尿脱落细胞端粒酶活性检测在膀胱癌中的诊断价值

目的：探讨通过检测尿脱落细胞端粒酶活性早期诊断膀胱癌。方法：采用改进的银染－TRAP（端粒重复序列扩增）法,分别对45例膀胱移行细胞癌患者,10例膀胱良性病变患者及8例正常人尿脱落细胞进行了端粒酶活性检测。结果：24例G1级（分化良好）的膀胱癌患者,端粒酶活性强阳性12例,弱阳性6例（阳性率75．0％）,21例G2,G3级膀胱癌患者,强阳性16例,弱阳性3例（阳性率94．5％）,膀胱良性病变患者及正常人尿脱落细胞只有1例端粒酶活性呈弱阳性,其余全部阴性。结论：尿脱落细胞端粒酶活性检测特异性强,敏感性高,与病理分类,组织分级等无明显关系,提示有可能成为膀胱癌临床早期诊断指标之一。     

直立白薇退热抗炎作用

直立白薇水提物腹腔注射给药，对１５％酵母悬液诱发的大鼠发热有明显的退热作用，同时也有明显的抗炎作用，其醇提取物无作用。     

口腔癌联合根治术围手术期的健康教育路径

目的探讨健康教育护理临床路径在口腔癌联合根治术患者围手术期的应用效果。方法将40例口腔癌切除根治术患者随机分为对照组和实验组,每组各20例。对照组采取传统的教育方法,实验组采用临床护理路径对患者实施健康教育。结果实验组患者疾病相关知识掌握率为100%明显高于对照组的80%,两组比较,p<0.05,差异具有统计学意义。结论健康教育路径有利于保证健康教育的有效性,提高患者对疾病知识掌握和对护理工作的满意度。     

改良后巩膜加固术治疗变性近视

目的观察改良后巩膜加固术对变性近视的治疗效果。方法对有完整资料的35例(56眼)患者手术前后视力、屈光度、眼轴长度、并发症及眼底情况进行了对比观察。结果术前矫正视力0.06~1.0,术后全部患者视力均有不同程度提高,矫正视力0.08~1.2;术前眼轴长度(29.26±1.62)mm,术后均有不同程度减少,术后眼轴(28.25±1.76)mm,两者相比有显著性差异(P<0.01)。随访0.5~3年,眼轴长度为(28.52±1.96)mm与术后相比无显著性差异(P>0.05)。术前屈光度(-16.76±4.52)D),术后屈光度(-14.56±4.46)D。结论改良后巩膜加固术能阻止近视的发展,保护视功能,是目前治疗变性近视的有效方法。     

阴道镜下定点取材液基细胞学检查对早期筛查宫颈癌变的临床应用

目的:评价液基细胞学联合阴道镜定位活检对宫颈瘤变的诊断价值。方法:对637例白带增多,宫颈糜烂或性交后出血妇女分别采用TCT检查联合阴道镜活检的302例为观察组;直接阴道镜检查活检的335例为对照组。最终均以组织学诊断为金标准,对比两组组织学诊断阳性符合率。结果:观察组组织学诊断阳性符合率为74.8%(226/302);对照组为48.06%(161/335),两组诊断符合率比较,差异有高度显著性(P<0.01)。结论:阴道镜下定点取材作TCT检查能显著提高宫颈癌变的阳性检出率,是早期筛查宫颈癌变的良好方法。     

Silencing of STRN4 suppresses the malignant characteristics of cancer cells

The striatin family of proteins, comprising STRN, STRN3 and STRN4, are multidomain‐containing proteins that associate with additional proteins to form a large protein complex. We previously reported that STRN4 directly associated with protein kinases, such as MINK1, TNIK and MAP4K4, which are associated with tumor suppression or tumor progression. However, it remains unclear whether STRN4 is associated with tumor progression. In this report, we examined the role that STRN4 plays in cancer malignancy. We show that depletion of STRN4 suppresses proliferation, migration, invasion and the anchorage‐independent growth of cancer cells. In addition, STRN4 knockdown increases the sensitivity of pancreatic cancer cells to gemcitabine. Finally, we show that STRN4 knockdown suppresses the proliferation and metastasis of cancer cells in mice. Our results demonstrate a possible role of STRN4 in tumor progression.     

