of solid waste, the phase composition and structure of waste mortar used to prepare a high belite cement clinker, instead of some other raw materials, have here been investigated, and the belite was activated by doping with SO₃. The results show that a good high belite cement clinker can be obtained using waste mortar, limestone, coal gangue, iron powder, or iron correction raw materials combined at 1350 °C for half an hour. The addition of SO₃ greatly increased the strength of the clinker in the early phase, and overall, the ratio of calcium to silicon in belite became higher, and more Al₂O₃ entered the belite; however, the generation of C₃S was inhibited somewhat, and the content of C₄AF also increased. This study offers guidance for the application of waste mortar and the activation of belite, which offers huge environmental and economic benefits.     

慢性主动脉瓣关闭不全患者左室功能对运动的反应

本文对41例慢性主动脉瓣关闭不全(chronic aortic regurgitation,CAR)患者和20例对照者的研究指出,症状性和无症状性CAR患者在运动时呈异常左心室功能储备力,主动脉瓣返流减少,且休息时主动脉瓣关闭不全越严重,运动时返流量减少越明显。     

微创血肿穿刺术治疗高血压脑出血的疗效及其对炎性因子表达水平的影响

目的：通过对高血压脑出血患者采用微创血肿穿刺术治疗,评估其疗效及对炎性因子表达水平的影响。方法：对本院74例高血压脑出血患者依据建档顺序分2组,各37例。对照组采用小骨窗血肿清除术,研究组采用微创血肿穿刺术,术后3～6个月进行随访。对比两组手术情况、临床疗效、并发症发生率,评估术前及末次随访时两组神经功能缺损评分（NIHSS）、日常生活能力评分（BI）及检测其血清炎性因子［肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、高敏C反应蛋白（hs-CRP）］水平变化。结果：①手术情况：研究组手术用时（12.34±5.54）min,术中失血量（40.31±10.27）mL,下床活动时间（5.04±2.82）d,住院时间（16.30±2.61）d,均少于对照组,差异有统计学意义（P＜0.05）;②神经功能及日常生活能力：术前两组NIHSS评分及BI评分比较,差异无统计学意义（P＞0.05）,术后研究组NIHSS评分［（4.08±1.35）分］低于对照组［（6.49±1.13）分］,BI评分［（60.63±8.08）分］高于对照组［（46.82±6.81）分］,差异有统计学意义（P＜0.05）;③临床疗效：研究组治疗有效率91.89%（34/37）高于对照组72.97%（27/37）,差异有统计学意义（P＜0.05）;④血清炎性因子：术前两组hs-CRP、IL-6、TNF-α水平比较,差异无统计学意义（P＞0.05）,术后1周2组血清炎性因子水平均较术前降低,且研究组hs-CRP（7.14±3.05）mg/L、IL-6（14.18±3.16）ng/L、TNF-α（40.10±3.03）μg/L,低于对照组［（12.11±3.35）mg/L、IL-6（27.19±3.98）ng/L、TNF-α（53.22±3.32）μg/L］,差异有统计学意义（P＜0.05）;⑤并发症发生率：研究组并发症发生率5.41%（2/37）低于对照组24.32%（9/37）,差异有统计学意义（P＜0.05）。结论：采用微创血肿穿刺术治疗高血压脑出血效果显著,可有效改善患者神经功能及日常生活能力,减少手术用时及术中失血量,降低血清炎性因子水平,提高治疗效果,且安全性较高。     

癌因性疲劳动物模型的研究进展

癌因性疲劳(CRF)是一种与癌症本身或癌症治疗相关的常见症状,它不仅会中断患者的治疗,还会严重影响患者生存质量.目前,CRF具体的机制仍不明确,且缺乏公认的有效治疗.建立规范的相关动物模型并进一步探讨CRF的产生机制是找寻有效治疗途径的前提条件.本文综述国内外CRF的动物模型,以期找寻合适的临床前实验的造模方法,并区分...