抑郁症患者述情障碍及其特征

目的 了解抑郁症患者的述情障碍及其特征,探讨述情障碍与抑郁症不同症状间的关系.方法 选用多伦多述情障碍量表20项 (TAS-20)中文版和汉密尔顿抑郁量表17项版本(HAMD-17),对82例抑郁症患者和80名健康志愿者进行了评定.结果 (1)抑郁组TAS-20总分和因子分均显著高于对照组(P＜0.001);(2)抑郁组TAS-20总分、情感辨别不能因子分和外向性思维因子分随受教育水平的增高逐渐降低,差异有显著性(P＜0.01,P＜0.05),而对照组不同教育水平者在情感辨别不能因子和情感描述不能因子上差异有显著性(P＜0.05);(3)重度组TAS-20总分和因子分均高于轻-中度组,两组差异有显著性(P＜0.05,P＜0.01);(4)TAS-20总分、情感描述不能因子和外向性思维因子与HAMD-17总分、焦虑/躯体化因子、睡眠障碍因子、阻滞因子和体重因子呈显著正相关关系(r=0.231～0.343,P＜0.05,P＜0.01).HAMD-17认知障碍因子与TAS-20总分和因子分相关关系均不显著(P＞0.05).结论 抑郁症患者存在述情障碍,可能为抑郁症的一种状态反应,其特征与抑郁症功能性躯体不适症状密切相关.     

儿童反复呼吸道感染中医药研究近况

儿童反复呼吸道感染（recurrent respiratory tract infections,RRTIs）是儿童时期的常见病、多发病,严重影响小儿的生长发育和身心健康,其发病机理极其复杂,临床的治疗方法也很多,现就近十年相关发病的病因病机、内治法、外治法、实验研究进行系统回顾。中医药治疗疾病注重整体观念和辨证论治,其防治该疾病有其独特的优势,但每位研究者都是用自己的疗效指标来评价自己的试验结果,制定一个公认的疗效标准是今后研究的重点。     

复方银耳糖浆对小鼠免疫功能的影响

中药复方银耳糖浆灌胃对小鼠吞噬细胞(Mφ)吞噬指数和吞噬率以及对 B 细胞溶血空斑数量,与对照组相比,有增强作用。在药物所致免疫低下小鼠中这种增强效应更加明显。     

老年人的颅顶慢电位阈值

应用测定颅顶慢电位(SVP)阈值的方法,研究了23～80岁健康人总计39人70耳的客观听力。结果表明听觉系统的老化在40岁即已开始,50岁后比较明显,并随年龄的增长而愈加明显。     

全基因组测序技术在结核病流行病学调查中的应用

目的 评估全基因组测序技术在结核病分子流行病学调查中的应用。方法 对2008-2012年在上海市两家结核病定点医院发现9名耐多药患者中分离的结核分枝杆菌具有相同的可变数目串联重复序列,本研究对此进行流行学调查,并对9株结核分枝杆菌进行全基因组测序,分析其传播关系。结果 全基因组序列分析将9株结核分枝杆菌分为两个有传播关系的网络,一个为包括7株结核分枝杆菌（5例和2例患者分别来自不同的医院）的大簇,一个为只有2株结核分枝杆菌的小簇。两个簇之间相差15个单核苷酸多态性（SNP）位点,提示两个簇的遗传距离相对较远,基于菌株SNP差异构建的传播链显示了每个簇内菌株的传播方向和耐药突变积累的过程。结论 基于全基因组测序数据研究耐药结核病的传播网络,能准确判断传播路径和方向,识别传染源和传播缺失环节。     

乳腺癌超声弹性成像与TLR9、Her-2和ER的相关性

目的 探讨乳腺癌的常规超声结合超声弹性成像的特征与Toll样受体9(Toll-Like Receptor 9,TLR9)、人表皮生长因子受体-2(Human epidermal growth factor receptor-2,Her-2)和雌激素受体(Estrogen Receptor,ER)的相关性.方法 收集牡丹...     

Highly covalent molecular cage based porous organic polymer: pore size control and pore property enhancement

It remains a great challenge to effectively control the pore size in porous organic polymers (POPs) because of the disordered linking modes. Herein, we used organic molecular cages (OMCs), possessing the properties of fixed intrinsic cavities, high numbers of reactive sites and dissolvable processability, as building blocks to construct a molecular cage-based POP (TPP-pOMC) with high valency through covalent cross coupling reaction. In the formed TPP-pOMC, the originating blocking pore channels of TPP-OMC were “turned on” and formed fixed pore channels (5.3 Å) corresponding to the connective intrinsic cavities of cages, and intermolecular pore channels (1.34 and 2.72 nm) between cages. Therefore, TPP-pOMC showed significant enhancement in Brunauer–Emmett–Teller (BET) surface area and CO₂ adsorption capacity.

