1987～2001年同济医院门诊性传播疾病患者的临床分析

目的 对同济医院门诊1987—2001年性传播疾病(STDs)疫情资料进行临床分析，旨在为防治提供依据。方法 对确诊为STDs的患者进行流行病学调查，进行统计学处理。结果 近10年内累计报告STDs l2058例，病例、病种均增长。衣原体感染、支原体感染、梅毒、艾滋病和生殖器疱疹持续上升，淋病稳定下降。1987—1989年、1994—1996年和1999—2001年男女患者性别之比分别为5．59：1、1：2．45和1：1．04。平均年龄31．5岁，20—29岁和30—39岁年龄组分别占STDs发病总人数的42．62％和40．29％。结论 STDs发病总体上升，第二代性病是流行趋势；男女性别比差距逐年缩小；20—39岁性活跃年龄人群居主导地位。     

The usefulness of neutrophil CD64 expression in the diagnosis of local infection in patients with rheumatoid arthritis in daily practice

Background  

The diagnosis of local infection in patients with rheumatoid arthritis (RA) is frequently difficult because clinical signs and symptoms and laboratory test results of local infection are also observed in arthritis of active RA. The need for a specific marker of infection is high in RA patients. The usefulness of neutrophil CD64 expression (CD64) to diagnose local musculoskeletal infection (local infection) and discriminate local infection from RA-related inflammation in RA patients was examined.     

Thermal degradation characteristics and antioxidant activity of fructose solution with heating temperature and time

This study investigated the thermal degradation characteristics of fructose. A 20% fructose solution was heated to 110-150°C for 1-5 hours. Chromaticity, pH, organic acid, 5-hydroxymethylfurfural (HMF), fructose content, electron-donating ability (EDA) (as a percentage), and ascorbic acid (AA) equivalent antioxidant capacity (AEAC) of heated fructose solutions were evaluated. With increasing heating temperature and time, the L-value decreased, and the a- and b-values increased to 120°C for 4 hours and 2 hours, respectively, and then decreased thereafter; however, total color difference increased. The pH and fructose content decreased. Organic acids, such as formic acid, lactic acid, and levulinic acid, and HMF content increased with increasing heating temperature and time. Antioxidant activity increased with increasing heating temperature and time (no antioxidant activity in 20% fructose solution). The EDAs after heating at 130°C for 3 and 4 hours were 17.21% and 31.73%, respectively, and the highest antioxidant activity was 94.57% (150°C for 4 hours). The AEAC was 1.71?mg of AA eq/g before heating and 24.43?mg of AA eq/g after heating at 150°C for 4 hours. These results suggest that heat treatment may be a useful method for increasing the antioxidant activity of fluid foodstuffs such as fruit juices and fructose-containing foods.     

Fully biodegradable and cationic poly(amino oxalate) particles for the treatment of acetaminophen-induced acute liver failure

Acute inflammatory diseases are one of major causes of death in the world and there is great need for developing drug delivery systems that can target drugs to macrophages and enhance their therapeutic efficacy. Poly(amino oxalate) (PAOX) is a new family of fully biodegradable polymer that possesses tertiary amine groups in its backbone and has rapid hydrolytic degradation. In this study, we developed PAOX particles as drug delivery systems for treating acute liver failure (ALF) by taking the advantages of the natural propensity of particulate drug delivery systems to localize to the mononuclear phagocyte system, particularly to liver macrophages. PAOX particles showed a fast drug release kinetics and excellent biocompatibility in vitro and in vivo. A majority of PAOX particles were accumulated in liver, providing a rational strategy for effective treatment of ALF. A mouse model of acetaminophen (APAP)-induced ALF was used to evaluate the potential of PAOX particles using pentoxifylline (PTX) as a model drug. Treatment of PTX-loaded PAOX particles significantly reduced the activity of alanine transaminase (ALT) and inhibited hepatic cell damages in APAP-intoxicated mice. The high therapeutic efficacy of PTX-loaded PAOX particles for ALF treatment may be attributed to the unique properties of PAOX particles, which can target passively liver, stimulate cellular uptake and trigger a colloid osmotic disruption of the phagosome to release encapsulated PTX into the cytosol. Taken together, we believe that PAOX particles are a promising drug delivery candidate for the treatment of acute inflammatory diseases.     

Tetracycline adsorption on kaolinite: pH,metal cations and humic acid effects

Contamination of environmental matrixes by human and animal wastes containing antibiotics is a growing health concern. Because tetracycline is one of the most widely-used antibiotics in the world, it is important to understand the factors that influence its mobility in soils. This study investigated the effects of pH, background electrolyte cations (Li⁺, Na⁺, K⁺, Ca²⁺ and Mg²⁺), heavy metal Cu²⁺ and humic acid (HA) on tetracycline adsorption onto kaolinite. Results showed that tetracycline was greatly adsorbed by kaolinite over pH 3–6, then decreased with the increase of pH, indicating that tetracycline adsorption mainly through ion exchange of cations species and complexation of zwitterions species. In the presence of five types of cations (Li⁺, Na⁺, K⁺, Ca²⁺ and Mg²⁺), tetracycline adsorption decreased in accordance with the increasing of atomic radius and valence of metal cations, which suggested that outer-sphere complexes formed between tetracycline and kaolinite, and the existence of competitor ions lead to the decreasing adsorption. The presence of Cu²⁺ greatly enhanced the adsorption probably by acting as a bridge ion between tetracycline species and the edge sites of kaolinite. HA also showed a major effect on the adsorption: at pH < 6, the presence of HA increased the adsorption, while the addition of HA showed little effect on tetracycline adsorption at higher pH. The soil environmental conditions, like pH, metal cations and soil organic matter, strongly influence the adsorption behavior of tetracycline onto kaolinite and need to be considered when assessing the environmental toxicity of tetracycline.     

