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161.
Surface properties and cell response of low metal ion release Ti-6Al-7Nb alloy after multi-step chemical and thermal treatments 总被引:5,自引:0,他引:5
Ti-6Al-7Nb samples treated by innovative multi-step chemical and thermal processes were characterized in order to evaluate their surface properties and cell interaction. The main object was to asses if the treatments were effective in order to obtain a surface presenting at the same time bone-like apatite induction ability, low metal ion release, good cell response and high protein binding. The morphology, crystallographic structure, porosity and wettability of the treated materials were investigated, as well as their interaction with simulated body fluid during soaking for different times. Cytotoxicity, protein adsorption tests and in vitro fibroblast and osteoblast-like cell cultures were also performed. 相似文献
162.
Juan Bartulín Bernab Luis Rivas Mario Rodríguez-Baeza Ursula Angne 《Macromolecular chemistry and physics.》1982,183(12):2935-2940
Linear poly(iminoethylene) was synthetised by cationic polymerization of 2-methyl-2-oxazoline using BF3? O(C2H5)2, SnCl4, and CH3COBF4 as initiators and in the presence or absence of CH3CN. The resulting product, poly(N-acetyliminoethylene), was then hydrolysed in basic medium. The resulting poly(iminoethylene) was identified and characterized by 1H NMR, 13C NMR, X-ray diffraction, and differential scanning calorimetry (DSC). The synthetised polymer will be used as a polymeric support in ionic-exchange resins as well as in macromolecular pesticides. 相似文献
163.
Enrico M Trecarichi Mario Tumbarello de Gaetano Katleen Donati Enrica Tamburrini Roberto Cauda Christina Brahe Francesco D Tiziano 《AIDS research and therapy》2006,3(1):22-4
Despite multiple sexual exposure to HIV-1 virus, some individuals remain HIV-1 seronegative (exposed seronegative, ESN). The
mechanisms underlying this resistance remain still unclear, although a multifactorial pathogenesis can be hypothesised. Although
several genetic factors have been related to HIV-1 resistance, the homozigosity for a mutation in CCR5 gene (the 32 bp deletion, i.e. CCR5-Delta32 allele) is presently considered the most relevant one. In the present study we
analysed the genotype at CCR5 locus of 30 Italian ESN individuals (case group) who referred multiple unprotected heterosexual intercourse with HIV-1 seropositive
partner(s), for at least two years. One hundred and twenty HIV-1 infected patients and 120 individuals representative of the
general population were included as control groups. Twenty percent of ESN individuals had heterozygous CCR5-Delta 32 genotype,
compared to 7.5% of HIV-1 seropositive and 10% of individuals from the general population, respectively. None of the analysed
individuals had CCR5-Delta 32 homozygous genotype. Sequence analysis of the entire open reading frame of CCR5 was performed in all ESN subjects and no polymorphisms or mutations were identified. Moreover, we determined the distribution
of C77G variant in CD45 gene, which has been previously related to HIV-1 infection susceptibility. The frequency of the C77G
variant showed no significant difference between ESN subjects and the two control groups. 相似文献
164.
Comporti M Arezzini B Signorini C Sgherri C Monaco B Gardi C 《Laboratory investigation; a journal of technical methods and pathology》2005,85(11):1381-1391
Carbon tetrachloride (CCl4)-induced hepatic fibrosis has been considered to be linked to oxidative stress and mediated by aldehydic lipid peroxidation products. In the present study, we investigated whether collagen synthesis is induced by F2-isoprostanes, the most proximal products of lipid peroxidation and known mediators of important biological effects. By contrast with aldehydes, F2-isoprostanes act through receptors able to elicit definite signal transduction pathways. In a rat model of CCl4-induced hepatic fibrosis, plasma F2-isoprostanes were markedly elevated for the entire experimental period; hepatic collagen content also increased. When hepatic stellate cells (HSCs) from normal liver were cultured with F2-isoprostanes in the concentration range found in the in vivo studies (10(-9)-10(-8) M), a striking increase in DNA synthesis (reversed by the thromboxane A2 antagonist SQ 29 548), in cell proliferation and in collagen synthesis was observed. Total collagen content was similarly increased. Moreover, F2-isoprostanes markedly increased the production of transforming growth factor-beta1 by U937 cells, considered a model of liver macrophages. The data provide evidence for the possibility that F2-isoprostanes generated by lipid peroxidation in hepatocytes mediate HSC proliferation and collagen production seen in hepatic fibrosis. 相似文献
165.
