深圳地区变应性鼻炎变应原分析

目的了解深圳地区变应性鼻炎的常见变应原。方法对855例疑似变应性鼻炎的患者进行皮肤点刺试验，测定致敏变应原。结果855例疑似变应性鼻炎患者中578例（67．6％）有阳性反应，单一变应原阳性51例，2种以上变应原阳性527例。以吸人性变应原为主，其中65例有哮喘病史。578例阳性病例中屋尘螨阳性率最高，为520例（90％），其次是粉尘螨488例（84．4％）。结论应针对深圳地区变应性鼻炎的常见变应原（屋尘螨、粉尘螨），做好变应原检测工作，提高检测准确率。     

HPLC-RI法测定银杏叶提取物中4种内酯的含量

目的:建立高效液相色谱法测定银杏叶提取物中4种内酯(银杏内酯 A、银杏内酯 B、银杏内酯 C、白果内酯)的含量。方法:采用 Symmetryshield 型 C_(18)色谱柱(3.9 mm×150 mm,5 μm),以甲醇-水(30:70)为流动相,流速为1.0 mL·min~~(-1),用外标法测定,示差折光检测器检测。结果:4种内酯浓度在0.02～0.24 mg·mL~(-1)范围内,与色谱峰面积呈良好线性关系,相关系数在0.9984～0.9998之间,检测限在0.025～0.035 μg之间,回收率在99.0%～100.2%之间。结论:本方法简单、准确、可靠、灵敏,适用于实验室和企业常规质量控制。     

辛伐他汀对高血压并发2型糖尿病患者颈动脉粥样硬化的影响

目的:探讨辛伐他汀对高血压并发2型糖尿病(T2DM)患者颈动脉粥样硬化的影响.方法:并发T2DM的原发性高血压患者92例,随机分为2组:辛伐他汀组50例,常规治疗组42例.2组均接受常规降压、降糖、抗血小板聚集治疗,辛伐他汀组加用辛伐他汀20 mg,每晚1次.分别于入院时及治疗6个月后检测血浆高敏C反应蛋白(hsCRP)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)及高密度脂蛋白胆固醇(HDL-C),彩色多普勒超声检测记录颈动脉内膜-中膜厚度(IMT),并计算颈动脉斑块积分.结果:常规治疗组治疗前后血浆TC、TG、LDL-C、HDL-C及hsCRP水平差异无统计学意义(P>0.05),治疗后IMT、斑块积分上升(P<0.05).辛伐他汀组治疗后除HDL-C上升外,其余血脂指标、hsCRP、IMT及颈动脉斑块积分均较治疗前下降(P<0.01),且hsCRP与IMT及颈动脉斑块积分成正相关(r分别为0.85、0.79, P<0.05).结论:辛伐他汀通过调节血脂、抗炎作用,改善高血压并发T2DM患者颈动脉粥样硬化程度.     

紫癜风(过敏性紫癜)诊治方案应用分析

目的 通过对国家中医药管理局重点专科血液病协作组紫癜风(过敏性紫癜)协作分组各单位提供的紫癜风诊疗方案进行梳理,得出各单位的共识.方法 采用定性分析方法,对紫癜风的临床疗效、中医治疗方法、治疗难点等进行系统分析.结果与结论 对中医治疗紫癜风的难点提出了应对思路.分析整理后的经过多名专家认可的总结诊疗方案可以在全国推广使用.     

Ku86通过调控TOP1和COPS5影响上皮性卵巢癌细胞对于顺铂的化疗敏感性

背景与目的:卵巢癌是妇科恶性肿瘤中发病率排名第3、致死率排名第1的疾病.卵巢癌的预后很差,这是由于大部分患者确诊时已处于晚期并且对铂类药物化疗易耐药,异常的DNA修复是导致铂类耐药的重要原因,针对性地干扰DNA损伤修复相关分子可能是提高铂类药物化疗敏感性的新手段.Ku86是参与非同源末端连接(non-homologou...     

'Baby Check' in the Inner City--Use and Value to Parents

‘Baby Check’, an information booklet designed toaid assessment of acutely ill babies, was given to a group ofsocioeconomically disadvantaged families from one inner citygeneral practice. Parents' perceptions and use of the bookletwere explored in a qualitative study based on audiotaped interviewswith 42 parents of 34 babies under 6 months old. Most families(26) used Baby Check spontaneously. Parents found that BabyCheck was helpful, reduced anxiety and increased confidencecoping with illness, seeking medical advice and communicatingwith doctors. Parents experienced some difficulties, e.g. theyhad concerns about a check for rectal temperature and they soughtmore information and advice about common illnesses. Parentsvalued and were empowered by access to ‘medical’information. Further evaluation and consultation with parentsis required to ensure that information is relevant to theirneeds.     

DISTRIBUTION OF CATALASE IN RAT BRAIN: AMINERGIC NEURONS AS POSSIBLE TARGETS FOR ETHANOL EFFECTS

Ethanol is metabolized at a slow but measurable rate in rodentbrain Recent studies indicate that this process is mediatedmainly by catalase. The spatial distribution of this enzymein different brain structures is poorly known. To explore possiblelocal imbalances between the production and elimination of ethanol-derivedacetaldehyde. we investigated the regional and cellular distributionof catalase. histo- and immunohistochcmically. using serialcryostat sections from male Wistar rats Compared to the strongperoxisomal staining seen in liver, brain catalase stainingwas weak and was not immunologically detected with an anti-sheepbovine catalase antibody Activity was observed only in microperoxisomes.mainly in penkaryons of aminergic neurons, in the known groupsot adrenergic. noradrenergic and serotonergic neurons of thebrain stem. Little peroxisomal staining was seen in other typesof brain structures. This result contrasted to that of aldehydedehydrogenase. which we previously observed to be widely distributedin brain structures, but with low activity in penkaryons ofaminergic (especially catecholaminergic) neurons, as comparedto cholinergic neurons. Our data indicate that catalase-mcdiatcdoxidation of ethanol to acetaldehydc lakes place mainly in aminergicneurons, which seem to have a limited capacity for the subsequentremoval via aldehyde dehydrogenase This suggests that locallyproduced acetaldehyde could mediate CNS effects of ethanol inthese structures.