Age‐group‐specific reference intervals for anti‐Müllerian hormone and its diagnostic performance for polycystic ovary syndrome in a Korean population

BackgroundWe established age‐group‐specific reference intervals for serum anti‐Müllerian hormone (AMH) levels in a Korean population and investigated the effectiveness of AMH assay for polycystic ovary syndrome (PCOS) diagnosis.MethodsWe analyzed serum levels of AMH, follicle‐stimulating hormone (FSH), and luteinizing hormone (LH) from 1540 Korean women. Subjects were divided into three groups: healthy, benign gynecologic diseases, and PCOS. Age‐group‐specific reference intervals and AMH diagnostic performance were estimated.ResultsThe PCOS group had a median AMH level of 7.0 µg/L, which was higher than for the healthy (1.8 µg/L) and the benign gynecologic diseases (2.7 µg/L) groups. The upper 97.5% reference limits for age groups 12–20 years, 21–34 years, and 35–46 years were 13.2 µg/L, 15.8 µg/L, and 6.6 µg/L, respectively. The area under the curve (AUC) values to estimate AMH ability to discriminate PCOS from healthy women for each age group were 0.741, 0.785, and 0.789, respectively. AUCs for LH/FSH were 0.719, 0.672, and 0.590.ConclusionsThe better diagnostic ability of AMH over LH/FSH in women of late childbearing ages indicates that age and other clinical characteristics should be considered when interpreting these test results.     

合肥市社区医务人员食源性疾病认知及临床诊断能力调查

目的 调查合肥市社区医务人员食源性疾病知信行及其临床诊断能力并分析相关因素,为疾病预防控制机构和街道社区卫生服务中心内部的培训工作提供理论依据。方法 采用整群随机抽样的方法抽取41家街道社区卫生服务中心,选取929名医务人员进行问卷调查,采用单因素分析和多因素logistic回归模型分析医务人员基本特征对食源性疾病认知及其临床诊断能力的影响。结果 社区医务人员知识、态度、行为和临床情景模拟诊断的得分分别是（8.38±2.92）、（7.18±1.64）、（9.51±3.41）、（5.49±2.54）分。多因素分析表明科室是食源性疾病知识及行为的影响因素（P< 0.05）,是否参加培训是态度及行为的影响因素（P< 0.05）。结论 合肥市社区医务人员对食源性疾病的认知和诊断水平有待加强,培训应区别不同科室有针对性开展。     

A comparison of therapeutic efficacy of a melamine foam sponge and conventional cotton wool bud in the cryotherapy of viral warts: a paired comparison study

Many therapies have been studied for the treatment of viral warts, but none are uniformly effective. The aim of this study was to compare the efficacy of conventional cotton wool bud with that of melamine foam sponge as applicators in the treatment of warts with liquid nitrogen. A paired comparison study was conducted in 27 patients with at least two similar clinical types of warts. Similar clinical lesions were allocated to have liquid nitrogen applied with cotton wool bud or melamine foam sponge. Mean size reduction rate after the first treatment was 30.5% in the cotton wool bud group and 59.1% in the melamine foam sponge group. Mean number of total treatments was 3.05 in the cotton wool bud group and 2.29 in the melamine foam sponge group. Melamine foam sponge was found to be more effective in comparison to conventional cryotherapy with cotton wool bud.     

老年髋部骨折病人心理护理与功能康复护理研究

[目的]探讨对老年髋部骨折病人实施心理护理、功能康复护理的效果。[方法]将83例行手术治疗的老年（年龄≥60岁）髋部骨折病人分为观察组和对照组,对照组行常规护理,观察组在常规护理的基础上给予心理护理、功能康复护理。[结果]出院时两组病人焦虑（SAS）得分比较,差异有统计学意义（P〈0.05）。术后对两组病人随访12个月,观察组显效率明显高于对照组（P〈0.05）。[结论]对老年髋部骨折病人进行心理护理、功能康复护理,病人治疗期间焦虑情绪明显改善、远期疗效好。     

多元智能理论在护理临床实习教学中的应用研究

目的探讨护理临床实习教学中应用多元智能理论的效果。方法将我校护理学院在附院实习的2008届、2009届护生分为两组,其中,2008届为对照组,2009届为实验组。实验组采用传统教学法和以多元智能理论为指导的教学模式;对照组采用传统教学方法。教学评价采用考试和问卷调查。结果实验组与对照组学生理论考试成绩比较,差异无显著意义(P0.05);技能操作考试、综合能力考核成绩比较,差异有极显著意义(P0.01);问卷调查结果 ,认为该教学模式很好和较好的学生为82.35%。结论多元智能理论应用于护理临床实习教学,提高了教学效果,有利于学生综合素质的提高。为了更客观的评价教学效果,真实反映学生的智能水平,需建立多元化的护理临床实习教学评价新体系。     

我院1998年～1999年中成药用药分析

目的了解我院中成药的应用情况。方法调查我院1998年～1999年中成药的年销售金额,采用DDDs分析方法和药物经济学方法,分析中成药的销售量、用药频度、日用药金额。结果1998年～1999年我院中成药的销售金额和用药频度呈上升趋势。结论我院中成药的应用基本合理,价廉且疗效确切的中成药在临床应用中占优势。     

hsa_circ_0000285 facilitates thyroid cancer progression by regulating miR‐127‐5p/CDH2

