Stimulus-dependent activation of c-Fos in neurons and glia in the rat cerebellum

The intent of the present study was to use chemical or electrical stimulation of cerebellar afferents to determine how different stimulation paradigms affect the pattern of activation of different populations of neurons in the cerebellar cortex. Specifically, we analyzed immediate changes in neuronal activity, identified neurons affected by different stimulation paradigms, and determined the time course over which neuronal activity is altered. In the present study, we used either systemic (harmaline) or electrical stimulation of the inferior cerebellar peduncle (10 and 40 Hz) to alter the firing rate of climbing and mossy fiber afferents to the rat cerebellum and an antibody made against the proto-oncogene, c-fos, as a marker to identify activated neurons and glia. In control animals, only a few scattered granule cells express nuclear Fos-like immunoreactivity. Although no other cells show Fos-like immunoreactivity in their nuclei, Purkinje cells express Fos-like immunoreactivity within their somatic and dendritic cytoplasm in control animals. Within 15 min of chemical or electrical stimulation, numerous granule and glial cells express Fos-like immunoreactivity in their nuclei. Cells in the molecular layer express Fos-like immunoreactivity following harmaline stimulation in a time and lobule specific manner; they do not appear to be activated in the electrical stimulation paradigm. Following harmaline injections, there is an initial loss of Fos-like immunoreactivity in the cytoplasm of Purkinje cells; 90 min later, nuclear staining is observed in a few scattered Purkinje cells. Following electrical stimulation, the cytoplasmic staining in Purkinje cells is enhanced; it is never present in the nucleus. Data derived from this study reveal cell-specific temporal and spatial patterns of c-Fos activation that is unique to each paradigm. Further, it reveals the presence of an activity dependent protein in the cytoplasm of Purkinje cell somata and dendrites.     

抗SARS-CoV抗原的人源Fab段噬菌体抗体库的构建

目的 :利用抗SARS冠状病毒IgG抗体阳性的SARS康复患者外周血淋巴细胞 ,构建人源Fab段抗体文库。方法 :制备外周血淋巴细胞总RNA ,逆转录成cDNA。以其为模板 ,利用针对家族特异性Ig基因的引物扩增重链Fd段和轻链基因 ,并重组到噬菌粒载体pComb3中 ,将重组噬菌粒载体电转化大肠杆菌XL 1Blue,酶切鉴定抗体库的重组率 ,并测定噬菌体抗体库的库容量。结果 :构建了源于SARS康复患者血清中抗Fab段的抗体文库 ,轻链、重链Fd段基因的重组率分别为91%和 75 % ,库容量为 7.2 3× 10 7。结论 :成功地构建了抗SARS CoV抗原的人源Fab段噬菌体抗体库     

VALUE AND LIMITATIONS OF SINUS NODE FUNCTION TE.ST BY ATRIAL PACING IN SICK SINUS SYNDROME DIAGNOSIS

In order to evaluate the significance of the sinus node function test by transvenous atrial pacing in the diagno,sis of sick sinus syndrome (SSS), sinus node recovery time (SRT), co]rrected sinus node recovery time (CSRT) and total atrio- sinus and sino-atrial conduction time (SACT) are observed in 69 patients including non-SSS group 39 cases and SSS group 30 cases. According to our data, we suggest criteria for sinus node dysfunction as follows. SRT>1,400 ms and/or A-V junctional esca.pe before sinus recovery and;or secondary pause, CSRT>560 ms. total SACT>300 ms. With l item abnormal, the false positive rat.e in the non-SSS group is 7.7c7。 and the false negative rate in the S.SS group 3.30i" with 2 items abnormal, the false positive rate in the non-SSS group Oi and the false negative rate in the SSS grOiup 6.7'70. Atropine test results are compared with that of atrial pacing, 95.8To and 84.2% are compatible with each other in these 2 groups. In order to cut the use of invasive technic to the minimum, we propose performing the atropine test first. Only in those with unexplainable test results should the trial pacing test. be resorted to..     

