Fluorescent Substances and High Molecular Weight Protein Aggregates Formed in Rat Heart Mitochondria upon Doxorubicin-induced Lipid Peroxidation

A rat heart mitochondrial suspension was incubated with doxorubicin, FeCl₃ and NADH. Fluorescent substances and high molecular weight protein aggregates were observed in the mitochondrial membranes upon the formation of thiobarbituric acid-reactive substances. Since both fluorescent substances and high molecular weight protein aggregates are retained in mitochondrial membranes, they can be of use in the clarification of the site of doxorubicin-induced lipid peroxidation.     

Colonic Lipoma Removed by Endoscopic Polypectomy:—A Report of Three Cases—

We report on three cases of colonic lipoma removed by endoscopic polypectomy. Patient 1 was a 46-year-old female, Patient 2 a 63-year-old male and Patient 3 a 76-year-old female, with their afflicted regions being the; ascending colon, ascending colon and transverse colon, respectively. All the three lipomas were pedunculated and smooth on the surface, with the subrnucosal figure presenting yellowish or reddish in color. In Patient 1 and Patient 2, both tumors were totally removed, and in Patient 3, a “half resection” of the polyp was performed by endoscopic polypectomy. The resected specimens were 1.5 cm, 2.6 cm and 1.0 cm, respectively, in size at their maximum diameter and soft tumors with uniformly yellow cut-surfaces. All were histopathologically diagnosed as being “lipoma”. Because the colonic lipoma is benign, an endoscopic polypectomy should be tried as the treatment of choice in selected patients.     

Methylmethacrylate bone cement does not release histamine in patients undergoing prosthetic replacement of the femoral head

This study was designed to see if methyl methacrylate monomerbone cement released his tamine in 13 patients undergoing totalhip replacement surgery with a cemented prosthesis, comparedwith seven control patients receiving a cementless porous-coatedprosthesis. Blood samples for plasma concentrations of histaminewere obtained before the start of anaesthesia, immediately beforeinsertion of methylmethacrylate bone cement into the shaft ofthe femur in the cemented fixation group or before insertionof the femoral component of the prosthesis in the cementlessfixation group, and 15, 30 and 60 min after the start of implantationof the prosthesis. In both groups, changes in plasma histaminedid not differ significantly from baseline before implantationof cement. There were no significant differences between groups.We conclude that methyl-methacrylate bone cement does not releasehis tamine during total hip replacement surgery.     

Development of an Imaging System Using Fluorescent Labeling Substances Excited by Infrared Rays

Abstract: An indocyanine green (ICG) derivative (ICG N-hydroxy sulfosuc-cinimide ester; ICG-sulfo-OSu) has been developed as an antibody labeling substance suitable for vital immunohistochemical staining. However, an appropriate fluorescence imaging system for ICG-sulfo-OSu using infrared rays has not as yet been reported. Therefore, we developed such a system. The absorption maxima of ICG and ICG-sulfo-OSu in buffer solution are both at 795 nm, the excitation maxima of their fluorescence spectra in buffer solution at 768 nm, and their emission maxima at 807 nm. An imaging system using an excitation filter with transmission at 710-790 nm and a barrier filter with transmission at 810-920 nm was constructed, and fluorescent images of ICG-sulfo-OSu labeled anti-epithelial membrane antigen (EMA) antibody were obtained with this system. Thus, vital immunohistochemical staining of microcancers under infrared ray excitation should now be possible, by exciting an ICG-sulfo-OSu labeled antibody specific to the tumor cells with infrared rays, using this imaging system.     

A side reaction in solid phase synthesis Insertion of glycine residues into peptide chains via Nim→ Nα transfer

In solid-phase peptide synthesis using N^α-Boc-N^im-tosyl-histidine (Boc-His(Tos)), byproducts having extra Gly residues in the peptide chain were observed at a high rate. When a Boc-amino acid such as Asn was incorporated after assembly of Boc-His(Tos), the N^im-tosyl group was partially or fully cleaved by an activating agent, 1-hydroxybenzotriazole. In the successive coupling reactions, Boc-Gly was incorporated into the free N^im ring as well as the α-amino function, and the N^im-Gly was then transferred to the α-amino group of Gly of the peptide chain after removal of these Boc groups to give extra Gly residues at the position of Gly. This was observed in only the coupling reaction with Boc-Gly and could be circumvented using a more stable N^im protecting group for His, such as a dinitrophenyl group.     

