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101.
我们制定了制备抗-SED血清的免疫方案,抗原用量6.9mg/兔,注射8针,免疫时间共14周。实验表明,抗-SED血清效价可达1∶128,所制备的抗血清与纯度高(98%)及纯度较低(50%~60%)的SED肠毒素都只产生1条沉淀线,并且融合,同SEA、SEB、SEC_I、SEC_Z均没有交叉反应。硫铵盐析沉淀法提取的抗-SED IgG,用于致敏醛化血球,该抗体诊断致敏血球检出SED灵敏度较高,约1ng/ml。同时用该抗体诊断血球对6种罐头食品人工污染SED肠毒素进行了检验,检出灵敏度为0.78~3.12ng/ml,略低于含纯SED标本,食物成分对反向间接血凝没影响,不出现假阳性和假阴性。效价高或效价低的抗-SED血清都可用于致敏醛化血球。 相似文献
102.
Wang Tao Liang Zhihou Sun Shenggang Cao Xuebing Peng Hai Cao Fei Liu Hongjin Tong E-tang 《华中科技大学学报(医学英德文版)》2003,23(2):145-147
Summary To investigate the distribution of possible novel mutations from parkin gene in variant subset of patients with Parkinson’s
disease (PD) in China and explore whether parkin gene plays an important role in the pathogenesis of PD, 70 patients were
divided into early-onset group and late-onset group; 70 healthy subjects were included as controls. Genomic DNA from 70 normal
controls and from those of PD patients were extracted from peripheral blood leukocytes by using standard procedures. Mutations
of parkin gene (exon 1–12) in all the subjects were screened by PCR-single strand conformation polymorphism (SSCP), and further
sequencing was performed in the samples with abnormal SSCP results, in order to confirm the mutation and its location. A new
missense mutation Gly284Arg in a patient and 3 abnormal bands in SSCP electrophoresis from samples of another 3 patients were
found. All the DNA variants were sourced from the samples of the patients with early-onset PD. It was concluded that Parkin
point mutation also partially contributes to the development of early-onset Parkinson’s disease in Chinese.
WANG Tao, male, born in 1961, Associate Professor
This work was supported by grants from the key program of the special scientific project of Scientific & Technologic Agency
of Hubei Province (Serial No. 2001AA308B01) and the Hygienic Research Project of Hygienic Agency of Hubei province (Serial
No. WJ 01529). 相似文献
103.
止血灵(暂定名)是由尖吻腹蛇(Deina Agkistrodon acutus)蛇毒分离提取得到的一种类凝血酶组分,具有明显的促凝(止血)作用,我科自1992年10月~1993年10月使用广州蛇毒研究所与广州医学院血液学研究 相似文献
104.
105.
登革病毒感染后小鼠细胞免疫功能的动态变化 总被引:2,自引:0,他引:2
从登革Ⅱ型病毒感染成年BALB/c小鼠为模型,观察感染后不同时间机体细胞免疫反应的变化。结果表明:感染后7-14d免疫功能处于激活状态,表现为腹腔巨噬细胞的吞噬功能和脾淋巴细胞对ConA的反应及IL-2产生水平都明显高于对照组,感染21d后免疫功能则转为抑制状态,上棕免疫反应明显低于对照组,脾中的L3T4^+细胞亚群的百分比逐渐下降;相反,LYT2^+细胞亚群的百分比逐渐升高,这种抑制现象可维持到 相似文献
106.
L H Daltroy C B Phillips H M Eaton M G Larson A J Partridge M Logigian M H Liang 《American journal of public health》1995,85(4):558-560
The Physical Capacity Evaluation, a performance measure of functional capabilities comprised of 13 tasks simulating those used in activities of daily living, was tested on 289 community-dwelling elderly people and compared against a widely used self-report measure of function, the Health Assessment Questionnaire. Factor analysis identified one dominant component in each instrument. Internal consistency reliability (Cronbach's alpha) was .90 for both instruments. Global disability (Health Assessment Questionnaire) and function (Physical Capacity Evaluation) scores were correlated -.74. One-week retest reliabilities on 58 subjects were .94 for the Physical Capacity Evaluation and .95 for the Health Assessment Questionnaire. The Physical Capacity Evaluation is a valid and reliable measure of physical performance for use with elderly people. 相似文献
107.
