股浅静脉戴戒术治疗原发性下肢深静脉瓣膜功能不全

目的 用股浅静脉戴戒术治疗原发性下肢深静脉瓣膜功能不全。方法 本文应用股浅静脉戴戒术治疗原发性下肢深静脉瓣膜功能不全34例,并应用无创性光电容积描记（PPG）结合临床症状改善状况对手术疗效进行评估。结果 手术前后静脉返流时间（VRT）有显著差异（P＜0．01）,临床症状改善。结论 股浅静脉戴戒术是治疗下肢深静静脉瓣膜功能轻度不全的有效方法,PPG检查结合临床改善程度是判断手术疗效更有效的方法。     

微悬浮聚合法合成聚苯乙烯磁性微球

采用微悬浮聚合制备了聚苯乙烯磁性微球（简称磁球）。在苯乙烯磁流体中加入引发剂和交联剂,然后将此磁流体分散在水中,经过高速剪切乳化,形成较稳定的微悬浮液,然后进行聚合,可合成0.4μm-6μm,主要分布在0.7μm-2.0μm的磁性微球。利用激光粒度分布仪、透射电镜（TEM）以及热重仪（TG）研究了引发剂用量、初始单体用量、聚合温度、磁粉用量、二乙烯苯（DVB）用量等因素对磁球的粒径大小及其分布、凝结量以及磁含量的影响。     

外源性磷酸肌酸对开心手术病人的心肌保护作用

20例需行瓣膜置换手术的风湿性心脏瓣膜病患者 ,随机等分为 2组。实验组 (CP组 )于术前 2d静脉滴注护心通 ,总量为 4g ;对照组 (C组 )用 5 %G S滴注。结果示实验组的心脏自动复跳率明显高于对照组 ,而对正性肌力药物的需求、缺血再灌后的MDA活性增高及心肌超微结构的改变则较对照组明显减轻 ,两组间的心肌酶活性和心肌ATP含量差异无显著性。提示护心通是一有效的心肌保护药物     

毒毛旋花子苷K间歇疗法治疗冠心病心力衰竭的临床研究

目的 :评价毒毛旋花子K间歇疗法 (简称ISKT)治疗保持窦性心律的冠心病心力衰竭的疗效及可行性。方法 :2 0 0例窦性心律的冠心病心力衰竭患者分为两组 :A组 98例采用维持量地高辛治疗 ;B组 10 2例采用ISKT。结果 :心率 (HR)、左室射血分数 (LVET)及血压 (BP)治疗前与治疗后 3个月比较 ,在A组分别为 :88± 12及 6 8± 12 (次 /min ,P <0 .0 1) ;0 32± 12及 0 40± 12 (P <0 .0 1) ;12 6± 2 1/ 90± 6及 12 8± 2 1/ 80± 15 ,(mmHg,P >0 .0 5 ) ;在B组分别为 :90± 10及 70± 11(次 /min ,P <0 .0 1) ;0 .32± 0 .10及 0 48± 0 10 (P <0 .0 1) ;12 8± 2 0 / 91± 7及 110± 10 / 76± 10(mmHg ,P <0 .0 1)。治疗 3个月后两组间比较 :HR(P >0 .0 5 ) ;LVEF(P <0 .0 5 ) ,Bp(P <0 .0 0 1)。B组的心功能有较好的改善 ,因心绞痛而服用的硝酸甘油量较少 ,两组洋地黄中毒或过量发生率的差别无显著性的意义。结论 :对窦性心律的冠心病心力衰竭 ,ISKT是安全、有效、可行的一种治疗方法。     

Role of shear wave elastography measured in the flaccid state in predicting arteriogenic erectile dysfunction

The gold-standard method for diagnosing arteriogenic erectile dysfunction (AED) is the penile Doppler ultrasonography. We proposed a novel method for predicting AED using ultrasonic shear wave elastography (SWE) considering that the former was invasive and variable. A total of 98 male patients were enrolled in our study, referred for ED between December 2018 and October 2020. For comparison, we also included 42 volunteers from the Healthy Physical Examination Center of our hospital. The Penile Doppler Ultrasonography (PDU) and SWE were performed for all patients with the intracavernosal injection (ICI). We named three groups as AED group, nonvascular ED group and healthy controls group. No statistically significant differences were found among the three groups in terms of demographic and clinical characteristics. There were no significant differences in IIEF-5 between AED and nonvascular ED. A significant (r = 0.642, p < 0.0001) positive correlation between flaccid and erectile SWE was observed. With a cut-off value of 13.45 KPa, the area under curve, specificity, and sensitivity of the SWE values under the flaccid state in distinguishing AED from healthy subjects were 0.867, 0.786 and 0.896 respectively. The SWE value in the flaccid state can distinguish the AED from healthy subjects.     

