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61.
62.
Natalie M. Gallant Kathryn E. Singh Candida Brown Virginia Kimonis Eric A. Muller II 《American journal of medical genetics. Part A》2019,179(3):498-502
ALG11‐Congenital Disorder of Glycosylation (ALG11‐CDG, also known as congenital disorder of glycosylation type Ip) is an inherited inborn error of metabolism due to abnormal protein and lipid glycosylation. We describe two unrelated patients with ALG11‐CDG due to novel mutations, review the literature of previously described affected individuals, and further expand the clinical phenotype. Both affected individuals reported here had severe psychomotor disabilities and epilepsy. Their fibroblasts synthesized truncated precursor glycan structures, consistent with ALG11‐CDG, while also showing hypoglycosylation of a novel biomarker, GP130. Surprisingly, one patient presented with normal transferrin glycosylation profile, a feature that has not been reported previously in patients with ALG11‐CDG. Together, our data expand the clinical and mutational spectrum of ALG11‐CDG. 相似文献
63.
Cystic fibrosis screening: a fetus with hyperechogenic bowel may be the index case. 总被引:1,自引:1,他引:1 下载免费PDF全文
F Muller M Dommergues B Simon-Bouy C Ferec J F Oury M C Aubry R Bessis E Vuillard E Denamur T Bienvenu J L Serre 《Journal of medical genetics》1998,35(8):657-660
BACKGROUND: The potential of hyperechogenic fetal bowel to act as a hallmark for prenatal cystic fibrosis screening in the general population is controversial. METHODS: Our goal was to evaluate the incidence of cystic fibrosis in 209 fetuses with hyperechogenic bowel diagnosed at routine ultrasonography and with no family history of cystic fibrosis. The diagnosis of cystic fibrosis was based on prenatal screening for the eight mutations most frequently observed in France (deltaF508, deltaI507, 1717-1G-->A, G542X, G551D, R553X, W1282X, N1303K) and at postnatal follow up. RESULTS: The overall incidence of cystic fibrosis was 7/209 (3.3%) which is 84 times the estimated risk of CF in the general population (112500). Of these seven cases, six were diagnosed prenatally based on DNA analysis (deltaF508/deltaF508, n=5; deltaF508/G542X, n=1). One case in which only one mutation had been recognised was diagnosed clinically after birth (deltaF508/unidentified mutation). Of the seven cases, none was diagnosed at 16-19 weeks, four at 16-24 weeks, and three after this. The incidence of heterozygous fetuses (15/209, 7%) was not significantly higher than the 5% expected in the general population. The mutations involved in these heterozygous cases were deltaF508 (n=13), G542X (n=1), and G551D (n=1). CONCLUSIONS: Screening for cystic fibrosis should be offered to families in which fetal hyperechogenic bowel is diagnosed at routine ultrasonography. This underlines the need to review genetic counselling in this situation where the fetus is the index case for a genetic disease. 相似文献
64.
BDNF increases release probability and the size of a rapidly recycling vesicle pool within rat hippocampal excitatory synapses 总被引:2,自引:1,他引:2
William J. Tyler Xiao-lei Zhang Kenichi Hartman Jochen Winterer Wolfgang Muller Patric K. Stanton Lucas Pozzo-Miller 《The Journal of physiology》2006,574(3):787-803
Exerting its actions pre-, post- and peri-synaptically, brain-derived neurotrophic factor (BDNF) is one of the most potent modulators of hippocampal synaptic function. Here, we examined the effects of BDNF on a rapidly recycling pool (RRP) of vesicles within excitatory synapses. First, we estimated vesicular release in hippocampal cultures by performing FM4-64 imaging in terminals impinging on enhanced green fluorescent protein (eGFP)-labelled dendritic spines – a hallmark of excitatory synapses. Consistent with a modulation of the RRP, BDNF increased the evoked destaining rate of FM4-64 only during the initial phase of field stimulation. Multiphoton microscopy in acute hippocampal slices confirmed these observations by selectively imaging the RRP, which was loaded with FM1-43 by hyperosmotic shock. Slices exposed to BDNF showed an increase in the evoked and spontaneous rates of FM1-43 destaining from terminals in CA1 stratum radiatum, mostly representing excitatory terminals of Schaffer collaterals. Variance-mean analysis of evoked EPSCs in CA1 pyramidal neurons further confirmed that release probability is increased in BDNF-treated slices, without changes in the number of independent release sites or average postsynaptic quantal amplitude. Because BDNF was absent during dye loading, imaging, destaining and whole-cell recordings, these results demonstrate that BDNF induces a long-lasting enhancement in the probability of transmitter release at hippocampal excitatory synapses by modulating the RRP. Since the endogenous BDNF scavenger TrkB-IgG prevented the enhancement of FM1-43 destaining rate caused by induction of long-term potentiation in acute hippocampal slices, the modulation of a rapidly recycling vesicle pool may underlie the role of BDNF in hippocampal long-term synaptic plasticity. 相似文献
65.
66.
