Increased frequency of CD4+ CD25+ FOXP3+ cells correlates with the progression of 4-nitroquinoline1-oxide-induced rat tongue carcinogenesis

Objectives

CD4+ CD25+ FoxP3+ T cells (Tregs) play an essential role in sustaining self-tolerance by negatively regulating immune responses. Increased frequencies of Tregs have been reported in a variety of human cancers. The aim of this study was to evaluate the prevalence of Tregs infiltration in the peripheral blood and regional lymph nodes during rat tongue carcinogenesis induced by 4-nitroquinoline-1-oxide (4NQO).

Materials and methods

Forty-eight Sprague–Dawley rats were divided into the control (n?=?16) and experimental groups (n?=?32) to which 4NQO in drinking water was administered. Flow cytometry was used to analyze the prevalence of Tregs in lymphocytes of peripheral blood and regional lymph nodes during 4NQO-induced rat tongue carcinogenesis. CD4+ CD25+ FoxP3+ cells were expressed as a percentage of the total CD4+ cells.

Results

The frequency of Tregs in peripheral blood from squamous cell carcinoma rats was significantly higher than controls (3.82?±?0.62 versus 1.40?±?0.31 %, P?P?Conclusion and clinical relevance Tregs in peripheral blood and lymph nodes were associated with disease progression during 4NQO-induced rat tongue carcinogenesis. This study indicated that the upregulation of Tregs might play important role during oral mucosa malignant transformation.     

miRNA在食管癌中的研究进展

我国是食管癌高发国家,发病人数占全球一半以上,国内食管癌的死亡率为17.38/10万,居恶性肿瘤的第4位。miRNA是新发现的一种具有高度进化保守性的单链RNA,在转录后水平引起靶mRNA降解或翻译抑制。miRNA的表达水平与食管癌的类型、预后等密切相关。在外周血中,miRNA水平也表现出显著的肿瘤相关性、组织特异性和表达稳定性。随着食管癌发病率不断增加,人们更加关注食管癌的发病机制及早期分子生物学标志物的研究。     

Moderate-dose glucocorticoids as salvage therapy for severe pneumonia in renal transplant recipients: a single-center feasibility study

This study aimed to assess the effectiveness and safety of moderate-dose glucocorticoids (GCs) with mechanical ventilation as salvage therapy for renal transplant recipients with severe pneumonia, which was non-responsive to conventional treatment. A retrospective study was conducted involving renal transplant recipients diagnosed with severe pneumonia and did not respond to conventional treatment. All immunosuppressants were then completely withdrawn, and the patients were initially administered with methylprednisolone at doses of 2.0–2.5?mg/kg/day once every 12?h. This dosage was continued until oxygenation improved, and the treatment was gradually tapered (by 20?mg every 2–3 days) to the previous maintenance dosage. Ten patients were recruited from year 2008 to 2012. Two patients who underwent emergency endotracheal intubation were intubated on days 3 and 8, respectively, another one died from recurrent pneumothorax. The mean PaO₂/FiO₂ of the nine survivors was significantly increased by the increasing treatment duration; whereas the lung injury scores (LIS) and the sequential organ failure assessment (SOFA) score were both significantly decreased. The use of moderate-dose GCs may play a role as salvage therapy for renal transplant recipients with severe pneumonia. However, further study with larger trials to is needed.     

危重病患者血清瘦素表达及营养评价指标的作用

目的　探讨危重患者急性期禁食、全胃肠外营养 (TPN)治疗后血清瘦素的表达 ,以及营养评价指标对营养支持的作用。方法　用放射免疫法测定危重病患者血清瘦素 ,同时检测相关营养评价指标。结果　危重病患者急性期瘦素变化不明显 ,TPN治疗后迅速上升 ,第 3天达高峰 ,治疗后 5d仍高于正常。两组血浆纤维蛋白原、白蛋白、总胆固醇、甘油三脂、血淋巴细胞计数差异均无显著性意义 ,P >0 0 5。结论　危重病患者急性期禁食血清瘦素无显著性改变 ,但对TPN治疗反应迅速 ,血清瘦素有可能用来作为危重病患者急性期营养评价指标。血浆纤维蛋白原、白蛋白、总胆固醇、甘油三脂、淋巴细胞计数对危重病患者急性期营养状况无评价性作用。