全文获取类型
收费全文 | 188407篇 |
免费 | 7602篇 |
国内免费 | 422篇 |
专业分类
耳鼻咽喉 | 2904篇 |
儿科学 | 5610篇 |
妇产科学 | 4593篇 |
基础医学 | 25950篇 |
口腔科学 | 6049篇 |
临床医学 | 11320篇 |
内科学 | 44215篇 |
皮肤病学 | 5767篇 |
神经病学 | 15006篇 |
特种医学 | 4215篇 |
外国民族医学 | 3篇 |
外科学 | 22864篇 |
综合类 | 922篇 |
一般理论 | 50篇 |
预防医学 | 20104篇 |
眼科学 | 4357篇 |
药学 | 13834篇 |
中国医学 | 808篇 |
肿瘤学 | 7860篇 |
出版年
2023年 | 1062篇 |
2022年 | 839篇 |
2021年 | 3400篇 |
2020年 | 1767篇 |
2019年 | 3814篇 |
2018年 | 6493篇 |
2017年 | 3884篇 |
2016年 | 3681篇 |
2015年 | 4160篇 |
2014年 | 4728篇 |
2013年 | 7340篇 |
2012年 | 12567篇 |
2011年 | 13252篇 |
2010年 | 6679篇 |
2009年 | 4759篇 |
2008年 | 10724篇 |
2007年 | 11558篇 |
2006年 | 10867篇 |
2005年 | 10675篇 |
2004年 | 9778篇 |
2003年 | 9238篇 |
2002年 | 8722篇 |
2001年 | 5900篇 |
2000年 | 6606篇 |
1999年 | 5030篇 |
1998年 | 857篇 |
1997年 | 553篇 |
1996年 | 576篇 |
1992年 | 2251篇 |
1991年 | 1978篇 |
1990年 | 1797篇 |
1989年 | 1558篇 |
1988年 | 1381篇 |
1987年 | 1376篇 |
1986年 | 1330篇 |
1985年 | 1211篇 |
1984年 | 882篇 |
1983年 | 706篇 |
1979年 | 884篇 |
1978年 | 531篇 |
1976年 | 505篇 |
1975年 | 590篇 |
1974年 | 805篇 |
1973年 | 795篇 |
1972年 | 697篇 |
1971年 | 673篇 |
1970年 | 726篇 |
1969年 | 683篇 |
1968年 | 656篇 |
1967年 | 592篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
21.
Purpose. To present a model-dependent approach for the assessment of the in vivo drug dissolution profile based on in vitrodata for the multiple unit dosage form, as an alternative to the numerical method proposed in the study by Hayashi et al, Pharm. Res. 12:1333–1337 (1995).
Methods. The data for aspirin granules administered to healthy subjects obtained in the above mentioned study were re-evaluated. The subject dissolution system was considered to consist of two subsystems connected in series, i.e. the subsystem describing the gastric-emptying process and the subsystem describing the intestinal dissolution process. The frequency response method was used to model the subject dissolution system.
Results. The model in vivodissolution profile of aspirin, assessed as the integral of the model weighting function of the subject dissolution system, was in agreement with the in vivo cumulative absorption profile calculated by the Wagner-Nelson method.
Conclusions. Comparison of dynamic properties of the subject dissolution system with the subsystem describing the gastric-emptying process yielded quantitative confirmation of the decisive role of the gastric-emptying process in the in vivodrug dissolution after administration in the multi unit dosage form. 相似文献
22.
23.
