Antinuclear antibody screening by ELISA and IF techniques: discrepant results in juvenile idiopathic arthritis but consistency in childhood systemic lupus erythematous

Lately, many hospital laboratories are using for antinuclear antibody (ANA) screening the solid-phase immunoassays (ELISA-ANA) instead of the traditional immunofluorescence ANA test (IF-ANA). Results of previous studies that compare the two technologies show poor correlation between them in both juvenile idiopathic arthritis (JIA) and childhood lupus erythematous (SLE) patients. In this study, we investigated whether ELISA-ANA and traditional IF-ANA results are comparable in pediatric patients with different rheumatic diseases. A total of 156 consecutive patients were included in the study—90 children with JIA, 33 with reactive arthritis, 19 with SLE, 4 with idiopathic chronic uveitis, and 10 with other systemic rheumatic diseases. ANA determination was performed using both assays. The higher rate of discrepancies between the two methods of ANA screening appeared in the JIA population (19/90, p?<?0.001). All JIA patients with false-negative results by ELISA had significant or high IF-ANA titres. The prevalence of JIA-associated uveitis was higher in the group of false-negative ELISA-ANA children than in the ELISA-positive patients but without statistical significance (p?=?0.62). On the contrary, in SLE group, the consistency rate of the two assays was 100 %, and the reactivity levels of ELISA-ANA were significantly higher than in ELISA-positive JIA patients (p?<?0.001). ELISA seems to be a reliable method for screening ANA in childhood SLE, but not in JIA. Limited by the few SLE patients, our findings need further consideration.     

射频消融联合醋酸及高渗氯化钠对消融体积的影响

目的探讨射频消融（RFA）联合醋酸及高渗氯化钠对消融体积的影响。方法RFA联合50％醋酸及5％NaCl对离体牛肝进行消融。实验分5组，Ⅰ组：单纯RFA组，Ⅱ组：RFA联合纯醋酸组，Ⅲ组：RFA联合蒸馏水组，Ⅳ组：RFA联合10％氯化钠组，Ⅴ组：RFA联合50％醋酸及5％氯化钠组。结果在固定消融条件的情况下，所形成的平均消融体积长径Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ组分别为3．08、3．18、3．08、4．32和4．46cm；短径分别为2．99、2．98、2．77、4．29和4．44cm。Ⅳ组和Ⅴ组所形成的平均消融体积明显大于其他各组，消融时间较其他各组明显延长，Ⅱ组和Ⅴ组对血管旁组织有一定的消融作用。结论RFA消融联合50％醋酸及5％NaCl可显著扩大消融体积，醋酸溶液可沿组织间隙渗透，对血管旁组织进行消融。     

Effect of homologous tumor DNA on the evolution of SV40-induced hamster sarcoma

DNA extracted from SV40-induced hamster sarcoma (SV40--HS DNA) increased the survival length of animals carrying the homologous tumor and--in some cases--inhibited the development of the tumor. The efficacy of the preparation was directly proportional to the number of administrations; it did not necessarily depend on the amount of SV40--HS DNA per dose. SV40 DNA had no favourable effect on the evolution of the SV40-induced tumor, which suggests that viral DNA does not represent the active component of the SV40--HS DNA preparations. Some possible mechanisms of the effect of homologous tumor preparations are discussed.     

Possible relation between viruses and oromaxillofacial tumors. IV. Presence of the herpes antigen and anti-herpes antibodies in patients with tumors of the parotid gland

The presence of herpesvirus antigen and of antiherpesvirus antibodies was detected in 46% and in 36.3%, respectively, of the patients bearing parotid gland tumors. Very high titers of antiherpesvirus antibodies were found in 70.6% of the patients with nasopharyngeal carcinoma.     

40岁以下女性乳腺癌

我院１９８２年至１９９１年间手术治疗并经病理证实的原发性乳腺癌６５５例，分析比较４０岁以下的年青组乳癌与４０岁以上年长组乳癌在肿瘤发生部位，手术方式，淋巴结转移率，病理类型等五个方面，结果表明两组无明显差别。     

A controlled 6-month clinical trial to study the effects of a stannous fluoride dentifrice on gingivitis

OBJECTIVES: This study was conducted to assess anti-plaque and anti-gingivitis benefits of a stabilized stannous fluoride (SnF(2))/sodium hexametaphosphate (SHMP) dentifrice versus a negative control. MATERIAL AND METHODS: This was a randomized, 6-month, stratified, single-centre, double-blind, parallel group, clinical study conducted in harmony with the guidelines for evaluating chemotherapeutic products for the control of gingivitis outlined by the American Dental Association. A stabilized 0.454% SnF(2)/SHMP dentifrice was tested against a commercially available negative control dentifrice. Following baseline measurements, subjects received a dental prophylaxis. Subjects were instructed to brush twice daily for 60 s using their assigned product. Efficacy measurements were obtained at baseline, 3 and 6 months post treatment using the Modified Gingival Index, Gingival Bleeding Index and the Turesky Modified Quigley-Hein Plaque Index. Oral tissue examinations were performed at all visits. Results: A total of 140 subjects were enroled and 128 completed the study. RESULTS after 6 months showed the SnF(2) dentifrice delivered a 16.9% reduction in gingivitis (p<0.001), a 40.8% reduction (p<0.001) in gingival bleeding, and an 8.5% reduction in plaque (p=0.001) versus the negative control. Both treatments were well tolerated. CONCLUSIONS: Twice daily use of the SnF(2)/SHMP dentifrice over 6 months provided statistically significant anti-plaque and anti-gingivitis benefits relative to a negative control.     

冠状静脉逆行灌注碱性成纤维细胞生长因子的体内浓度梯度

背景：体外研究发现,碱性成纤维细胞生长因子浓度梯度能够促进干细胞的迁移和增殖。然而,采用冠状静脉逆行灌注途径能否建立在体碱性成纤维细胞生长因子浓度梯度尚不明确。目的：评价冠状静脉逆行灌注实验方法的安全性,建立并检测冠状静脉与局部缺血心肌间的碱性成纤维细胞生长因子浓度梯度,探讨逆行灌注后碱性成纤维细胞生长因子浓度梯度存在的时间窗。方法：开胸结扎法建立犬急性心肌梗死模型,1周后经冠状静脉逆行灌注碱性成纤维细胞生长因子。灌注结束后球囊充盈时间分别为0,5,10,15min。解除球囊充盈后即刻处死动物,ELISA法测量血浆和梗死区心肌、梗死边缘区心肌组织匀浆中碱性成纤维细胞生长因子的浓度,在体评价在不同时间点冠状静脉血液与梗死区心肌以及梗死边缘区心肌之间的碱性成纤维细胞生长因子浓度梯度。结果与结论：逆行灌注成功率为100％,无死亡、心脏压塞和恶性心律失常等并发症发生。灌注后5min和10min,冠状静脉血液与梗死区心肌之间碱性成纤维细胞生长因子浓度的差异有显著性意义,梗死区心肌浓度明显高于其他2种组织。球囊充盈15min后2组之间浓度差异无显著性意义。结果表明,经冠状静脉逆行灌注碱性成纤维细胞生长因子后,球囊充盈的时间为5-10min能在冠状静脉血液与梗死心肌之间建立稳定的碱性成纤维细胞生长因子浓度梯度,而且在梗死心肌区域浓度最高。此时间窗内灌注干细胞有望增强其移行活力。