兔脱细胞软骨基质颗粒复合SD大鼠脂肪干细胞对软骨内成骨的促进作用

目的:探讨兔脱细胞软骨基质颗粒(ACM)的生物相容性,阐明其对SD大鼠脂肪干细胞(ADSCs)软骨内成骨的促进作用。方法:提取原代SD大鼠ADSCs进行三向分化实验,流式细胞术检测细胞干性。采集3月龄新西兰白兔耳软骨,采用研磨浸泡法制备ACM备用。实验分为ACM组和ADSCs+ACM共培养组,在扫描电镜下观察ACM和ACM上ADSCs的超微形态表现,采用钙黄绿素/碘化丙啶(Calcein-AM/PI)荧光染色法观察ADSCs+ACM共培养组中ADSCs的生长情况。将ADSCs分为对照组和实验组(ADSCs与ACM共培养),采用CCK-8法检测2组细胞增殖活性,实时荧光定量PCR (RT-qPCR)法检测成软骨诱导7和14 d时2组细胞中Ⅱ型胶原(COL-Ⅱ)、Ⅹ型胶原(COL-Ⅹ)、SOX转录因子9 (SOX-9)、血管内皮生长因子(VEGF)、碱性磷酸酶(ALP)和Runt相关转录因子2 (RUNX-2) mRNA表达水平。结果:成功制备兔ACM,提取的SD大鼠ADSCs可以进行三向分化且表达干细胞特异性表面标志物。ADSCs可以在ACM上黏附生长。与对照组比较,实验组ADSCs增殖...     

针刺奇穴结合辨证选穴治疗脑卒中后便秘

目的 观察针刺奇穴结合辨证选穴治疗脑卒中后便秘患者的临床疗效.方法 将脑卒中后便秘患者74例随机分为观察组和对照组各37例.观察组采用针刺治疗,每日1次,连续治疗10 d;对照组采用乳果糖口服溶液治疗,每日25 mL,早餐时一次口服,连续服用10 d.比较2组患者首次排便时间(首次治疗结束后至首次排便所需要的时间)、便...     

p53和Bcl-2 mRNA表达在三七皂苷R1诱导HL-60细胞凋亡中的作用

目的：探讨三七皂苷R1诱导HL-60细胞凋亡的作用机制.方法：采用MTT比色法观察三七皂苷R1对人白血病细胞株HL-60细胞增殖的抑制作用.采用流式细胞术检测细胞凋亡改变,并以RT-PCR检测凋亡调节基因p53、Bcl-2的表达.结果：三七皂苷R1能明显抑制人白血病细胞株HL-60细胞的生长,且呈时间和浓度依赖性.三七皂苷R1作用后人白血病细胞株HL-60细胞呈现凋亡特征,流式细胞术显示凋亡细胞比例升高.RT-PCR检测可见p53 mRNA表达显著增加,而Bcl-2 mRNA表达减少.结论：三七皂苷R1能诱导人白血病细胞株HL-60细胞凋亡,其作用可能与凋亡调节基因p53的上调和Bcl-2的下调有关.     

骨肉瘤组织中VEGF和BMP-2表达及微血管密度与转移关系的研究

目的：研究骨肉瘤组织中VEGF和BMP-2的表达及微血管密度（microvessel density,MVD）与转移的关系。方法：用免疫组织化学法检测28例骨肉瘤组织中VEGF、BMP-2的表达和MVD。结果：骨肉瘤组织中VEGF的阳性信号主要位于细胞胞质,BMP阳性信号呈胞质型。VEGF、BMP-2表达和MVD与肿瘤转移密切相关（χ^2=15.731,P=0.000;χ^2=9.257,P=0.006;t=6.071,P=0.001）,VEGF表达与MVD密切有关（t=3.677,P=0.006）,VEGF的表达强度与BMP-2表达密切有关（χ^2=.257,P=0.006）。结论：在骨肉瘤的发生发展中VEGF是促进微血管生成的主要细胞因子;VEGF可能影响BMP的骨诱导过程;VEGF和BMP的表达水平与骨肉瘤的转移发生有关,检测VEGF表达BMP-2与MVD值可作为判断骨肿瘤预后的重要指标。     

轻症COVID-19患儿流行病学和临床特征分析

目的 探讨轻症2019-冠状病毒病(coronavirus disease 2019,COVID-19)患儿的流行病学和临床特征.方法 以2020年2月5日至3月10日武汉市武昌方舱医院确诊的轻症COVID-19儿童患者为研究对象,收集患儿住院期间和隔离期随访的临床、实验室、肺部影像学资料.该研究为回顾性、单中心系列病...     

