The anxiolytic effects of the δ-opioid receptor agonist dalargin revealed in two behavioral tests in outbred albino rats were
blocked by naloxone. The effect of dalargin depended on the initial locomotor activity and was observed after central and
peripheral administration of the drug. The μ-opioid receptor agonist DAGO produced no behavioral effects. It is suggested
that the development of behavioral manifestations of anxiety and phobia is related to more than one opioid-dependent mechanism,
which can explain the peculiarities of the anxiolytic effect of dalargin.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 127, No. 2. pp. 211–214, February 1999 相似文献
Generation of protein immunogens is often a rate-limiting step in the production of monoclonal antibodies (Mabs). Expressing domains of proteins as fusions to the baculovirus surface glycoprotein gp64 displays foreign proteins on the surface of the virion. Antigen is produced by inserting a gene fragment in-frame between the signal sequence and the mature protein domain of the gp64 nucleotide sequence. This method allows immunization with whole virus, eliminating the need for purification of target antigens. Affinity-matured Mabs to the human nuclear receptors LXRbeta and FXR have been produced using baculovirus particles displaying gp64/nuclear receptor fusion proteins as the immunizing agent. Immunizations were performed directly with pelleted virus using the Repetitive Immunization Multiple Sites (RIMMS) immunization strategy for rapid Mab production. All Mabs were identified using insect cells infected with the immunizing virus. Characterization of these antibodies shows them to be class-switched and specific for LXRbeta or FXR. Additionally, high affinity antibodies that recognize gp64 and neutralize baculovirus infection of insect cells were isolated. Use of the recombinant baculovirus gp64 display system makes possible the production of Mabs once a partial DNA sequence is known. This allows the generation of antibodies prior to the isolation of purified protein, in turn providing antibodies to facilitate purification, characterization and immunolocalization of proteins. 相似文献
Opioids have long held a prominent role in the management of perioperative pain in adults and children. Published reports concerning the appropriate, and inappropriate, use of these medications in pediatric patients have appeared in various publications over the last 50 years. For this document, the Society for Pediatric Anesthesia appointed a taskforce to evaluate the available literature and formulate recommendations with respect to the most salient aspects of perioperative opioid administration in children. The recommendations are graded based on the strength of the available evidence, with consensus of the experts applied for those issues where evidence is not available. The goal of the recommendations was to address the most important issues concerning opioid administration to children after surgery, including appropriate assessment of pain, monitoring of patients on opioid therapy, opioid dosing considerations, side effects of opioid treatment, strategies for opioid delivery, and assessment of analgesic efficacy. Regular updates are planned with a re‐release of guidelines every 2 years. 相似文献
Introduction: US hospitals that admitted Ebola virus disease (EVD) patients mitigated risk by using point-or-care testing (POCT) for critical support in isolation units. Success proved unequivocally the need for POCT. Additionally, molecular diagnostics have been used to help stop new outbreaks, and even handheld diagnostic solutions are emerging.
Areas covered: This update of ‘Molecular detection and point-of-care testing in Ebola virus disease and other threats’ [Expert Reviews 2015;15(10):1249–1255], assesses the impact of EVD epidemics, documents insights from recent reviews, summarizes evolving POC molecular technologies, presents General Accountability Office (GAO) recommendations, identifies the role of POC Coordinators, and casts a vision for national POCT policies and guidelines. Factual updating comprised summarizing EVD outbreaks including 2017–2018, analyzing reviews and evidence-based publications since the 2014–2016 epidemic, and tabulating published technical and molecular diagnostics. New graphics illustrate POC error mitigation/risk reduction, a framework for national POCT policy and guidelines, modular adaptations for country-specific solutions, and a logic diagram for future progress embedding artificial intelligence.
Expert commentary: The USA is still not prepared for highly infectious diseases. Key is lack of community rapid response and resilience, which must be enhanced not via mechanisms distant, but instead by molecular diagnostics directly at critical points of need. 相似文献
Double‐hydrophilic block copolymers (DHBCs) of poly((4‐diethylamino)‐(E)‐stilbene)‐alt‐maleic acid)‐block‐acryloyl morpholine (DEASti‐alt‐MA)‐b‐ACMO), semirigid‐b‐flexible coil structures, form polyion complexes (PICs) in aqueous solutions. The pH and salt responsiveness of the PICs are studied using dynamic light scattering (DLS). PIC complex formation is dependent on block segment lengths and pH. The particle size increases as the pH approaches the IEP (isoelectric point) of the DHBC. The salt responsiveness is also measured by using DLS to monitor PIC size for four examples with different segment molar masses. These PICs exhibit an antipolyelectrolyte effect with increasing hydrodynamic diameter as ionic strength (NaCl) increases from 0.01 to 2.0 m . PICs are more stable at higher ionic strengths with higher ACMO molar mass.
