Techniques: promiscuous Galpha proteins in basic research and drug discovery

Assay technologies that measure the activation of heterotrimeric (alphabetagamma) G proteins by G-protein-coupled receptors (GPCRs) are well established within the pharmaceutical industry, either for pharmacological characterization or for the identification of natural or surrogate receptor ligands. Despite recent evidence indicating that GPCR-linked signalling events might not be mediated exclusively by G proteins, G-protein activation remains a common benchmark for assessing GPCR family members. Thus, assay systems that translate ligand-mediated modulation of GPCRs into G-protein-dependent intracellular responses still represent key components of both basic research and the drug discovery process. In this article, the current knowledge and recent progress of integrating Galpha subunits into assay systems for GPCR drug discovery will be reviewed. Emphasis is given to novel promiscuous and chimeric Galpha proteins. Because of their ability to interact with a wide range of GPCRs, such novel G proteins are likely to be incorporated rapidly into drug discovery programmes.     

Oncological care according Alfred Schütz

The study was realized among oncological nurses in their daily work routine and aimed to understand these professionals' subjective action, starting from their relation with patients, adopting a phenomenological reference framework based on the ideas of Alfred Schütz. The question: what does working in oncological care mean to you? Please describe, was used to collect statements, which were analyzed and clarified the typical action of a nurse caregiver in this daily routine. The study revealed that oncological care implies dealing with humans in a fragile situation; requires a relationship of affectivity; is care delivery that entails the genesis of professional burnout. Care delivery in oncology is highly complex, requiring a professional competence that goes beyond the technical-scientific sphere. Nursing professionals need to seek strategies which enable them to face the fatigue they are submitted to in their work.     

Home Care--configurating the care model and joining different interests/needs in the care sector

This study aims to analyze a private Home Care service from the perspective of the formation of the Care Model. Focus groups and semistructured interviews were chosen as instruments for data collection. Interviews were held among middle-level nursing workers and service coordinators. Empiric data were organized by means of the Collective Subject Discourse methodology. It was disclosed that the recognition of the constituent elements of the care model among the social agents involved in the project is essential to negotiate, unite and instrumentalize the possibility to attend different interests. Another factor that stood out was the power of Home Care to be a tool for reviewing the health/illness/care process concept and, thus, for promoting the incorporation of values that lead to behavioral changes, moving towards a care model that is committed to the establishment of a welcoming relation, characterized by commitment and responsibility for the health of clients.     

Immune response and alteration of pulmonary function after primary human metapneumovirus (hMPV) infection of BALB/c mice

Human metapneumovirus (hMPV), a recently identified virus, causes upper and lower respiratory tract diseases. In this study, we show that BALB/c mice inoculated with hMPV exhibited significant morbidity on 1--2 days post-infection, when airway obstruction was found. Increased airway hyper-responsiveness to metacholine was found on day 4 concurrent with lung viral replication. Both IgG1 and IgG2a hMPV-specific antibodies were found in sera, while interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) were found in bronchoalveolar lavage. Lung histology showed parenchymal pneumonia and increased lymphocytic infiltration. We present here an animal model that may be helpful in studying hMPV pathogenesis and evaluating the effects of vaccines.     

Safety of mefloquine in infants with acute falciparum malaria

We evaluated retrospectively the safety of mefloquine (25 mg/kg) for the curative treatment of uncomplicated Plasmodium falciparum malaria in 26 infants weighing <15 kg and managed during a 5-year period. Side effects were recorded in 30.8% of patients and consisted chiefly of mild and transient gastrointestinal symptoms. Mefloquine was safe in infants weighing <15 kg in our experience.     

Power spectral analysis of heart rate in relation to sleep position

We used spectral analysis of heart rate variability, as a measure of autonomic tone, to determine spectral power differences in infants sleeping supine and prone. We studied 29 infants with a birth weight of 1,915 +/- 939 g, at the postconceptional age of 36 +/- 2 weeks. We then calculated total power (TP), low-frequency power (LF, 0.03-0.15 Hz), and high-frequency power (HF, 0.5-1.0 Hz). TP corresponds to overall heart rate variability, LF to both sympathetic and parasympathetic activity, and HF to parasympathetic activity only. Median (25th, 75th percentile) TP (beats/min2) in the supine position was 32.60 (23.12, 59.90), which was significantly higher than the prone position of 25.87 (14.94, 35.57). Similarly, LF (beats/min2) in the supine position of 13.82 (8.63, 23.31) was significantly higher than the prone position of 9.79 (5.46, 14.33). No significant difference was seen in the HF. We conclude that the prone position is associated with decreased heart rate variability and probably decreased sympathetic tone, which imply decreased autonomic stability in this position.     

Refinement and validation of a French in-patient experience questionnaire

The objective of this study was to check psychometric properties of a French-language in-patient experience questionnaire in a test sample different from the development sample. The questionnaire was sent out to 5,736 in-patients, within two to four weeks of discharge from a teaching hospital of 2,200 beds. Overall 4,095 questionnaires (71.4 per cent) were returned. Of these, 3,879 questionnaires were analyzed. In principal component analysis, seven principal components accounted for 62.4 per cent of the total variance. Cronbach's alpha coefficient ranged from 0.62 to 0.90, with the exception of the seventh scale (convenience scale, two items, Cronbach = 0.39). The overall patient experience score increased with increasing patient age (except for patients older than 65), male sex, low education level, use of a single room, and prior stay in the department. It also differed with respect to patients' behavioral intentions, answers to an overall satisfaction item, and open-ended comments.