床旁即时超声技术在上气道管理中的应用进展

床旁即时超声（point-of-care ultrasound, POCUS）的可视性大大提升了临床医师进行气道操作时的安全性和气道管理的精准性。POCUS成为临床医师进行快速诊断与精准治疗的必备技能之一。POCUS技术在新型冠状病毒肺炎爆发时亦发挥重要作用。文章综述了现阶段国内外POCUS技术在上气道解剖结构观察中的...     

护理病历书写质量控制措施效果评价

目的：实施护理病历书写质量控制措施,提高护理病历书写质量,体现护理记录真实、客观、准确、及时和完整性。方法：对质控措施实施前后各连续抽取的1500份护理病历,行护理病历书写缺陷项目量测评并比较。结果：实施质控措施后护理病历缺陷项目量明显减少（t=0.003）,书写质量提高。结论：质控措施是保证医院护理病历书写质量的必备要件。     

急诊护士的职业压力与应对

随着社会的发展,健康的概念已扩展为生理健康、心理健康以及良好的社会适应能力。同时随着生活节奏的加快,日益激烈的竞争,人们的心理压力也逐渐增加,更多的心理问题和身心疾病也相继出现。与健康关系密切的护理工作已经成为卫生保健行业中压力最大的职业之一。此文从护理的职业压力表现、压力源以及怎样去适应等方面进行了探讨,希望能让护士的职业压力受到社会的关注。     

Effect of the Surface Morphology of Porous Coatings on Secondary Electron Yield of Metal Surface

Surface roughening is an important material surface treatment technique, and it is particularly useful for use in secondary electron yield (SEY) suppression on metal surfaces. Porous structures produced via roughening on coatings have been confirmed to reduce SEY, but the regulation strategy and the influence of process parameters both remain unclear in the practical fabrication of effective porous structures. In this paper, the effect of the surface morphology of porous coatings on the SEY of aluminum alloy substrates was studied. Surface characterization and SEY measurements were carried out for samples with a specific process technique on their surfaces. An exponential fitting model of the correlation between surface roughness and the peak values of SEY curves, δm, was summarized. Furthermore, an implementation strategy to enable low surface SEY was achieved from the analysis of the effect of process parameters on surface morphology formation. This work will aid our understanding of the effect of the irregular surface morphology of porous coatings on SEY, thereby revealing low-cost access to the realization of an easy-to-scale process that enables low SEY.     

Microstructure of Dolostones of Different Geological Ages and Dedolomitization Reaction

Dolostone is widely distributed and commonly used as concrete aggregates. A large number of studies have shown that there are significant differences in the expansibility of different dolostones, and the key factors determining the expansibility of alkali carbonate rocks have not been clarified. In this paper, rocks were selected from five different geological ages: Jixianian, Cambrian, Ordovician, Devonian, and Triassic ages. The ordering degree and the content of MgCO₃ of dolomites in rocks of different geological ages were determined by X-ray diffraction (XRD). The degree of dedolomitization reaction in rocks cured in 80 °C, 1 mol/L NaOH solution was determined by quantitative X-ray diffraction (QXRD). The morphology of dolomites in rocks was determined by a polarizing microscope. The products of the dedolomitization reaction were determined by field emission electron microscopy (FESEM-EDS). According to the test results, the following conclusions are drawn. There is a good positive correlation between ordering degree and the molar fraction of MgCO₃ of dolomites. When the MgCO₃ mole fraction of dolomites varies from 47.17% to 49.60%, the higher the MgCO₃ mole fraction, the greater the ordering degree of dolomite. By analyzing the degree of the dedolomitization reaction of different dolostone powders cured at 80 °C in 1 mol/L NaOH solution, it is found that the older the geological age of dolostone, the slower the dedolomitization reaction rate and the lower the degree of dedolomitization reaction. The lower the ordering degree of dolomite crystal in the same geological age, the faster the rate of dedolomitization reaction and the higher the degree of dedolomitization reaction.