全文获取类型
收费全文 | 11310篇 |
免费 | 566篇 |
国内免费 | 48篇 |
专业分类
耳鼻咽喉 | 94篇 |
儿科学 | 181篇 |
妇产科学 | 107篇 |
基础医学 | 1639篇 |
口腔科学 | 201篇 |
临床医学 | 742篇 |
内科学 | 2839篇 |
皮肤病学 | 193篇 |
神经病学 | 789篇 |
特种医学 | 410篇 |
外科学 | 1876篇 |
综合类 | 42篇 |
预防医学 | 395篇 |
眼科学 | 237篇 |
药学 | 817篇 |
1篇 | |
中国医学 | 19篇 |
肿瘤学 | 1342篇 |
出版年
2023年 | 68篇 |
2022年 | 130篇 |
2021年 | 208篇 |
2020年 | 91篇 |
2019年 | 153篇 |
2018年 | 198篇 |
2017年 | 148篇 |
2016年 | 185篇 |
2015年 | 198篇 |
2014年 | 223篇 |
2013年 | 283篇 |
2012年 | 460篇 |
2011年 | 513篇 |
2010年 | 259篇 |
2009年 | 256篇 |
2008年 | 449篇 |
2007年 | 490篇 |
2006年 | 485篇 |
2005年 | 448篇 |
2004年 | 446篇 |
2003年 | 454篇 |
2002年 | 460篇 |
2001年 | 469篇 |
2000年 | 485篇 |
1999年 | 397篇 |
1998年 | 171篇 |
1997年 | 122篇 |
1996年 | 135篇 |
1995年 | 117篇 |
1994年 | 109篇 |
1993年 | 89篇 |
1992年 | 306篇 |
1991年 | 245篇 |
1990年 | 257篇 |
1989年 | 252篇 |
1988年 | 219篇 |
1987年 | 187篇 |
1986年 | 205篇 |
1985年 | 200篇 |
1984年 | 151篇 |
1983年 | 119篇 |
1982年 | 62篇 |
1979年 | 138篇 |
1978年 | 83篇 |
1977年 | 74篇 |
1975年 | 64篇 |
1974年 | 65篇 |
1972年 | 58篇 |
1971年 | 64篇 |
1969年 | 57篇 |
排序方式: 共有10000条查询结果,搜索用时 9 毫秒
101.
102.
Patricia Chastagner Jean-Louis Moreau Yannick Jacques Toshiyuki Tanaka Masayuki Miyasaka Motonari Kondo Kazuo Sugamura Jacques Thze 《European journal of immunology》1996,26(1):201-206
An interleukin (IL)-4 dependant mouse T cell clone 8.2 derived from an IL-2-dependent T cell line was characterized. As measured by flow cytometric analysis and Northern blotting, it expresses IL-2 receptor β (IL-2Rβ) and γ (IL-2Rγ) chains, but has lost expression of IL-2 receptor α chain (IL-2Rα). To investigate the properties of the mouse IL-2Rβγ complex and the role of IL-2Rα gene expression, this clone was further studied. T cell clone 8.2 has lost the capacity to bind 125I-labeled human IL-2 under experimental conditions able to detect intermediate-affinity IL-2R in human cells. Mouse IL-2 is unable to block the binding of mAb TMβ1 to 8.2 cells. Under the same experimental conditions, mouse IL-2 blocks the binding of TMβ1 to C30-1 cells expressing the IL-2αβγ complex. Since TMβ1 recognizes an epitope related to the IL-2 binding site of IL-2Rβ, these results can be taken as a demonstration that mouse IL-2Rβγ does not bind mouse IL-2. Furthermore, T cell clone 8.2 does not proliferate in response to recombinant mouse or human IL-2. On the other hand, T cell transfectant lines expressing heterospecific receptors made of the human IL-2Rβ and mouse IL-2Rγ chains bind 125I-labeled human IL-2 and proliferate in response to IL-2. This establishes the difference between mouse and human IL-2Rβ chains. Transfection of T cell clone 8.2 with human IL-2Rα genes restores their capacity to proliferate in response to IL-2. In addition, all transfectants grown in IL-2 express the endogeneous mouse IL-2Rα chain. When grown in IL-4, the endogeneous mouse IL-2Rα gene remains silent in all these transfectants. These results show that, contrary to the human, the mouse does not express an intermediate-affinity IL-2R. Expression of the IL-2Rα gene is therefore required for the formation of the functional IL-2R in mice. 相似文献
103.