Muller S 《International reviews of immunology》2004,23(5-6):423-436
Lymphocyte clonal restriction is caused by priming the immune system with an antigen and has been referred to infectious disease study as "original antigenic sin" (OAS), described first for influenza by Francis. OAS is a dominant feature of a normal immune response. Benefits of OAS come from the initial contact with the pathogen, which induces immunological memory. Memory is achieved by priming B and T cells of an immunologically na?ve host, and confers protection against infection with the antigen-related pathogen. Thus, a restricted antibody response to viral or parasite antigens is not per se pathogenic. However, the interplay between a "locked-in" immune response and the high genetic variation of the pathogenic agent can result in a deception of the immune system. In the following, clonal restriction of the immune response to HIV is described by giving examples of restricted anti-HIV antibody formation in maternally infected children. Clonal restriction results in host resistance of infected individuals to emerging HIV variants and quasispecies. The problems of classical approaches of vaccine design in AIDS and the lack of protection in vaccinated patients is reviewed. 相似文献
67.
Aboriginal infants at Cherbourg Aboriginal Settlement in Queensland now have growth rates which are similar to those of white Australian infants. The proportion of children under 90% of the standard weight-for-age appears to be the same as for white Australian children and meets the international standards. Apart from premature infants, no children have been found in the accepted "at risk" categories. 相似文献
68.
Firing properties of hippocampal neurons in a visually symmetrical environment: contributions of multiple sensory cues and mnemonic processes 总被引:3,自引:0,他引:3
The location-specific firing of hippocampal place cells can easily be brought under the control of experimenter-defined cues. Nevertheless, there is evidence that these firing fields are not determined just by immediate sensory input, but also by earlier states of the nervous system (O'Keefe and Speakman, 1987). Here, we report further on the roles of multiple visual cues and mnemonic processes in determining the firing of place cells. Rats were trained to chase food pellets in a cylinder with homogeneous gray walls and 1 white cue card. After a cell's field was recorded in this "standard" condition, probe sessions were conducted in which a second card was placed 180 degrees away from the first. This configuration created a diametrically symmetrical environment in which pairs of locations 180 degrees apart were identical with respect to views of the wall and cards. If place cells are strongly controlled by these immediately available views, firing in the 2-card configuration should be diametrically symmetrical. Alternatively, because the rat moves freely in the cylinder, information is available that pairs of visually identical places are not truly the same. If some mnemonic process stores and updates information about the rat's paths during the session, it is possible that the firing pattern will be different in 2 such places, especially because the original training was conducted in the 1-card, asymmetrical environment. Thirteen of 18 cells had a single, asymmetric firing pattern after the second card was introduced; the field was the same size and shape as in the 1-card configuration and in the same spatial relation to 1 of the 2 cards. The field position during 2-card sessions could be rotated 180 degrees by starting the rat by one card or the other. In further probe sessions in which the cue cards, entry location, and background cues were, in various combinations, rotated in relation to each other, these cells always showed a single field, similar in size and shape to that in the standard, and in the same relationship as in the standard to as many cues as possible. The remaining 5 cells showed complex changes over repeated 2-card sessions, and 3 of these showed paired fields, 180 degrees apart for at least some of the sessions. In one case, the second field disappeared with repeated exposures to 2 cards; in another, the second field persisted when only 1 card was used. We conclude that place cells are influenced both by the immediate sensory configuration and by internal neural states related to earlier experience in the environment. 相似文献
69.
Development of the maternal-fetal relationship was described by Rubin as an intimate "binding-in process." Recent technological advances enabled health care providers to observe, study, diagnose, and treat unborn children and led some to reconsider the relevance of the binding-in concept. Research has focused on prenatal attachment or bonding, maternal characteristics that influence prenatal attachment/bonding, and interventions to promote it. Before binding-in is dismissed as irrelevant, assessment of the prenatal attachment research, consideration of the impact of these ideas on pregnant women and their families, and the implications for maternity nurses must be examined. 相似文献
70.
Krewet E.; Verkoyen C.; Muller G.; Schell C.; Popp W.; Norpoth K. 《Carcinogenesis》1993,14(2):245-250
Investigations with [14C]benzene indicate the formation of baseadducts in vivo. Experiments to separate adducts from urineof [14C]benzene-exposed rats suggest the excretion of eightlabeled compounds different from benzene metabolites. In orderto obtain information about their structure we synthesized N7-,O6-, C8- and N2-phenylguanine. With regard to their chromatographicproperties we compared these phenylguanines with products obtainedby alkylation of guanine by metabolites of unlabeled and 14C-labeledbenzene in vivo with HPLC with UV detection and liquid scintillationcounting. Furthermore GC/MS and ELISA techniques were used todetect N7-phenylguanine. Phenylguanines could not be identifiedin collected DNA fractions. The labeled compounds detected inurine of [14C]benzene-exposed rats also showed deviations fromthe HPLC elution patterns of our reference substances. EvenN7-phenylguanine, formerly suspected to be a urinary metaboliteof benzene in the rat, could not be detected with these refinedHPLC methods. With GC/MS a compound was found in trace amountsin concentrated rat urine samples, which had a similar fragmentationpattern to N7-phenylguanine. These data could not be confirmedby a sensitive immunological assay (ELISA). No. N7-phenylguaninewas detected in purified rat urine samples. The results suggestthe excretion of a hydroxylated phenylguanine which may be formedin liver or bone marrow DNA by highly reactive hydroxylatedintermediates. The OH group might be lost because of the hightemperatures during GC/MS measurements. A hydroxy group at thephenyl-ring of N7-phenylguanine will cause other elution propertiesin HPLC compared to N7-phenylguanine. 相似文献