Culture bovine prospermatogonia with 2i medium

Germplasm cryopreservation and expansion of gonocytes/prospermatogonia or spermatogonial stem cells (SSCs) are important; however, it's difficult in cattle. Since inhibitors of Mek1/2 and Gsk3β (2i) can enhance pluripotency maintenance, effects of 2i-based medium on the cultivation of bovine prospermatogonia from the cryopreserved tissues were examined. The testicular tissues of newborn bulls were well cryopreserved. High mRNA levels of prospermatogonium/SSC markers (PLZF, GFRα-1) and pluripotency markers (Oct4/Pouf5, Sox2, Nanog) were detected and the PLZF⁺/GFRα-1⁺ prospermatogonia were consistently identified immunohistochemically in the seminiferous cords. Using differential plating and Percoll-based centrifugation, 41.59% prospermatogonia were enriched and they proliferated robustly in 2i medium. The 2i medium boosted mRNA abundances of Pouf5, Sox2, Nanog, GFRα-1, PLZF, anti-apoptosis gene Bcl2, LIF receptor gene LIFR and enhanced PLZF protein expression, but suppressed mRNA expressions of spermatogonial differentiation marker c-kit and pro-apoptotic gene Bax, in the cultured prospermatogonia. It also alleviated H₂O₂-induced apoptosis of the enriched cells and decreased histone H3 lysine (K9) trimethylation (H3K9me3) and its methylase Suv39h1/2 mRNA level in the cultured seminiferous cords. Overall, 2i medium improves the cultivation of bovine prospermatogonia isolated from the cryopreserved testes, by inhibiting Suv39h1/2-mediated H3K9me3 through Mek1/2 and Gsk3β signalling, evidencing successful cryopreservation and expansion of bovine germplasm.     

METTL3-Mediated m6A mRNA Methylation Modulates Tooth Root Formation by Affecting NFIC Translation

N6-methyladenosine (m⁶A), as a eukaryotic mRNA modification catalyzed by methyltransferase METTL3, is involved in various processes of development or diseases via regulating RNA metabolism. However, the effect of METTL3-mediated m⁶A modification in tooth development has remained elusive. Here we show that METTL3 is prevalently expressed in odontoblasts, dental pulp cells, dental follicle cells, and epithelial cells in Hertwig's epithelial root sheath during tooth root formation. Depletion of METTL3 in human dental pulp cells (hDPCs) impairs proliferation, migration, and odontogenic differentiation. Furthermore, conditional knockout of Mettl3 in Osterix-expressing cells leads to short molar roots and thinner root dentin featured by decreased secretion of pre-dentin matrix and formation of the odontoblast process. Mechanistically, loss of METTL3 cripples the translational efficiency of the key root-forming regulator nuclear factor I-C (NFIC). The odontogenic capacity of METTL3-silenced hDPCs is partially rescued via overexpressing NFIC. Our findings suggest that m⁶A methyltransferase METTL3 is crucial for tooth root development, uncovering a novel epigenetic mechanism in tooth root formation. © 2020 American Society for Bone and Mineral Research (ASBMR).     

Pyriform sinus fistula in children: A comparison of endoscopic-assisted surgery and endoscopic radiofrequency ablation

BackgroundPyriform sinus fistula (PSF) is a rare congenital anomaly, and the preferred definitive treatment is yet to be verified. In this study, we investigated the treatment outcomes of PSF specifically comparing endoscopic-assisted surgery and endoscopic radiofrequency ablation (RA).MethodsThe medical records of patients treated for PSF at the Shanghai Children's Hospital between October 2016 and September 2019 were retrospectively evaluated.ResultsThere were 93 girls and 98 boys. The median age at onset and operation was 3 years and 5 years, respectively. Endoscopic-assisted surgery was performed in 143 patients. During the same period, RA was performed in 48 patients, and 10 of them concurrently underwent incision and drainage of neck abscesses. Longer hospital stay was found in the endoscopic-assisted surgery group than in the RA group (10.50 ± 3.93 vs. 5.02 ± 3.30 days, P < 0.001). Postoperative complications were not significantly different between the two groups, except for neck infection (0 vs. 8.3%, P = 0.004). After a median follow-up period of 21 months, no significant difference was found between the two groups in terms of recurrence (1.4% vs. 0, P = 0.560).ConclusionPatients treated with RA had a significantly shorter hospital stay than those treated with endoscopic-assisted surgery. Outcomes of endoscopic-assisted surgery and RA were not significantly different for the management of PSF and treatment method should be tailored to the patient.Level of evidenceIV.     

通脉解痉汤联合康复训练治疗脑卒中后下肢痉挛临床研究

目的：观察通脉解痉汤联合康复训练治疗脑卒中后下肢痉挛的临床疗效及对痉挛指数、运动功能的影响。方法：采用随机数字表法将76例脑卒中后下肢痉挛患者随机分为对照组与观察组各38例。对照组行常规脑卒中对症治疗和康复训练治疗,观察组在对照组基础上加用通脉解痉汤治疗。比较2组临床疗效和治疗前后中医证候积分、痉挛指数、运动功能。结果：治疗前,2组中医证候积分比较,差异无统计学意义（P>0.05）;治疗后,2组半身不遂、手足挛急、偏身麻木、舌强言謇、面色晦暗、手足肿胀等中医证候积分均降低（P<0.05）,且观察组低于对照组（P<0.05）。观察组治疗总有效率92.11%,高于对照组78.95%(P<0.05)。治疗前,2组肌张力、腱反射、阵挛等痉挛指数量表（CSI）评分比较,差异无统计学意义（P>0.05）;治疗后,2组肌张力、腱反射、阵挛CSI评分均降低（P<0.05）,且观察组低于对照组（P<0.05）。治疗前,2组Fugl-Meyer运动功能量表（FMA）评分比较,差异无统计学意义（P>0.05）;治疗后,2组FMA评分均升高（P<0.05）...