卵巢巧克力囊肿破裂致急腹症37例分析

对３７例经开腹手术和病理结果证实为卵巢巧克力囊肿破裂的病例进行分析。本病发病呈上升趋势,误诊率高,本文结果显示术前确诊１６例,确诊率为４３．２％,本病特点为月经期或月经周期后半期发生突发性下腹剧痛伴明显腹膜刺激症,后穹隆穿刺抽出咖啡色液体,且无闭经,阴道不规则出血及休克体征。２１例分别误诊为卵巢囊肿蒂扭转,黄体破裂,异位妊娠和急性阑尾炎,其中阑尾炎占８例,提示本症需与上述妇科急腹症和急性阑尾炎鉴别     

Cyanidin-3-O-Glucoside Ameliorates Palmitic-Acid-Induced Pancreatic Beta Cell Dysfunction by Modulating CHOP-Mediated Endoplasmic Reticulum Stress Pathways

Cyanidin-3-O-glucoside (C3G) is a natural colorant with anti-diabetic properties, while its underlying mechanisms remain far from clear. Here, we investigated the protective role of C3G on palmitic acid (PA)-induced pancreatic beta cell dysfunction and further decipher its possible molecular mechanisms. Both primary isolated mouse islets and the INS-1E cell were used, and treated with a mixture of PA (0.5 mM) and C3G (12.5 µM, 25 µM, 50 µM) for different durations (12, 24, 48 h). We found that C3G could dose-dependently ameliorate beta cell secretory function and further alleviate cell apoptosis. Mechanistically, the primary role of the PKR-like ER kinase (PERK) endoplasmic reticulum (ER) stress pathway was detected by RNA sequencing, and the PERK-pathway-related protein expression, especially the pro-apoptotic marker C/EBP homologous protein (CHOP) expression, was significantly downregulated by C3G treatment. The critical role of CHOP in mediating the protective effect of C3G was further validated by small interfering RNA. Conclusively, C3G could ameliorate PA-induced pancreatic beta cell dysfunction targeting the CHOP-related ER stress pathway, which might be used as a nutritional intervention for the preservation of beta cell dysfunction in type 2 diabetes mellitus.     

综合医院护士锐器伤影响因素调查分析

目的了解综合医院护士发生锐器伤的现状及影响因素，为护理管理提供理论依据。方法采用自行设计的护士职业锐器伤调查表，对5所综合医院977名在岗护理人员发生锐器伤情况进行问卷调查。结果97．5％护士曾发生锐器伤；人均年锐器伤的发生次数因科室、工作环境、职称的差别而存在差异（P〈0．05，P〈0．01）；多因素分析示工作环境、学历是影响护士锐器伤的主要因素。结论需注重护士职业安全管理，提高护士防护意识、改变不良个人行为和操作习惯，增加护理人力资源配置，以有效减少锐器伤的发生。     

风湿性心脏病心房颤动患者心房纤维化程度与心房颤动复发的相关性

目的探讨风湿性心脏病心房颤动患者心房纤维化程度与心房颤动复发的相关性。方法68例风湿性心脏病二尖瓣病变患者(心房颤动50例,窦性心律18例)行心脏外科手术时取右心耳组织。应用V-G染色法和VIDAS-21图像分析系统测量心房组织胶原容积分数(CVF)。结果心房颤动患者的心房组织CVF(15.89%±1.29%)明显高于窦性心律患者(8.46%±3.45%,P<0.01)。术后50例心房颤动患者46例转复为窦性心律,4例转复未成功;随访12月后,维持窦性心律者35例(76.07%),心房颤动复发11例(23.97%);心房颤动复发患者心房组织CVF、心房颤动持续时间和左心房内径明显大于维持窦性心律组(P<0.05,P<0.01)。结论心房纤维化程度是引起风湿性心脏病心房颤动患者复律后心房颤动复发的因素之一。     

Coexistence of primary central nervous system lymphoma and primary breast lymphoma: Clinical presentation,imaging features,and treatment management

The presence of synchronous dual hematological diseases is an uncommon finding. We report an unusual case of coexistence of primary central nervous system lymphoma and primary breast lymphoma without systemic involvement in an immunocompetent patient. To our knowledge a similar case has not yet been reported in the literature. We especially focus on presenting the imaging features, the associated clinical findings and treatment management of each entity, with the aim of raising awareness on these two rare types of lymphomas and the possibility of their coexistence.     

国产MALDI-TOF MS系统microTyper MS对革兰阴性菌的鉴定性能评估

目的 评估国产基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)系统microTyper MS鉴定革兰阴性菌的性能.方法 使用国产MALDI-TOF MS系统microTyper MS与进口质谱系统VITEK MS系统同步鉴定该实验室储存的标准菌株及2018年6月至2019年6月临床分离的革兰阴性菌株.鉴定结...