脐血抗—HPCA—2和Tük3标记的CD34阳性细胞的流式细胞仪比较分析

在标记脐血造血祖细胞表面抗原（ＨＰＣＡ），ＣＤ３４中，比较抗－ＨＰＣＡ－２－ＦＩＴＣ和Ｔｋ３（纯抗体）标记的ＣＤ（３４＋）细胞在流式细胞仪分析中的荧光特征及两种单抗标记的ＣＤ（３４＋）细胞与体外培养的粒单细胞集落形成单位（ＣＦＵ－ＧＭ），红系爆发形成单位（ＢＦＵ－Ｅ），和混合集落形成单位（ＣＦＵ－Ｍｉｘ）的相关性。结果发现脐血有核细胞中，抗ＨＰＣＡ－２阳性细胞占１．０５±０．７２％（ｎ＝１３），Ｔｋ３阳性细胞占２．０６±１．２５％（ｎ＝８），差别显著（Ｐ＜０．０５）。每毫升脐血两种抗体标记的细胞分别为９６．５６±５６．６４和２３１．４０±１６３．９３（Ｐ＜０．０５）。尽管ＨＰＣＡ－２阳性细胞与Ｔｋ３阳性细胞数量呈显著正相关（ｒ＝０．８７５，Ｐ＜０．０１），前者与ＣＦＵ－ＧＭ，ＢＦＵＥ，ＣＦＵ－Ｍｉｘ及集落总数ＣＦＵｓ均呈正相关，而后者仅与ＣＦＵ－ＧＭ，ＣＦＵｓ相关。研究提示在检测造血祖细胞时，用抗－ＨＰＣＡ－２－ＦＩＴＣ代替Ｔｋ３可降低假阳性，获得较好的ＯＤ（３４＋）细胞与ＣＦＵ间的线性关系。     

正常人体多毛部位皮肤雌激素受体的比较研究

应用直接荧光组化法对１０例正常男性头皮、眉部、胡须、腋部、阴部皮肤作比较研究。结果显示皮肤和附属器均有雌激素受体（ＥＲ）存在，而部位不同，含量各异。阻断对照：阻断率５０％～９０％。进一步证实皮肤是雌激素靶器官之一。     

Biotransformation of tipredane, a novel topical steroid, in mouse, rat, and human liver homogenates

The in vitro biotransformation pathways of 3H-tipredane (3H-TP) were studied. 3H-TP, at concentrations of 1 and 250 microM, was incubated with the 10,000g supernatant fraction of the liver homogenates of mice, rats, and one human. The incubation mixtures were deproteinated with methanol and, after removal of methanol by evaporation, extracted with dichloromethane. The dichloromethane extracts were then fractionated by HPLC. 3H-TP was extensively biotransformed by the liver homogenates of the three species studied; 17 metabolites were isolated and characterized by their retention times on HPLC compared to those of the reference standards. Fourteen metabolites were identified using MS and, for some, NMR spectroscopy. Three major biotransformation pathways of TP were identified: 1) sulfoxidation, 2) elimination of the alkylthio groups, and 3) hydroxylation of the steroid nucleus. Combinations of these processes and subsequent reactions resulted in the formation of numerous metabolites whose biological activities were significantly less than that of TP. The separation of local anti-inflammatory activity from systemic side effects observed for TP in animals and humans is most probably due to its metabolic inactivation, primarily in the liver.     

略论广东农村自学考试发展

高等教育自学考试是我国高等教育的重要组成部分 ,面向农村的自学考试事关我国“三农问题”、全民教育、加速实现“小康”的大局 ,其意义重大 ,任务艰巨。文章回顾了我国及广东省自学考试 2 0多年来的发展历程 ;分析了造成当前广东省农村自学考试面临困境的多种因素 ;探讨了加快广东省面向农村自学考试创新发展的途径。     

Factors associated with hypertension in Nigerian civil servants

BACKGROUND. Study of hypertension in segments of West African populations in transition toward Westernization may lead to better understanding of the high risk for hypertension among Westernized blacks. METHODS. Five hundred fifty-nine urban civil servants, ages 25-54, were recruited from six ministries of Bendel State, Nigeria. Blood pressure, physical measurements, urinary protein and glucose, fasting blood glucose, and demographic data were collected at the workplace. Subjects were classified as senior staff (professionals or administrators) or junior staff (non-administrators). RESULTS. Among 172 male senior staff, the age-adjusted rate of hypertension (diastolic blood pressure > or = 90 mm Hg, systolic blood pressure > or = 140 mm Hg, or on an antihypertensive medication) was 43% and occurrence rose dramatically from 21 to 63% across age groups 25-34 to 45-54, respectively. Among 266 male junior staff, the age-adjusted rate of hypertension was 23%, and occurrence did not rise with age. Logistic regression showed that body mass index (kg/m2), age, alcohol drinking, and being senior staff were all independently related to hypertension in men. On the other hand, the age-adjusted rate of hypertension in 121 women was 20% and was significantly related only to body mass index. CONCLUSION. Male urban civil servants appeared to have a risk for hypertension similar to that of U.S. black males. Age, body mass index, alcohol drinking, and other unidentified factors related to higher socioeconomic status were strong determinants of hypertension in this population.     