Characterization and expression of cDNA encoding coproporphyrinogen oxidase from a patient with hereditary coproporphyria 总被引:2,自引:1,他引:2
Fujita Hiroyoshi; Kondo Masao; Taketani Shigeru; Nomura Nakao; Furuyama Kazumichi; Akagi Relko; Nagai Tadashi; Terajima Masanori; Galbraith Richard A.; Sassa Shigeru 《Human molecular genetics》1994,3(10):1807-1810
Hereditary coproporphyria (HCP) is an acute hepatic porphyriawith autosomal dominant inheritance, but with a variable degreeof clinical expression. Molecular cloning, sequencing and expressionof the defective gene for coproporphyrinogen oxidase (CPO) ina patient with HCP were carried out. Enzyme assays revealedthat CPO activity in EBV-transformed lymphoblastoid cells fromthe proband and one of her sisters was 相似文献
104.
105.
Shibahara M Nishida K Asahara H Yoshikawa T Mitani S Kondo Y Inoue H 《Acta medica Okayama》2000,54(2):67-74
We investigated the presence of osteocyte apoptosis in the necrotic trabeculae of the femoral head of spontaneously hypertensive rat (SHR) using the in situ nick end labeling (TUNEL) method and transmission electron microscopy. The occurrence of osteonecrosis and ossification disturbance was significantly higher in SHR compared with Wistar Kyoto (WKY) rats, and Wistar (WT) rats used as control animals (P < 0.01). A high population of TUNEL positive osteocytes was detected mainly in 10- and 15-week-old SHRs. Sectioned examination of the femoral head of SHRs and WKY rats by electron microscopy revealed apoptotic cell appearances such as aggregation of chromatin particles and lipid formation. In contrast, a positive reaction was significantly lower in osteocytes in the femoral heads of WT rats (P < 0.01). Our results indicate that apoptosis forms an important component of the global pathologic process affecting the femoral head of SHR, which leads to osteonecrosis in this region. 相似文献
106.
107.
Mitsuo Masuno Jun-Ichi Asano Kanji Yasuda Tomio Kondo Tadao Orii 《American journal of medical genetics. Part A》1993,45(1):65-67
We report on a 19-month-old girl with a derivative chromosome 9 and a recombinant chromosome 12 resulting from a maternal balanced complex rearrangement involving chromosomes 8, 9, and 12. The karyotype of the phenotypically normal mother was 46,XX,t(8;12) (9;12) (8qter→8p23::12q12→12q15::9q32→9qter;9pter→9q32::12q15→12qter;12pter→12q12::8p23→8pter). The child's karyotype was 46,XX,?9,?12, +der(9) (9pter→9q32::12q15→12qter),+rec(12) (12pter→12q15::9q32→9qter) mat. The child had severe growth retardation, minor anomalies including trigonocephaly, hypertelorism, broad nasal root, apparently low-set and posteriorly angulated ears, triangular face, pectus carinatum, clinodactyly of fifth fingers, and almost normal psychomotor development. To the best of our knowledge, there have been only 3 previous reports of recombination derived from parental complex chromosome rearrangements. In the recombination products, the chromosomes were apparently balanced and the offspring had no clinical abnormalities. The present case exhibited abnormalities and may have a submicroscopic aberration of 12q arising from crossing over during maternal meiotic pairing, although her chromosomes appeared to be balanced. © 1993 Wiley-Liss, Inc. 相似文献
108.
109.
作者探讨了鸡蛋过敏特应性皮炎(AD)患儿外周血单个核细胞(PBMCs)对卵白蛋白(OA)刺激的增殖反应并观察了酮替酚(KF)对此反应的影响.结果提示AD患儿的PBMCs增殖反应显著高于健康儿童;KF可剂量依赖性地抑制鸡蛋过敏AD患儿PBMCs对OA刺激的增殖反应,且这一效应可通过KF抑制T细胞的作用而得以实现.KF对植物血凝素(PHA)和破伤风类毒素(TT)诱导的PBMCs增殖反应无抑制作用.这说明KF只抑制食物过敏AD患者PBMCs对食物抗原的特异性增殖反应. 相似文献
110.