C M Kyaw C R De Araujo M R Lima E G S Gondim M M Brígido L G Giugliano 《Infection, genetics and evolution》2003,3(2):111-117
Diffusely adhering Escherichia coli (E. coli) strains (DAEC) represent a potential cause of diarrhoea in infants, and the detection of type three secretion system (TTSS) genes in DAEC would substantiate their pathogenic nature. In this work, four isolates of DAEC, recovered from stools of diarrhoeic children, were analysed by PCR, in order to detect the presence of TTSS genes. Primers targeted to the escC, escJ, escN and escV, some of the most conserved TTSS genes in enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC), were used in order to verify the occurrence of homologous genes in our DAEC isolates. By this approach, we were able to characterise DNA fragments corresponding to putative escJ and escN genes in all DAEC isolates. Furthermore, DNA fragments homologous to the escC and escV genes were also amplified from all isolates. Besides the similarity found among the DAEC esc homologues with EPEC and EHEC esc genes, the nucleotide sequence analysis of the flanking regions of the amplified DNA fragments suggests that the putative DAEC esc genes are organised in the same manner as observed in EPEC and in EHEC strains. The results described here provide strong evidence for the presence of a TTSS in the DAEC strains analysed, implicating a pathogenic nature of these isolates. 相似文献
24.
25.
M. A. García-Pérez C. Climent P. Briones M. A. Vilaseca M. Rodés V. Rubio 《Journal of inherited metabolic disease》1997,20(6):769-777
Mutations P225L and P225R were identified in codon 225 of the gene for ornithine transcarbamylase (OTC) in two patients with the neonatal form of OTC deficiency. The mutations occur at a CpG dinucleotide and eliminate a unique MspI restriction site in exon 7 of the OTC gene. They do not alter existing splice sites or create new sites, as judged from the nucleotide sequence. Both mutations are associated with undetectable levels of OTC antigen in liver homogenates, and with either complete lack of OTC activity (P225R mutation) or very small residual activity (0.15% of normal in the P225L mutation). The residual activity observed with P225L exhibits normal pH dependence, little or no increases in the Km values for ornithine and carbamoyl phosphate and normal stability at either 37°C or, in the presence of 0.66 mol/L urea, at 0°C. The latter conditions were used to examine whether the P225L mutation favours dissociation of the active OTC trimer. Given the normal stability and lack of tendency to dissociation of the mutant enzyme, it appears likely that the dramatic reduction in the level of OTC protein is due to inefficient conversion of the mutant OTC precursor polypeptide (pOTC) into the correctly localized, appropriately folded, mature enzyme trimer, suggesting degradation of pOTC in transit to the mitochondria. 相似文献
26.
Effects of the MAO-A-inhibitor brofaromine (BRO), 10 mg/kg po after repeated (twice daily for 14 days) administration on the spontaneous behavior (exploratory and basal locomotor activities) and the exploratory activity modified by methoxamine, clonidine and d-amphetamine in male Wistar rats were studied in both light and dark phases of a diurnal cycle (L: 0700-1900 h). After single administration BRO in the light phase had no effects. In the dark phase BRO decreased the exploration (62% of control, p less than 0.01), increased the clonidine-evoked hypoactivity and amphetamine-evoked hyperactivity. The L-D differences occurred also after repeated administration. BRO in the light phase did not influence the exploration, decreased basal locomotor activity, did not change methoxamine and clonidine action and potentiated the action of amphetamine. In the dark phase, however, it did not influence the exploration and basal locomotor activity, intensified the methoxamine effect, and did not change the clonidine and amphetamine actions. The results demonstrate that the effects of BRO on behavior in rats: 1) differ from the effects caused by other antidepressants which are not MAO inhibitors; 2) are phase-dependent after both single and repeated administration. 相似文献
27.
The present authors investigated the excretion, distribution and pharmacokinetics of the novel potential antirheumatic agent flobufen and its active metabolite after p.o. and i.v. doses of 2, 10 and 50 mg/kg administered to rats. The drug is resorbed well from the digestive tract and mostly it is metabolized to the principal metabolite M, which is only slowly excreted from the organism mainly by renal clearance. Within the whole dose range the kinetics of the drug is linear. Binding of flobufen and M to proteins is high (95-99%). The highest concentrations of radioactive metabolites (mostly M) were found in the plasma, liver, lungs, kidneys, connective tissue and inflammatory foci. The penetration of metabolites through the placenta and excretion in human milk are relatively important. 相似文献
28.
29.
30.