皮下通道型胆囊肝胆管成形术治疗肝胆管结石 和胆管狭窄的临床研究

目的：总结皮下通道型胆囊肝胆管成形术的临床效果。方法：统计了1994年以来我院76例行STHG手术患者的适应症、临床效果及术后早期并发症。并选择同期实施的125 列行传统胆肠吻合（CJ）的患者进行对照。结果：STHG的手术适应症与传统胆肠吻合基本相同;STHG组患者的手术时间、住院时间、术中出血量明显少于CJ组,术后恢复良好,仅2例次并发症。结论;该术式既保存了胆囊及Oddi括约肌功能,又保证了胆汁的生理流向;防止了肠液的反,所以避免了消化功能紊乱,防止了反 流性胆管炎,对患者的影响小,是一种较为理想的治疗肝胆管结石和肝门胆管狭窄的术式。     

自发性高血压大鼠应用卡托普利加左旋精氨酸、牛磺酸的实验研究

目的：为研究左旋精氨酸、牛磺酸、卡托普利三药合用对自发性高血压大鼠的血压、血脂以及同同型半胱氨酸的影响。方法：用酶法测血脂,用高效液相色谱法测血浆HCY,在大鼠清醒状态下,测鼠尾的尾动脉收缩压,观察两组自发性高血压大鼠（卡托普利单用组,左旋精氨酸＋牛磺酸＋卡托普利三药合用组）在用药前后各指标的变化。结果：①经过四周药物治疗,合用组血压下隆比卡托普利组明显（P＜0.01）。②卡托普利组血脂无降低（P＞0.05）。合用组TC和TG均降低,（P＜0.005)。③两组HCY均下降。用药前,大鼠血压高低与血浆HCY的浓度无相关性（P＞0.05）,用药后,血压的下降与血浆HCY浓度的变化无相关性（P＞0.05）。结论：①卡托普利与有氨酸、牛磺酸合用对血压、血脂的控制比单用卡托普利。②血压的下降与同型半胱氨酸的下降无相关性（P＞0.05）。     

Relationship between the Mutation of IRS-1 Gene and β_3-adrenergic Receptor Gene

Ｏｂｅｓｉｔｙｉｓａｓｓｏｃｉａｔｅｄｗｉｔｈｉｎｓｕｌｉｎｒｅｓｉｓ ｔａｎｃｅ (ＩＲ) ,ｗｈｉｃｈｐｌａｙｓａｃｒｉｔｉｃａｌｒｏｌｅｉｎｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆｔｙｐｅ 2ｄｉａｂｅｔｅｓｍｅｌｌｉｔｕｓ.ＴｈｅｍｅｃｈａｎｉｓｍｏｆＩＲｉｓｒｅｌａｔｅｄｔｏｔｈｅａｂｎｏｒｍａｌｉｔｉｅｓｏｆｉｎｓｕｌｉｎｒｅｃｅｐｔｏｒ,ｐｒｅ ｒｅｃｅｐｔｏｒ,ｐｏｓｔ ｒｅｃｅｐ ｔｏｒ ,ｒｅｓｕｌｔｉｎｇｉｎｄｅｆｅｃｔｉｖｅｍｅｔａｂｏｌｉｃｐａｔｈｗａｙｏｆｇｌｕｃｏｓｅ,ｌｉｐｉｄａｎｄ ｐｒ…     

大鼠去势后胸腺变化的组织学及超微结构的观察

目的：探讨去势对大鼠胸腺淋巴细胞超微结构与细胞功能的影响。方法：分别于去势大鼠术后2周、4周、6周、8周后,取胸腺组织进行HE染色和透射电镜观察。结果：实验表明去势导致胸腺淋巴细胞结构坏死,或者细胞器严重受损。结论：去势后胸腺淋巴细胞形态和功能均受到损害。     

体外观察维甲酸对NIH/3T3细胞的抑制作用

目的评价维甲酸(RA)对NIH/3T     

银杏黄酮甙和甲基B_(12)联合治疗糖尿病周围神经病变的疗效观察

目的　探讨银杏黄酮甙和甲基B12 对糖尿病周围病变的治疗效果。方法　将 14 9例糖尿病周围神经病变患者随机分为 4组 ,分别为常规治疗组 (对照组 )、银杏黄酮甙组 (第 1组 )、甲基B12 组 (第 2组 )、银杏黄酮甙与甲基B12 联合组(第 3组 )。疗程为 8周。观察治疗前后自觉症状、腱反射和神经传导速度。结果　第 3组总有效率为 95 % ,显著高于对照组 (17% ) (P <0 .0 0 5 )。第 1组和第 2组 ,总有效率分别为 6 9%和 72 % ,与对照组比较均有显著差异 (P <0 .0 1)。第 3组神经传导速度治疗后较冶疗前显著改善 (P <0 .0 1) ,而且显著优于对照组 (P <0 .0 1)、第 1组 (P <0 .0 5 )和第 2组 (P <0 .0 5 )。结论　银杏黄酮甙和甲基B12 联合治疗糖尿病周围神经病变较单独应用更为有效。