Oil-Depot Type Bleomycin in the Treatment of Malignant Lymphoma in Comparison with Regular Bleomycin

Oil-depot type bleomycin was originally intended to take advantageof not only concentration-dependent action, but also the time-dependentaction of bleomycin to obtain more efficient tumor cell kill.In this study, the effects of oil-depot type bleomycin and regularbleomycin on 81 patients with malignant lymphoma were compared.Both oil-depot type bleomycin and regular bleomycin were equallyeffective against Hodgkin's disease, with a complete remissionrate of 60% (6/10) and 54.5% (6/11), respectively. Non-Hodgkin's lymphoma in advanced stages responded better tooil-depot type bleomycin [complete remission (CR); 35.2% (6/17)]than to regular bleomycin [CR: 10.5% (2/19)]. In addition, thepatients were more responsive to smaller doses of oil-depottype bleomycin than regular bleomycin. Eleven out of 12 (91.6%)patients who responded to oil-depot type bleomycin, went intocomplete remission before receiving 45 mg in total dosage ofbleomycin, while five out of 10 (50%) patients who respondedto regular bleomycin reached complete remission after 60 mgin total dosage was administered. The toxic manifestations of oil-depot type bleomycin were almostthe same in quality and quantity as regular bleomycin. However,the average total dose of oil-depot type bleomycin used wasalmost half of that of regular bleomycin. ^* Present address: Cancer Chemotherapy Center, Cancer ResearchInstitute, Tokyo, Japan. ^** Present address: National Nagoya Hospital, Nagoya, Japan.     

中药三七质量评价半微量方法及在三七连作障碍样品分析中的应用

该研究旨在建立三七质量评价半微量方法并应用于三七连作障碍样品分析。采用100倍量水饱和正丁醇超声提取0.1 g三七样品,所建立的半微量提取方法相比于甲醇常量提取法对5个主要皂苷类成分及三七皂苷K和Fa提取效率高9.6%~20.6%,且重复性良好,RSD2.5%。LC-MS-IT-TOF技术指认指纹图谱16个皂苷类成分特征峰,包括8个人参二醇型皂苷和8个人参三醇型皂苷。利用HPLC指纹图谱相似度评价及对特征峰峰面积提取可进行三七的质量分析。应用所建立的方法对人工干预克服连作障碍三七样品质量评价的结果表明:指纹图谱相似度、三七皂苷Fa的相对含量及三七皂苷K与三七皂苷Fa的比值(N-K/Fa值)均可作为评价连作地三七质量恢复程度的指标,其中N-K/Fa值同样适用于不同生长年限三七样品的区分。正常(无连作栽培)组样品三七皂苷K与三七皂苷Fa的峰面积比值为0.45~1.33;连作组样品个头小于正常组,指纹图谱与正常组相似度均低于0.87,三七皂苷Fa的峰面积显著低于正常组,N-K/Fa值显著高于正常组,为2.35~4.74,与正常组三七样品质量存在差异。人工干预一组的样品与正常组相似度均高于0.87,且16个特征峰的峰面积和分布模式均与正常组无显著差异,N-K/Fa值为0.42~2.06。人工干预二组的样品除重量较小的样品外,其余均与正常组指纹图谱相似度较高,三七皂苷K显著高于正常组,N-K/Fa值为0.96~6.16。连作障碍经人工干预后三七的质量均有不同程度的恢复和提高,其中人工干预一组的三七质量恢复情况优于人工干预二组。该研究建立的三七质量评价半微量方法,采用指纹图谱及峰面积比值作为评价指标可避免含量测定的繁琐及昂贵,为连作障碍三七样品的质量分析提供了简便、快速和准确的评价方法,且可应用于三七不同年限等样品的质量评价。     

Prognostic Factors and Therapeutic Results in Multiple Myeloma

Prognostic factors in 68 consecutive patients with myeloma treatedat the National Cancer Center Hospital from 1962 to 1984 wereanalyzed. Median survival time from onset was 100 months forstage I, 72 months for stage II, and 26 months for stage IIIof the Durie and Salmon's clinical staging system. It was 55months in patients with normal renal function and 18 monthsin those with abnormal renal function. All early deaths occurredin patients with stage III disease. Hemoglobin level, bone lyticlegions and presence of Bence Jones protein were also significantprognostic factors. On the other hand, heavy chain as well aslight chain subtypes of monoclonal immunoglobulin (M-component)and M-component production rate did not influence the survivalof myeloma patients. The analysis of chemotherapeutic responsesand survival curves according to the chemotherapy used in thisstudy (alkylating agent vs Vinca-alkaloid plus alkylating agent)did not disclose any significant difference between the twogroups. The overall response rate was 67%. The survival timefrom the initial chemotherapy of responding patients was significantlylonger than that of nonresponders.