PAF和vWF在银杏叶片治疗不稳定型心绞痛中的作用

目的观察银杏叶片治疗不稳定型心绞痛（UAP）患者血小板活化因子（PAF）、血管性血友病因子（vWF）的变化,探讨其对心血管的保护作用及机制。方法检测26例健康对照者和61例UAP患者血浆PAF、vWF和PAR水平,将患者随机分为常规治疗组（对照组）和合并银杏叶片治疗组（银杏叶组）,分别于治疗前和治疗28d后测定PAF、vWF和PAR水平。结果UAP组PAF、vWF和PAR水平均显著高于正常对照组（P〈0．01）。治疗前,银杏叶组与对照组PAF、vWF和PAR水平无显著差异（P〉0．05）,银杏叶组治疗后PAF、vWF和PAR分别显著低于治疗前（P〈0．01）;对照组治疗前后PAF、vWF和PAR水平差异均无统计学意义（P〉0．05）。结论银杏叶片可通过下调PAF和vWF水平,降低血小板聚集程度从而可有效治疗不稳定型心绞痛。     

High expression of epidermal growth factor-like domain 7 is correlated with poor differentiation and poor prognosis in patients with epithelial ovarian cancer

Objective

The purpose of this study was to evaluate the expression of epidermal growth factor-like domain 7 (EGFL7) in epithelial ovarian cancer, and to assess its relevance to clinicopathological characteristics and patients'' survival.

Methods

A total of 177 patients with epithelial ovarian cancer were enrolled in the current study. For each patient, a retrospective review of medical records was conducted. Immunohistochemical staining for EGFL7 was performed using tissue microarrays made with paraffin-embedded tissue block. EGFL7 expression levels were graded on a grade of 0 to 3 based on the percentage of positive cancer cells. We analyzed the correlations between the expression of EGFL7 and various clinical parameters, and also analyzed the survival outcome according to the EGFL7 expression.

Results

The expression of EGFL7 in ovarian cancer tissues was observed in 98 patients (55.4%). High expression of EGFL7 (grade 2 or 3) was significantly correlated with pathologic type, differentiation, stage, residual tumor after debulking surgery, lymphovascular space involvement, lymph node metastasis, high cancer antigen 125, peritoneal cytology, and ascites. Among these clinicopathologic factors, differentiation was significantly correlated with EGFL7 expression in multivariate analysis (p<0.05). Survival analysis showed that the patients with high EGFL7 expression had a poorer disease free survival than those with low EGFL7 expression (p=0.002).

Conclusion

Our data suggest that EGFL7 expression is a novel predictive factor for the clinical progression of epithelial ovarian cancer, and may constitute a therapeutic target for antiangiogenesis therapy in patients with epithelial ovarian cancer.     

岩藻多糖联合标准四联疗法根除幽门螺杆菌的临床疗效观察

目的 观察岩藻多糖联合标准四联疗法根除幽门螺杆菌(HP)的疗效.方法 选取2019年6月-2020年1月于青岛大学附属医院就诊HP感染患者90例,随机分为标准四联疗法组(Ⅰ组)30例,标准四联疗法+岩藻多糖组(Ⅱ组)30例,岩藻多糖+标准四联序贯组(Ⅲ组)30例.Ⅰ组给予标准剂量的兰索拉唑、阿莫西林、克拉霉素、枸橼酸铋钾(标准四联)治疗2周;Ⅱ组给予岩藻多糖联合标准四联治疗2周以后,再仅给予岩藻多糖继续治疗4周;Ⅲ组先给予岩藻多糖治疗6周,复查13 C-尿素呼气试验(13 C-UBT),阳性者再给予标准四联治疗2周.在Ⅰ组治疗结束后4周、Ⅱ组治疗结束后立即、Ⅲ组标准四联治疗结束后4周时复查13 C-UBT,分析比较3组患者治疗前后HP根除、不良反应发生情况以及临床疗效.结果 治疗后3组患者的HP阴性比例差异无显著性(P>0.05);Ⅱ组、Ⅲ组患者治疗前后症状积分差值显著高于Ⅰ组(F=3.84,P<0.05);Ⅱ、Ⅲ组临床疗效优于Ⅰ组(Hc=13.39,q=4.29、5.63,P<0.05).结论 岩藻多糖联合标准四联疗法可有效根除HP,在进行标准四联治疗前或治疗同时添加岩藻多糖均可显著改善HP感染者临床症状,为充分开发岩藻多糖的潜在治疗价值提供了数据支持.