By utilizing the cage to framework strategy, the blocking pores of the cage itself were “turned on” to construct a highly covalent molecular cage based porous organic polymer.     

SLC2A3 variants in familial and sporadic congenital heart diseases in a Chinese Yunnan population

BackgroundSolute carrier family 2 member 3 (SLC2A3), is a member of a superfamily of transport protein genes. SLC2A3 played an important role in embryonic development. Previous research reported SLC2A3 duplication was reportedly associated with congenital syndromic heart defects. However, it is not clear whether the gene is associated with non‐syndromic congenital heart disease. Our study aimed to elucidate the relationship between its variation and congenital heart disease.MethodsGenomic DNA extracted from the peripheral blood leukocytes of two families with CHD were sequenced with whole‐exome sequencing to identify variations, used Sanger sequencing to investigate SLC2A3 variants in 494 Chinese patients with CHD and 576 healthy unrelated individuals.ResultsIn members from the two families, three with CHD had the SLC2A3 (rs3931701) C > T variant. Of the 494 patients with CHD, 394 had gene variants (86 had the TT type and 308 had the CT type). Of the 576 healthy controls, 272 participants had gene variants (36 had the TT type and 236 had the CT type). The TT type [p < 0.0001, odds ratio (OR) =7.262, 95% confidence interval (CI) =4.631–11.388] and CT type (p < 0.0001, OR =3.967, 95% CI =2.991–5.263) of SLC2A3 (rs3931701) significantly increased the risk of sporadic ASD in a Chinese Yunnan population.ConclusionsSingle nucleotide variations of SLC2A3, particularly, the SLC2A3 (rs3931701) C > T variant increased the risk of CHD among the studied population.     

Simultaneous quantification of apolipoproteins A-I,E, and J in human plasma by LC-MS/MS for clinical application to diabetes mellitus complicated with cardiovascular disease

Apolipoproteins (Apos) play an important role in regulating plasma lipid concentration. Complex disorders of Apos are highly related with diabetes mellitus, cardiovascular and other diseases. Direct measures of lipoprotein fractions for risk assessment suffer from inaccuracy in the dyslipidemia and pathological states. Therefore, a reliable precise assay will be of high clinical utility. LC-MS/MS methods with multiple reaction monitoring modes have proven suitable for multiplexed quantification. We aimed to develop a simple, cost-effective and amenable LC-MS/MS assay for quantification of ApoA-I, ApoE and ApoJ in human plasma. Standards were constructed from substitute matrix and proteotypic peptides for external calibration and corresponding stable isotope labeled peptides were added as internal standards to remove matrix effects. Analytical validation of the assay included the assessment of linearity, accuracy (RE: −3.02% to 5.32%), intra-assay precision (RSD: 2.50% to 6.56%), inter-assay precision (RSD: 0.78% to 6.68%), spiking recovery rate (accuracy: 87.17% to 112.71%), matrix effect (accuracy: 88.03% to 114.87%), and reproducibility and repeatability of sample preparation (RSD: 1.95% to 7.26%). The performance of proteotypic peptides ApoA-I, ApoE and ApoJ was sufficient for triplex quantitation within a linear range from 16.26 to 1626.41 pmol mL⁻¹, 1.03 to 103.35 pmol mL⁻¹ and 0.86 to 86.46 pmol mL⁻¹ respectively. For all quantified peptides, the determination coefficient (R²) was >0.997. Besides, the validated LC-MS/MS method has been successfully applied to the quantification of plasma samples in diabetes mellitus and cardiovascular diseases. We anticipate that this assay may provide an alternative method for future clinical applications.

Simultaneous quantification of apolipoproteins A-I, E, and J in human plasma by LC-MS/MS.     

"三资"企业卫生防护效果10年对比分析

目的 对企业作业场所劳动卫生状况进行对比分析，研究“三同时”卫生防护效果的持续性，探讨最佳防护效果的方法。方法 以1993年6月底竣工投产的42家新、改、扩建“三资”企业为研究对象，对比不同行业卫生防护效果，10年间技术改革及管理状况对卫生防护效果的影响。结果 (1)化工、玩具行业作业点毒物合格率、机械行业作业点粉尘合格率下降明显。(2)10年间无整改或技改且管理欠佳的11家企业10年后毒物和粉尘作业点合格率下降明显。(3)采用机械自动化、湿式作业、密闭通风防护措施的防护效果及持续性好于局部通风。结论 科学设计是提高和保持卫生防护效果的关键，管理维护工作是使卫生防护设施长期有效运转的保障。