108.
目的:观察吸入一氧化氮(NO)对心瓣膜置换术后肺动脉高压患者血流动力学的影响。方法:选择9例心瓣膜置换术后伴肺动脉高压的病人,吸入NO0.003%,观察三个时象点:吸入NO前;开始吸入NO后15分钟;停止吸入NO后15分钟。结果:吸入NO能显著降低肺动脉压和肺循环阻力指数(P<0.01),停止吸入NO15分钟后,肺动脉压和肺循环阻力指数恢复到原有水平。在整个观察过程中,心率、平均动脉压、中心静脉压、肺动脉楔压、体循环阻力指数和心脏指数均无显著变化(P>0.05)。结论:吸入NO具有选择性肺血管扩张作用,是治疗心瓣膜置换术后肺动脉高压的较理想药物。 相似文献
109.
BACKGROUND: Chronic renal failure (CRF) is associated with an atherogenic
lipid profile and an increased risk of ischaemic cardiovascular disease.
The associated hyperlipidaemia is reportedly ameliorated by erythropoietin
(Epo) therapy. According to a recent report, rats studied 3 weeks after 5/6
nephrectomy and fed a high- protein diet exhibited increased activities of
hepatic HMG-CoA reductase (HMG-CoAR) and cholesterol 7 alpha-hydroxylase
(Ch-7 alpha- H), despite normal corresponding mRNA values. DESIGN AND
METHODS: This study was designed to examine the effects of naturally
progressing CRF of longer duration as well as those of Epo therapy on gene
expressions of the key factors involved in hepatic cholesterol metabolism,
i.e., LDL receptor (LDLR), HMG-CoAR, and Ch-7 alpha-H. Sprague-Dawley rats
were randomized to the CRF group (5/6 nephrectomy), Epo-treated CRF group
(given Epo 150 U/kg/twice weekly) and sham-operated, placebo- treated
normal controls. They were allowed free access to regular rat chow and
studied 6 weeks after surgery. Liver mRNAs and protein mass or activities
of the above factors were studied. RESULTS: Plasma cholesterol
concentration was significantly increased in the CRF group (P < 0.001)
and was modestly lowered (P < 0.05) by Epo therapy. However, microsomal
cholesterol concentration and LDLR, HMG-CoAR, and Ch-7 alpha-H mRNA as well
as HMG-CoAR activity, and Ch-7 alpha-H and LDLR protein mass measurements
were virtually identical in the three groups. Thus, hepatic LDLR, HMG-CoAR,
and Ch-7 alpha-H mRNA and protein measurements in rats with CRF were
similar to those of the normal control group representing an inappropriate
response to the associated hypercholesterolemia. Epo therapy led to partial
amelioration of CRF- associated hypercholesterolaemia with no discernible
effect on hepatic tissue expression of the above factors.
相似文献
110.
目的评价磁共振尿路成像(Magneticresonanceurography,MRU)对泌尿系疾病的诊断价值。方法对36例泌尿系疾病患者进行MRU检查,并结合临床手术、病理资料和其他影像学资料进行回顾性分析。结果36例患者中,均有MRU阳性结果,3例肾盂、肾盏癌,4例前列腺癌,8例前列腺增生症,2例输尿管癌定性、定位明确;9例肾盂、输尿管移行段梗阻定位明确,定性有帮助;10例输尿管下段梗阻中6例诊断明确,4例未能确诊。结论MRU在泌尿系疾病的诊断中,对肿瘤的定位、定性参考价值较高,对尿路梗阻定位诊断有价值,对定性诊断帮助有其局限性。 相似文献