七氟醚吸入麻醉在小儿视网膜疾病检查和治疗中的应用

目的:探讨七氟醚吸入麻醉在小儿视网膜疾病检查和治疗中的可行性和安全性。方法:回顾分析2010年9月至2011年6月在我眼科中心采用七氟醚吸入麻醉,接受视网膜疾病检查和治疗的小儿89例,监测麻醉过程中患儿各项生命体征和血流动力学指标,观察诱导时患儿反应、术中情况和不良反应等,记录诱导时间、苏醒时间等。结果:所有患儿均能平稳进入麻醉状态,麻醉效果满意,术中生命体征稳定,血流动力学指标均在正常范围;诱导时间为(65±12)s,苏醒时间为(9.3±6.2)min;全组病例术中未见体动,麻醉诱导至术毕完全清醒均无不良反应。结论:七氟醚吸入麻醉具有诱导和苏醒迅速,术中维持平稳等优点,可广泛用于小儿视网膜疾病的检查和治疗。     

The effect of FK778 on the progression of chronic allograft nephropathy in a rat model

BACKGROUND: Chronic allograft nephropathy (CAN) still belongs to the leading causes of graft loss over the long term. The leflunomide derivative FK778 is a novel immunosuppressant with improved pharmacokinetic properties that effectively prolonged graft survival in several transplantation models. In the present study, we investigated the effects of FK778 at different phases after transplantation on the progression of CAN. METHODS: Fisher 344 kidneys were orthotopically transplanted into Lewis recipients. Recipients were treated with FK778 (5 mg/kg/day) over different time periods (early: days 0-10 only, continuous: day 0 to week 24, or late: weeks 16-24 only posttransplantation). Proteinuria was measured every 4 weeks, whereas grafts were harvested at 24 weeks posttransplantation for morphological and immunohistochemical analysis as well as transforming growth factor-beta and platelet derived growth factor-B chain expression. RESULTS: Continuous treatment with FK778 ameliorated the progression of CAN, whereas late treatment reduced proteinuria and resulted in a similar grade of CAN as compared to animals with continuous treatment. In contrast, FK778 given only during the early phase after transplantation had no effect on the progression of CAN as compared to controls. CONCLUSION: In summary, FK778 is a potent immunosuppressive drug that can delay the progression of CAN, even when given at later stages after transplantation.     

RNA干扰在哺乳动物细胞高通量基因功能研究中的应用

RNA干扰(RNAi)技术是近年来迅速发展起来的一种高效、特异、易操作的基因阻断技术,它已被应用于线虫、果蝇等低等生物的大规模功能丧失表型遗传学筛选中,并成功鉴定了大量的新基因。近期,在利用现有siRNA文库进行的小规模哺乳动物细胞功能基因筛选研究中,RNAi同样显示了卓越的性能。随着高复杂度的siRNA文库的构建和筛选策略的完善,RNAi在哺乳动物细胞高通量基因功能研究中的应用已日见成熟。     

胚鼠大脑皮质一氧化氮合酶阳性神经元的体外生长发育研究

本文在体外培养条件下观察了孕 14 d SD胚鼠大脑皮质中一氧化氮合酶阳性神经元的形态和发育过程。将孕 14 d SD胚鼠大脑皮质细胞悬液接种于 2 4孔培养板 ,实验按培养龄分 4组 ,分别在接种后 5d、10 d、15d和 2 0 d终止培养 ,继而进行NA DPH - d组织化学染色 ,观察各组一氧化氮合酶阳性神经元的数量和形态。结果显示 :培养后 5d即有一定数量的一氧化氮合酶阳性神经元出现 ,在 4个实验组中 ,以 15d组的一氧化氮合酶阳性神经元的数量为最多、突起最长 ,深染细胞数量也最多 ,大部分一氧化氮合酶阳性神经元的胞体与突起均局限分布在细胞团块内 ,胞体大小不一 ,只有少量的一氧化氮合酶阳性细胞散在分布在细胞团块之间 ,其突起伸向或围绕团块。本研究结果表明 ,在胚鼠大脑皮质的神经元内即已有一氧化氮合酶存在 ,提示一氧化氮可能与胚脑的生长发育有关