老年心房颤动病人华法林抗凝治疗的有效性安全性评价

目的　评价华法林用于老年心房颤动病人抗凝治疗的有效性、安全性。方法　选择符合本研究抗凝标准的132例老年心房颤动病人随机分为两组 ,华法林治疗组 (治疗组 ) 5 8例 ,给予华法林 3mg/ d开始 ,监测凝血酶原时间(PT)及国际标准化比值 (INR) ,7～ 15 d使 INR达到 1.8～ 2 .5范围内 ,以后每月查 1次 INR。若病人增加或减少药物有出血倾向时随时再测 INR。阿司匹林对照组 (对照组 ) 74例 ,给予阿司匹林 30 0 mg/ d,分 2次口服 ,密切随访。结果　治疗组 7～ 15 d,平均 (9.1± 2 .8) d,INR达 1.8～ 3.0 (平均 2 .1± 0 .13) ,其中 INR在 1.8～ 2 .5 (平均 1.92±0 .2 3)之间者占 92 .7% ,治疗组有 1例心瓣膜病人出现脑梗死 ,而对照组有 7例发生脑梗死 ,差异显著 ,其余不良反应率两组差异无显著性。结论　老年心房颤动病人选择华法林 2～ 3mg/ d时 ,加强服药后监测及各药物间的相互作用 ,使 INR保持在 1.8～ 2 .5之间是有效、安全的     

血小板第4因子对辐射损伤小鼠免疫功能的保护作用

目的：探讨血小板第4因子（plateletfactor4，PF4）对辐射损伤小鼠免疫系统的影响。方法：雄性BALB／c小鼠随机分为3组，第1组（单纯照射组）、第Ⅱ组（PF4保护组）、第Ⅲ组（正常对照组），第Ⅱ组在照射前26h及20h腹腔注射PF440μg／kg，全身一次性5Gy60Co—γ射线照射后瑞氏染色法计数外周血淋巴细胞百分数。胸腺、脾脏制成细胞悬液计数后，MTT比色法测定胸腺及脾细胞增殖能力。结果：第Ⅱ组小鼠外周血淋巴细胞百分数下降趋势较第1组明显减慢。胸腺、脾细胞计数，第Ⅱ组明显高于第1组。淋巴细胞增殖试验，第Ⅱ组与第1组相比，小鼠胸腺及脾细胞的增殖能力明显增强。结论：PF4对免疫系统有辐射保护作用，可明显增强辐射损伤小鼠的免疫功能。     

左旋多巴诱发异动症大鼠纹状体前强啡肽原基因与DARPP-32蛋白磷酸化的关系

目的研究左旋多巴诱发异动症(levodopa-induced dyskinesias,LID)大鼠纹状体内前强啡肽原(prodynorphin,PDyn)基因表达与DARPP-32蛋白磷酸化状态的变化,探讨LID时直接通路过度活化的机制。方法6-羟多巴胺(6-OHDA)大鼠模型应用左旋多巴治疗28 d诱发LID大鼠模型。采用原位杂交技术检测PDyn mRNA水平,逆转录-聚合酶链反应(RT-PCR)及免疫印迹技术检测LID大鼠纹状体内总DARPP-32的mRNA与蛋白表达及其Thr-34位点磷酸化水平。结果LID大鼠毁损侧PDyn mRNA表达(0.3662±0.0625)较对照组(0.2085±0.0573)及L-dopa治疗组(0.2235±0.0582)明显增高,差异有统计学意义(P<0.01)。LID大鼠毁损侧Thr-34位点磷酸化的DARPP-32蛋白水平(1075.2±103.3)较对照组(198.7±49.5)及L-dopa治疗组(213.9±58.9)明显增高,差异均有统计学意义(P<0.01)。结论DARPP-32蛋白的Thr-34位点的磷酸化水平的改变是LID时多巴胺D1受体介导的直接通路异常活化的关键因素之一。     

类风湿关节炎致自发性手指肌腱断裂的临床分析

目的探讨类风湿关节炎致自发性手指肌腱断裂的临床特点和治疗效果。方法对8例自发性手指肌腱断裂的患者（均有3～8年类风湿关节炎的病史），其中2例拇长伸肌腱断裂，采用示指固有伸肌腱转位修复；3例环、小指指伸肌腱断裂，采用环指指伸肌腱与中指指伸肌腱端侧编织缝合，示指固有伸肌腱移位修复小指固有伸肌腱；2例中、环、小指指伸肌腱断裂，采用冷冻异体指伸肌腱移植修复。1例拇长屈肌腱断裂，采用环指指浅屈肌腱转位修复。结果术后局部滑膜和肌腱组织送病理检查均显示为慢性炎症伴局灶性坏死。术后随访6个月～7年，未见肌腱再次断裂，优良率为100％。结论多根肌腱同时断裂是类风湿关节炎致自发性手指肌腱断裂的常见特点，手术采用肌腱转位或异体肌腱移植术，疗效确切。