Expression of RRM1 and RRM2 as a novel prognostic marker in advanced non-small cell lung cancer receiving chemotherapy

The aim of this study was to examine the prognostic value of BRCA1, RRM1, and RRM2 in patients with non-small cell lung cancer (NSCLC) who received adjuvant chemotherapy. A total of 418 patients who underwent curative pulmonary resection were obtained between January 2007 and November 2009. The relative cDNA quantification for BRCA1, RRM1, and RRM2 was conducted using a fluorescence-based, real-time detection method, and β-actin was used as a reference gene. The low expression of RRM1 and RRM2 significantly increased the platinum-based chemotherapy response (For RRM1: odds ratio (OR)?=?2.09, 95 % confidence interval (CI)?=?1.38–3.18; For RRM2: OR?=?1.64, 95 % CI?=?1.09–2.48). The univariate analysis indicated that low expression of RRM1 attained a longer time to progression and overall survival time, with HR (95 % CI) of 0.50 (0.33–0.77) and 0.60 (0.39–0.92), respectively. Similarly, low expression of RRM2 had a longer time to progression and overall survival, with HR (95 % CI) of 0.57 (0.38–0.86) and 0.47 (0.31–0.71), respectively. In conclusion, low expression of RRM1 and RRM2 could be used to predict the treatment response to platinum-based chemotherapy and survival in NSCLC. The RRM1 and RRM2 could substantially contribute to the future design of individualized cancer treatment in NSCLC patients.     

医学伦理与老龄保健事业

本文简要介绍医学伦理与医疗卫生工作,特别是老龄保健事业之间的紧密联系,指出医学伦理要求医务人员在医疗实践中应遵循的三个基本原则。医务人员应将患者的利益放在首位,处理好医患关系。由于我国人口众多,加之年龄日益老化,进入新世纪后,繁重的老龄保健事业对医疗卫生工作提出更高要求,同时也使医学伦理面临新的挑战和机遇。     

Unusual hyperpigmented patches,an undeveloped breast and a cataract in a female with incontinentia pigmenti

A 19‐year‐old female and her mother visited our department with a history of unusual reticular brown‐black patches on their trunks and limbs since infancy. Besides the obviously fulsome hyperpigmentation, the daughter also showed other unusual clinical manifestations such as generalized hypohidrosis, especially on the hyperpigmented patches, cicatricial alopecia, a cataract on her right eye, abnormal teeth and a right dysplastic breast. The mother had a median diastema between her maxillary central incisors, hypoplasia of the enamel, hypohidrosis and hyperpigmented patches on her left thoracic region. Analysis of the NEMO (NF‐κB essential modulator) gene in the patient and her mother revealed a deletion of exons 4–10. Their EDA and EDAR genes were normal.     

Short-term environmental enrichment exposure induces proliferation and maturation of doublecortin-positive cells in the prefrontal cortex

Previous studies have demonstrated that doublecortin-positive immature neurons exist pre- dominantly in the superficial layer of the cerebral cortex of adult mammals such as guinea pigs, and these neurons exhibit very weak properties of self-proliferation during adulthood under physiological conditions. To verify whether environmental enrichment has an impact on the proliferation and maturation of these immature neurons in the prefrontal cortex of adult guinea pigs, healthy adult guinea pigs were subjected to short-term environmental enrichment. Animals were allowed to play with various cognitive and physical stimulating objects over a period of 2 weeks, twice per day, for 60 minutes each. Immunofluorescence staining results indicated that the number of doublecortin-positive cells in layer II of the prefrontal cortex was significantly increased after short-term environmental enrichment exposure. In addition, these doublecortin-positive cells co-expressed 5-bromo-2-deoxyuridine （a marker of cell prolifera- tion）, c-Fos （a marker of cell viability） and NeuN （a marker of mature neurons）. Experimental findings showed that short-term environmental enrichment can induce proliferation, activation and maturation of doublecortin-positive cells in layer II of the prefrontal cortex of adult guinea pigs.     

