培养环境的温度变化与人MⅡ期卵母细胞纺锤体形态学变化的关系

目的:观察环境温度的改变对MⅡ期人卵纺锤体结构的影响.方法:40例非男性因素的不孕妇女,将常规体外授精后未受精的MⅡ期卵子置于室温(23～25℃)直至纺锤体消失.于纺锤体消失后,将卵子分成3个实验组:1组纺锤体消失后立即复温(n=8);2组纺锤体消失后室温下静置5 min再复温(n=6);3组纺锤体消失后室温下静置10 min再复温(n=6).卵子复温后5 min,10 min,2 h分别观察纺锤体的复现情况.结果:20个未受精的MⅡ期卵子置于室温下5 min内纺锤体均消失.1组中的8个卵子,2组中的6个卵子复温后再次观测到了MⅡ期纺锤体.3组中的6个卵子只有3个卵子再次出现了MⅡ期纺锤体.对照组的18个卵子放置于37℃恒温板上观察30 min,纺锤体均未见消失.结论:MⅡ期卵子纺锤体对环境温度的改变非常敏感.温度降低将导致纺锤体消失,随着时间的延长,纺锤体复现几率明显减少.     

浅谈基因治疗的现状及其面临的困境

基因治疗将在未来的疾病治疗中扮演重要的角色，然而这一新颖的技术却面临着诸多问题，本文论述了基因治疗的发展现状及其所面临的技术难题。     

Distribution of rat facial motoneurons and its reorganization following nerve reinnervation]

OBJECTIVE: To examine the distribution of rat facial motoneurons contributing different branches under normal situation and when nerve reinnervation occurred following facial nerve axotomy. METHODS: The normal distribution of motoneurons innervated both buccal and marginal mandibular branches and its reorganization after facial nerve reinnervation was observed using retrograde labeling with fluorescein. RESULTS: Under normal situation, the motoneurons contributing buccall and marginal mandibular branches were primarily distributed in the intermedial and lateral subnucleus in facial nucleus and almost completely overlapped. The two types labeled neurons organized closely, but there were no double-labeled neurons. Although the motoneurons contributing buccall and marginal mandibular branches were primarily overlapped 4 month post-anastomosis, the number of the labeled neurons obviously decreased and the organization got more scattered. There were 10% of buccall branches, 5% of marginal mandibular motoneurons in the dorsal subnuleus, 1% of buccall and 4% of marginal mandibular in dorsal ventral and medial subnucleus. The distribution pattern of the motoneurons 6 month post-anastomosis was similar to that of 4 month post-anastomosis, but the number of the labeled neurons increased, and there were 1%-2% double-labeled neurons. CONCLUSION: The distribution pattern of motoneurons innervated both buccal and marginal mandibular branches indicates that it should exist wide-spread communicating branches, and its reorganization after facial nerve reinnervation suggests that misdirected regeneration occurs among motoneurons innervating different branches.     

辣椒中辣椒素含量的测定

本文作者改进了辣椒中辣椒素含量的测定法。即通过增设洋品空白管以消除色素的干扰;改变 Na_2CO_3溶液的浓度以缓和其操作条件及防止在低温时产生沉淀。本改良法具有简便、快速和重现性好之优点。     

全营养素混合液(TNA)在肠外营养的应用

脂肪乳剂、葡萄糖、氨基酸、电解质、维生素及微量元素按一定程序混合配成10种全营养素混合液。在4℃14天贮存期内,10种混合液的pH和渗透压变化不大,不同混合液在不同时期的脂粒平均直径变化为0.3135±0.1012μm～0.4813±0.1893μm,94.93％脂粒的直径<0.6μm,无直径>6μm的脂粒,细菌培养阴性。74例共输全营养素混合液1,796次,无不良临床反应和异常实验室检查结果;用1周以上者大都显示氮平衡改善,用2周以上者血清转铁蛋白和白蛋白浓度可有不同程度升高。