Status of bacterial colonization, Toll-like receptor expression and nuclear factor-kappa B activation in normal and diseased human livers

Epidemiological data on bacterial translocation (BT), colonization and inflammation in normal human livers is lacking. In this study we investigated the status of bacterial colonization and inflammation in the normal, cirrhotic primary biliary cirrhosis (PBC), and nonalcoholic steatohepatitis (NASH) human liver tissues. Comparatively normal livers showed increased bacterial colonization than PBC and NASH. We analyzed mRNA levels of Toll-like receptors (TLR) 2 and TLR4, and protein levels of TLR4. Phosphorylated IKKα (pIKKα) protein estimation served as a marker for nuclear factor-kappa B (NF-κB) activation. In spite of the increased bacterial colonization in normal liver tissues, lower levels of TLR2/4 mRNA and TLR4 and pIKKα proteins were found compared to PBC and NASH indicating the maintenance of suppressed inflammation and immune tolerance in normal livers. To our knowledge, this is the first clinical evidence showing suppressed inflammation despite bacterial colonization in normal human livers thus maintaining liver immune homeostasis.     

The hazard of pregnancy loss and stillbirth among women in Kersa,East Ethiopia: A follow up study

BackgroundAlthough pregnancy loss causes considerable challenge to women’s health, population-based studies in rural areas are not widely available in low-income countries. This study aims to determine the hazard of pregnancy loss and related factors in the rural communities of Ethiopia.MethodologyA prospective community-based study was conducted over a period of 1 year. Pregnancy was identified as early as possible by a pregnancy urine test. All pregnant women identified during the screening were followed up at their home until termination of pregnancy or delivery of the neonate. The total follow-up time was 7802 ‘pregnant person months’. A Cox regression analysis was done to estimate the hazard of pregnancy loss.ResultOut of a total of 1438 terminated pregnancies, 143 (9.9%) did not end in live birth, 116 ended due to bleeding and 27 were stillbirths. Whilst the hazard of pregnancy loss was low among women with pregnancy interval of two or more years [AHR 0.3 (95% CI: 0.15, 0.43)], it was high among women having unplanned pregnancy [AHR 2.2 (95% CI: 1.56, 3.11)], among those who complained STI like symptoms during the index pregnancy [AHR 4.5 (95% CI: 2.79, 7.38)] and among those never received antenatal care [AHR 1.8 (95% CI: 1.13, 2.73)].ConclusionPregnancy loss was higher amongst women experienced unplanned pregnancy, complained STI like symptoms and women who had not attended antenatal care services.RecommendationTo reduce pregnancy loss in rural Ethiopia expanding and promoting the use of family planning, antenatal services and other reproductive health care is necessary.     

An evaluation of the diagnostic value of clinical and radiological manifestations in patients attending the addis ababa tuberculosis centre.

Our investigation is based on 1,000 consecutive patients attending the routine outpatient services of the Addis Ababa Tuberculosis Demonstration and Training Centre (ATBDTC) in Addis Ababa during the period November 1996-March 1997. By using sputum microscopy for acid-fast bacilli (AFB), chest radiography (CXR) and clinical assessment these patients had been diagnosed as having either AFB-positive pulmonary tuberculosis (PTB) (n = 139), AFB-negative PTB (n = 61) or non-tuberculosis (non-TB) (n = 800). These three diagnostic groups were subsequently re-assessed by us with regard to selected demographic and clinical parameters, including CXR, in order to identify and weight markers significantly linked to proven PTB. The sum of the individual weights provided diagnostic scores (DS); the average DS for AFB-positive patients was 653.5 +/- 174 and that for non-TB patients was 219.1 +/- 138.7. The calculated cut-off value between these two groups was 444. Ten (7.2%) AFB-positive PTB patents had a DS below the calculated cut-off, while 46 (5.8%) of the 800 non-TB cases had diagnostic scores exceeding this value. Our DS system achieved 92.8% sensitivity and 94.3% specificity, with positive and negative predictive values of 73.7% and 98.7%, respectively. When plotting the individual DS values of the 61 AFB-negative TB patients, 24 (39.3%) fell below the cut-off. It is most likely that these patients did not have PTB. We conclude that there is a need for improved and standardized diagnostic approaches for TB-suspected patients who depend upon clinical and CXR criteria for correct diagnosis. Our score system may be helpful in this context.     

Triangulo-{ErIII3} complex showing field supported slow magnetic relaxation

The triangulo-{Er₃} complex [Er₃Cl(o-van)₃(OH)₂(H₂O)₅]Cl₃·nH₂O (n = 9.4; H(o-van) = o-vanillin) (1) was generated by an in situ method. The isolated Er(iii) complex 1 was characterized by elemental analysis and molecular spectroscopy. The results of single crystal X-ray diffraction studies have shown that 1 is built up of trinuclear [Er₃Cl(o-van)₃(OH)₂(H₂O)₅]³⁺ complex cations, chloride anions and water solvate molecules. Within the complex cation the three Er(iii) central atoms are placed at the apexes of a triangle which are bridged by three (o-van)⁻ ligands with additional chelating functions and two μ₃-OH⁻ ligands. Additionally five aqua and one chlorido ligands complete the octa-coordination of the three Er(iii) atoms. AC susceptibility measurements reveal that the compound exhibits slow magnetic relaxation with two relaxation modes.

The triangulo-{Er₃} complex [Er₃Cl(o-van)₃(OH)₂(H₂O)₅]Cl₃·nH₂O (n = 9.4; H(o-van) = o-vanillin) (1) was generated by an in situ method.     

Intestinal schistosomiasis among children in a labour village of Wonji Sugar Estate, Ethiopia

A survey for transmission of schistosomiasis was carried out in one of the labour villages of Wonji Sugar Estate, Ethiopia. Little or no schistosomiasis was present prior to establishment of this irrigation scheme. The survey revealed that 82% of the children between 5-14 years were now excreting S. mansoni eggs, and high intensities of egg excretion were observed (geometric mean intensity 377 eggs/g faeces, with 26% excreting greater than or equal to 1000 eggs/g). Large numbers of Biomphalaria pfeifferi snails were discovered in the irrigation canals near the village, of which 5% were shedding schistosome cercariae. No cases of urinary schistosomiasis were seen, although intermediate hosts of this infection were present. Poor maintenance of sanitary and water supply facilities, combined with a lack of community involvement in the control measures, were identified as major contributors to the dramatic rise in transmission. All children infected with S. mansoni were treated with praziquantel (40 mg/kg body weight). A follow up one month after chemotherapy indicated a cure rate of 69% and a reduction in geometric mean egg output of 77%.