Mario Cocco Cristiana Bellan Roxane Tussiwand Davide Corti Elisabetta Traggiai Stefano Lazzi Susanna Mannucci Lucio Bronz Nazzareno Palummo Chiara Ginanneschi Piero Tosi Antonio Lanzavecchia Markus G. Manz Lorenzo Leoncini 《The American journal of pathology》2008,173(5):1369-1378
Recent studies suggest that Epstein-Barr virus (EBV) can infect naïve B cells, driving them to differentiate into resting memory B cells via the germinal center reaction. This hypothesis has been inferred from parallels with the biology of normal B cells but has never been proven experimentally. Rag2−/− γc−/− mice that were transplanted with human CD34+ cord blood cells as newborns were recently shown to develop human B, T, and dendritic cells, constituting lymphoid organs in situ. Here we used this model to better define the strategy of EBV infection of human B cells in vivo and to compare this model system with different conditions of EBV infection in humans. Our results support the model of EBV persistence in vivo in cases that were characterized by follicular hyperplasia and a relatively normal CD4+ and CD8+ T-cell distribution. Intriguingly, in cases that were characterized by nodular and diffuse proliferation with a preponderance of CD8+ T cells, similar to infectious mononucleosis, EBV still infects naïve B cells but also induces clonal expansion and ongoing somatic mutations without germinal center reactions. Our results reveal different strategies of EBV infection in B cells that possibly result from variations in the host immune response. Future experiments might allow understanding of the mechanisms responsible for persistent EBV infection and provide targets for more highly tailored therapeutic interventions. 相似文献
166.
Jonathan T. Sims Venkatesh Krishnan Ching-Yun Chang Sarah M. Engle Giacomo Casalini George H. Rodgers Nicoletta Bivi Brian J. Nickoloff Robert J. Konrad Stephanie de Bono Richard E. Higgs Robert J. Benschop Silvia Ottaviani Anabela Cardoso Ajay Nirula Mario Corbellino Justin Stebbing 《The Journal of allergy and clinical immunology》2021,147(1):107-111
167.
168.
Paolo Colombo Pierino Radici Sergio Custro Mario Ermoni 《Macromolecular chemistry and physics.》1977,178(1):1-10
Attempts were made to elucidate experimentally the behaviour of poly(oxymethylene)-diol [α-hydro-ω-hydroxypoly(oxymethylene)] in the etherification reaction in order to explain the decrease of the polymer molecular weight compared with that of the starting product and in order to determine the type of terminal groups and how the thermal resistance and alkali stability can be affected. In fact one finds that, in addition to the wanted terminal ether groups, formyl and orthoformyl (dialkoxymethyl) groups are formed having a different chemical resistance in comparison to that of the pure ethers, particularly in basic media. 相似文献
169.
U Di Mario G Tamburrano M Iavicoli W J Irvine 《Clinical and experimental immunology》1977,29(3):523-524
The sera of ten patients with unexplained hypoglycaemia were examined for antibodies to pancreatic islets. Antibodies to pancreatic A, B and D cells (ICAb) were detected in one patient with an associated gastrointestinal tumour. 相似文献
170.
Paola Vigan Ruggero Pardi Barbara Magri Mauro Busacca Anna Maria Di Blasio Mario Vignali 《American journal of reproductive immunology (New York, N.Y. : 1989)》1994,32(3):139-145
PROBLEM: Recent evidence emphasizes the role of natural killer cells (NKs) as potential effectors of peritoneal immune surveillance directed against the outgrowth of endometrial cells, refluxed with menstrual debris, in ectopic sites. This NK-mediated cytotoxicity toward autologous endometrial antigens seems to be significantly decreased in endometriosis patients. METHOD: We set up experiments to clarify which molecules are involved in NK-endome-trial cell interaction. In particular, we evaluated the surface expression and functional activity of intercellular adhesion molecule-1 (ICAM-1), a cell surface glycoprotein that has been identified as one of the ligands for lymphocyte function-associated antigen-1 (LFA-1), present on almost all leucocyte cell types. Immunofluorescence flow cytometry was used to assess ICAM-1 expression on resting and IL 1β-activated endometrial stromal cells in culture. Dermal fibroblasts were used as control cells. Cytotoxicity and binding assays by 51Cr release in presence and absence of a specific monoclonal antibody (mAb) against ICAM-1 were then performed in order to determine the effect of this molecule on NK-mediated cytotoxic and binding activity toward endometrial stromal cells. RESULTS: The results of this study indicated that ICAM-1 expression on endometrial stromal cells seems to be constitutively higher than on dermal fibroblasts and can be up-regulated upon exposure to IL 1β. Furthermore, a mAb against ICAM-1 strongly inhibits the binding but not the cytotoxicity of NKs toward endometrial cells. No difference in the expression of this molecule was observed throughout the cycle. CONCLUSIONS: The presence of ICAM-1 on human endometrium might relate to the action of the immunocompetent cells in human specific reproductive events. 相似文献