Thyroid cancer (THCA) is a leading endocrine cancer and becomes the fifth most commonly diagnosed malignancy in females. It is confirmed that circular RNAs (circRNAs) perform regulatory potencies in the pathological progress of THCA. Our purpose was to certify the trait of hsa_circ_0000285 (circ_0000285) and investigate its modulatory mechanism in THCA progression. We identified the expression profile of hsa_circ_0000285 in THCA by conducting qRT‐PCR assay. Therewith, the potential of hsa_circ_0000285 in THCA development was determined with a set of functional experiments, including CCK‐8, wound healing assay, Western blot, and xenograft model. The molecular mechanism underlying hsa_circ_0000285 was investigated with bioinformatic analysis, RIP and dual‐luciferase reporter experiments. As opposed to normal samples and cells, hsa_circ_0000285 level was overtly increased in THCA specimens and cells. The downregulation of hsa_circ_0000285 weakened the proliferative and migratory capacity of THCA cells and promoted cell apoptosis. In addition, hsa_circ_0000285 silence suppressed the tumor growth of xenograft model mice in vivo. Notably, we demonstrated that hsa_circ_0000285 might target miR‐127‐5p/CDH2 axis in THCA. Afterward, our findings manifested that miR‐127‐5p attenuation blocked the function of hsa_circ_0000285 depletion in THCA cells. In the final step, CDH2 was proven to mediate the repressive potency of miR‐127‐5p in the malignant behaviors of THCA. Mechanistically, hsa_circ_0000285 induced the development of THCA via functioning as a competing endogenous RNA (ceRNA) of miR‐127‐5p to enhance CDH2 expression, which provided a new perspective for THCA therapy.     

RAD51基因在结直肠癌中的表达及与预后的关系

目的 探讨RAD51基因在结直肠癌（CRC）中的表达及其与临床病理特征和预后的关系。方法 收集72例CRC患者的病理标本（CRC组,n=72）,以及经病理证实无癌细胞存在、距肿瘤边缘>3 cm的肠黏膜组织（癌旁对照组,n=72）。采用qRT-PCR和Western blotting技术对CRC与癌旁组织的RAD51 mRNA和蛋白表达水平进行检测。影响CRC患者预后的因素采用Cox比例风险模型分析。预后分析采用Kaplan-Meier和Log-rank法。结果 CRC组RAD51 mRNA和蛋白相对表达量均高于癌旁对照组,差异有统计学意义（P<0.05）。CRC组织中RAD51表达水平与TNM分期有关（P<0.05）。RAD51高表达是影响CRC患者5年无瘤生存率和5年总体生存率的危险因素（P<0.05）。RAD51低表达的CRC患者5年总体生存率和5年无瘤生存率均高于RAD51高表达患者（P<0.05）。结论 RAD51在CRC组织中高表达,与TNM分期有关。RAD51高表达的CRC患者预后差,RAD51可能是临床上预测CRC患者预后的一个潜在指标。     

硬膜外麻醉对老年糖尿病患者血液流变学和血浆胰岛素及血糖水平的影响

目的 观察对比硬膜外麻醉对老年和非老年糖尿病患者围手术期间血液流变学、血浆胰岛素及血糖水平的影响,旨在为糖尿病患者手术时选择效果确切且安全的麻醉方式提供参考.方法 选取2016年1月至2017年2月期间120例择期手术的2型糖尿病择期手术患者为研究对象,根据年龄分为老年组和非老年组,均行硬膜外麻醉,对比分析两组患者于麻醉前(T0)、麻醉后30 min(T1)、手术结束时(T2)、术后30 min(T3)、术后24h (T4)6个不同时刻血液流变学指标[全血高切黏度、全血低切黏度、全血高切还原黏度、全血低切还原黏度]、血浆胰岛素、血糖水平变化情况.结果 两组患者T1-4血液流变学指标全血高切黏度、全血低切黏度、全血高切还原黏度、全血低切还原黏度水平均低于麻醉前(均P＜ 0.05),T2时降至最低水平,随后逐渐恢复,但尚未恢复至麻醉前水平,T4时各项水平均明显高于T2时水平(均P＜ 0.05),老年组患者T2和T4时刻各项指标均高于非老年组,除T2时刻全血高切黏度水平比较,P＞0.05,余均P＜0.05;两组患者血糖和血浆胰岛素水平呈逐渐增高趋势(P＜0.01),T1-4时水平均明显高于T0时水平,T2-4时水平均明显高于T1时水平(均P＜0.05),老年组患者T1-4时血浆胰岛素水平虽高于非老年组,但差异无统计学意义(P＞0.05),老年组患者T1-4时血糖水平均明显高于非老年组(均P＜ 0.05).结论 硬膜外麻醉可控制并减轻老年糖尿病患者应激反应,减轻胰岛素抵抗,维持血液流变学和糖代谢平稳,预防心血管事件等麻醉意外的发生.     

某三甲医院实施抗菌药物临床应用专项整治前后应用情况比较

目的 评价专项整治活动开展前后抗菌药物临床应用情况.方法 收集该院抗菌药物专项整治前,即2010年1月至2011年6月抗菌药物使用相关数据;与整治后2011年7月至2013年8月抗菌药物使用相关数据进行统计分析.结果 （1）实施专项整治后,该院抗菌药物使用日趋合理.监测指标中,住院患者抗菌药物使用率、抗菌药物使用强度、Ⅰ类切口抗菌药物预防使用率、外科手术预防使用抗菌药物时间在0.5～2h符合率及Ⅰ类切口手术预防用药疗程符合率整治前后差异有统计学意义（P＜0.05）.（2）该院铜绿假单胞菌和金黄色葡萄球菌耐药率在抗菌药物专项整治前后,经W检验差异有统计学意义（P＜0.05）.结论 抗菌药物专项整治非常有必要,通过专项整治,抗菌药物的使用更加合理.