Preparation and distribution of 5-fluorouracil 125I sodium alginate-bovine serum albumin nanoparticles

ＮＴＲＯＤＵＣＴＩＯＮＮａｎｏｐａｒｔｉｃｌｅ（ＮＰ）ｉｓａｎｃｏｌｏｉｄａｌｄｉｓｐｅｒｓｉｏｎｓｙｓｔｅｍ,ｗｉｔｈｄｉａｍｅｔｅｒｓｒａｎｇｉｎｇｆｒｏｍ１０ｎｍｔｏ１０００ｎｍ．Ｔｈｅｐａｒｔｉｃｌｅｓｅｘｉｓｔｍａｉｎｌｙｉｎｔｈｅｏｒｇａ...     

靶向化疗:基因治疗研究的新热点

１原文要点作者应用逆转录病毒感染法将目的基因Ｇ１ＣＥＡＣＤＮａ及ｐＣＤ２分别转导入高分泌ＣＥＡ的大肠癌细胞系（Ｌｏｖｏ）中,再分别接种到裸鼠皮下,成瘤后腹腔给予５ＦＣ（５００ｍｇ／ｋｇ·ｄ）治疗,疗程４０ｄ,结果显示含Ｇ１ＣＥＡＣＤＮａ及ｐＣＤ２逆...     

Survey on the knowledge of age-related macular degeneration and its risk factors among Singapore residents

Purpose:  To assess the awareness of age-related macular degeneration (AMD) and its risk factors among Singapore residents.
Methods:  A cross-sectional questionnaire-based telephone survey was conducted to ascertain the awareness of AMD with regards to knowledge of the disease entity and possible risk factors among Singapore residents. A Singapore residential telephone directory was used to identify potential households by choosing the first and last entries on randomly selected pages. Respondents included individuals from households with landline telephone connection who were willing to participate in the study after a brief introduction about the study. Verbal consent was sought before proceeding with the interview. Interpreters were used for respondents whose ability to converse in English was limited. Prior to commencement of the study, the protocol was reviewed and approved by Ethics committee of the Domain Specific Review Board.
Results:  The interviewers contacted 796 subjects from different households, of which 520 participated (response rate, 65.3%). The age of the respondents ranged from 18 to 85 (median 41) years. Only 38 (7.3%) of them were aware of AMD, the majority of whom had completed secondary or higher education. Two hundred (38.5%) and 191 (36.7%) of the respondents considered age and smoking, respectively, to be risk factors for AMD.
Conclusions:  The awareness of AMD among Singapore residents is low. AMD awareness needs to be improved by educational programmes designed for this specific purpose.     

双波长比值光谱法测定银黄口服液中绿原酸和黄芩苷含量

目的：应用双波长比值光谱法对银黄口服液中绿原酸和黄芩苷含量测定进行研究。方法：依据绿原酸和黄芩苷的比值光谱特征,选择266和342nm作为测定波长。结果：绿原酸线性范围2－20ug/ml,回收率98。9,RSD为1．58％;黄芩苷线性范围2－16ug/ml,回收率100．4％,RSD为1．32％,结论：本法为一种灵敏,准确和简便的分析方法,可在其他中药制剂中推广应用。     

乌头碱对大鼠心肌细胞钙通道阻滞作用的单通道分析

用膜片钳法,在培养的Wistar大鼠心室肌细胞上记录了L型钙通道的单通道活动。乌头碱50μg·ml^-1能显著阻断L型钙通道的活动,使其开放时间缩短、关闭时间延长、开放概率下降。与对照组比较,有显著性统计学差异(P<0.01)。而对通过L型钙通道的钡离于流幅值无明显影响。     

中药材龟板和鳖甲中DNA的提取与扩增

中药材龟板和鳖甲中ＤＮＡ的提取与扩增王亚明，周开亚，吴平，徐珞珊（南京师范大学生物系，南京２１００９７；中国药科大学生药学研究室，南京２１０００９）龟鳖类药材在我国使用历史悠久，具补阴益气的功效。《中国药典》（１９９０年版）规定龟甲（龟板）的原动物为...     

因子分析分光光度法在多组分复方制剂中的应用

因子分析分光光度法在多组分的系统中,可用来推断出系统中组分的个数及系统中各组分在各样品中的浓度。本文采用因子分析法的目标检测法对两组分的复方磺胺甲氧吡嗪片,用微机对其紫外光谱数据进行处理。结果表明,回收率分别为102.0%和102.7%,平均标准差小于2.2%。并对三组分样品(复方扑热息痛片)的模拟数据进行了处理,其结果也较理想。     

N-(4-乙氧羰基-4-取代丁酰基)-N-取代甘氨酸的合成

本文报道9个估计具有血管紧张素转化酶抑制活性的N-(4-乙氧羰基-4-取代丁酰基)-N-取代甘氨酸及其相应叔丁酯的合成和鉴定。药理初试结果,化合物Ⅶ₇和Ⅶ₉显示明显降压活性。     

硝基甘油对狗缺血区冠脉循环的作用

用实验性犬冠脉狭窄模型,冠脉内恒流灌注硝基甘油0.5μg/kg.min^-1,使冠脉血流量(CBF)增加、远端小动脉压(DCP)、冠脉血管总阻力(R_T)、小冠脉血管阻力(R_S)及冠状静脉低切血粘(η_b)减少;而主动脉压和心率无明显变化。恒流灌注硝基甘油1μg/kg.min^-1时,在开始5min内冠脉循环的变化同上,并伴大冠脉血管阻力(R_L)减少,10 min后出现CBF减少和R_T,R_{L及ηb增加。结果提示,硝基甘油有缓解和加重心肌缺血的双重作用,其作用可能与剂量张扩张远端小动脉压的程度有关。}