MiR-876-5p acts as an inhibitor in hepatocellular carcinoma progression by targeting DNMT3A

Hepatocellular carcinoma (HCC) is one of the biggest challenges that human beings faced with in 21st century. Previous researches have revealed that miRNAs can serve as regulators in various cancers. MiR-876-5p, a member of miRNA family, has been studied in lung cancer for its anti-oncogenic function. However, the exact function of it is not reported in HCC. Our study aims to find out the effects of miR-876-5p expression on HCC progression. Two HCC cells were chosen to do functional assays after miR-876-5p expression was detected in cell lines by qRT-PCR. HepG2 cell was transfected with miR-876-5p mimics, whereas LM3 cell was transfected with miR-876-5p inhibitors. Next, cell activities of these two indicated cells were analyzed by means of MTT assay, colony forming assay, transwell migration assay and western blot analysis. Consequently, we found that miR-876-5p could inhibit both cell proliferation and metastasis. Moreover, we found out a target gene (DNMT3A) of miR-876-5p by performing bioinformatics analysis, dual luciferase reporter assay and biotin-avidin pull-down assay. Finally, rescue assays were carried out in HepG2 cells. We found that DNMT3A could reverse miR-876-5p mimics-induced inhibition. Therefore, we concluded that miR-876-5p suppressed hepatocellular carcinoma progression by targeting DNMT3A.     

在医学院校开展性教育的探索与思考

总结了在医学院校开展性教育11年来的经验,探讨了大学阶段开展性教育的必要性,大学性教育的目标和内容选择以及大学性教育的适宜方式和途径等问题.     

红细胞压积与白蛋白差值在继发性腹腔感染患者病程中的变化

  目的   通过观察HCT和ALB差值在腹腔感染患者治疗过程中的变化，为腹腔感染患者的准确治疗提供一定的指导。   方法   选取2018年1月至2018年12月昆明医科大学第一附属医院收治的各类腹腔感染手术患者共计106名，按手术时间分为术前、术后12 h、术后1 d术后2 d、术后7 d、术后14 d和术后21 d 7组（n = 106），记录患者性别、年龄；收集患者上述各时间点患者的红细胞压积、血浆白蛋白，计算HCT-ALB差值。再根据临床结局分为术后好转组和死亡组。比较每组HCT、ALB和HCT-ALB差值。   结果   与手术前相比，术后12 h、术后1 d、术后2 d和术后7 d，患者HCT和ALB下降，HCT-ALB增大（P < 0.05）；与手术前相比，术后14 d，HCT和ALB下降，（P < 0.05），HCT-ALB差异无统计学意义（P > 0.05）；与好转组相比，死亡组患者年龄增加（P < 0.05），术前的HCT-ALB增大（P < 0.05）。   结论   腹腔感染患者在手术治疗后毛细血管渗漏加重，这一病理过程可持续7 d以上，死亡患者的毛细血管渗漏更严重。     

运动学对线与机械对线全膝关节置换早期临床结果比较

目的探讨并对比分析应用专用工具的运动学对线全膝关节置换和机械对线全膝关节置换的早期临床结果。 方法回顾性分析2019年3月至2021年3月间，在首都医科大学附属北京朝阳医院因膝骨关节炎行单侧全膝关节置换术，术后随访时间大于3个月且随访资料完整的患者，排除类风湿性关节炎等炎性关节病、髌股关节严重对位不良以及既往有膝关节侧副韧带或后交叉韧带损伤的患者；按对线方法分为专用工具运动学对线全膝关节置换(KA-TKA)组和机械对线全膝关节置换(MA-TKA)组，记录手术前后髋－膝－踝(HKA)角度，术前及术后3个月的膝关节临床评分(KS-C)和膝关节功能评分(KS-F)，术后3个月的遗忘关节评分(FJS)，采用独立样本t检验行统计学分析。 结果共有162例患者纳入研究，其中KA-TKA组82例，MA-TKA组80例。两组患者术后HKA差异无统计学意义(P>0.05)；术后3个月KA-TKA组患者的KS-C评分(t＝2.259)、KS-F评分(t＝1.994)和FJS评分(t＝2.945)均高于MA-TKA组，两组间差异有统计学意义(均为P<0.05)。 结论KA-TKA与MA-TKA相比，术后下肢力线相似，具有更好的临床评分表现和更高的膝关节功能表现，以及更高的FJS评分。     

锁定加压钢板治疗股骨转子间骨折21例临床分析

目的:观察锁定加压钢板治疗股骨转子间骨折的疗效.方法:应用锁定加压钢板治疗股骨转子间骨折21例.结果:21例患者3～ 6个月骨折均愈合,未出现断钉、内固定松动等并发症,无明显髋内翻和下肢短缩畸形;Harris评分为优11例,良8例,可2例.结论:锁定加压钢板治疗股骨转子间骨折具有容易安装、固定可靠、手术时间短、出血量少、髋关节功能恢复优良率高等特点,是一种有效的手术方法.