在二级医院建立延伸卒中单元模式病房的疗效分析

目的 通过在二级医院建立延伸卒中单元模式病房,探讨脑血管病新的管理模式及疗效评定.方法 将398例住院卒中患者,随机分入卒中单元病房和普通病房,通过对急性期病死率、并发症发生率、Barthel指数(BI)、NIHSS、出院时医疗满意度评分观察,评估近期疗效.通过观察卒中后两年内的复发率、病死率、NIHSS、BI、Hamiltom量表及评估远期疗效.结果 卒中单元组急性期病死率、并发症发生率低,患者满意度高,NIHSS、BI相比较,两组间有显著性差异(P＜0.05).卒中后两年内的复发率、病死率卒中单元组低于对照组.结论 延伸卒中单元的建立提高了脑血管病患者的近期及远期疗效.     

Rapid identification of small interfering RNA that can effectively inhibit the replication of multiple influenza B virus strains

Influenza B virus is a cause of substantial morbidity and mortality in humans and current vaccination strategies and antiviral drugs only provide limited protection. Here, we report the evaluation of small interfering RNA (siRNA) for repression of viral replication in cultured cells as well as in chicken embryos. Several siRNAs targeting conserved regions of the virus (in chemically synthesized or plasmid-encoded forms) were found to effectively block the replication of the influenza B virus. The siRNAs were found to offer broad protection over several strains of influenza B virus (B/Beijing/76/98, B/Beijing/37/99 and B/Jiangsu/10/03) that differ substantially in their genetic content. The antiviral effects of 500 ng siRNA-encoding plasmids or 60 nmoles synthetic siRNA were found to be comparable to that of 3.6 microg ribavirin. These results indicated that RNA interference warrants further study for management of influenza B virus infections.     

免疫介导再生障碍性贫血小鼠骨髓单个核细胞NF-κB表达变化

目的:探讨核因子κB(NF-κB)在获得性再生障碍性贫血(AA)发病机制中的作用.方法:将35只BALB/C小鼠随机分为2组,其中正常对照组15只,模型组20只.复制免疫介导AA小鼠模型,采用Q-PCR、免疫组织化学等方法检测小鼠骨髓单个核细胞NF-κB mRNA水平、蛋白表达及活性变化.结果:与正常对照组相比,AA小鼠骨髓单个核细胞NF-κB mRNA水平未见明显改变(P>0.05),NF-κB/p65蛋白表达增加,而p-κBα表达明显下降(P<0.01).结论:NF-κB蛋白表达异常及活性改变在AA的发病机制中具有一定的意义.     

建立和完善医院对外经济合同会签流程

阐述了医院管理中对外经济合同会签流程的目的和重要性。通过对某院对外经济合同会签管理的现状进行分析，总结了其中存在的缺陷和薄弱环节，结合相关法规和医院实际情况，有针对性地加以调整，最终建立并完善对外经济合同会签工作流程。     

低分子肝素的临床应用

结合文献与作者实践 ,综述了低分子肝素的临床应用。低分子肝素在防治血栓栓塞性疾病中效果良好 ,与肝素相比 ,在临床应用上有一定的优势     

《实验针灸学》实验教学现状及探索

目前各院校实验针灸学实验教学仍存在许多问题，通过分析种种原因，探讨实验教学问题改革与探索。     

支气管镜介导下氩气刀治疗肺癌的护理

氩等离子体凝固(APC)又称氩气刀,是一种利用氩等离子体束传导高频电流,无接触地热凝固组织的治疗方法。将氩等离子体用导管经纤维支气管镜导入支气管内进行治疗即称为经支气管镜氩气刀治疗。我院自2005年5月开展支气管镜介导下氩气刀治疗肺癌,取得了较好的治疗效果。现将护理配     

银染端粒重复序列扩增法检测端粒酶活性及其临床应用

目的 建立银染端粒重复序列扩增法(TRAP)测定肿瘤细胞的端粒酶活性.方法:将银染与TRAP相结合,测定了人肿瘤细胞株及穿刺细胞标本的端粒酶活性.结果:人肿瘤细胞株端粒酶活性为阳性,35例病理诊断为恶性肿瘤的穿刺标本中,33例端粒酶活性阳性,12例良性病变均为阴性.结论:银染TRAP法是特异,敏感及快速的端粒酶活性的检测方法,提示该方法可成为恶性肿瘤的诊断指标之一.     

CDK inhibitor p18(INK4c) is required for the generation of functional plasma cells

B cell terminal differentiation is associated with the onset of high-level antibody secretion and cell cycle arrest. Here the cyclin-dependent kinase (CDK) inhibitor p18(INK4c) is shown to be required within B cells for both terminating cell proliferation and differentiation of functional plasma cells. In its absence, B cells hyperproliferate in germinal centers and extrafollicular foci in response to T-dependent antigens but serum antibody titers are severely reduced, despite unimpaired germinal center formation, class switch recombination, variable region-directed hypermutation, and differentiation to antibody-containing plasmacytoid cells. The novel link between cell cycle control and plasma cell differentiation may, at least in part, relate to p18(INK4c) inhibition of CDK6. Cell cycle arrest mediated by p18(INK4C) is therefore requisite for the generation of functional plasma cells.