全肝容积灌注CT成像评估兔肝血流灌注及体积对肿瘤坏死率的影响

目的 探讨全肝容积灌注CT成像（VPCT）评估兔肝血流灌注及体积对肿瘤坏死率的影响。方法 采用兔肝VX2移植瘤作为实验组,并设立对照组。实验组及假手术对照组在术后14 d通过全肝VPCT并获得相应的灌注参数,测量并计算实验组肿瘤体积（TV）、肿瘤坏死率（TNR）。检查完后对所有实验组肿瘤标本进行病理学观察。结果 实验组癌组织与对照组肝组织灌注血流量（BF）、灌注血容量（BV）、动脉灌注量（ALP）、门静脉灌注量（PVP）、动脉灌注指数（HPI）比较,差异均有统计学意义（P <0.05）,实验组癌旁组织与对照组肝组织BF、BV、ALP、PVP、HPI比较,差异均无统计学意义（P >0.05）。Pearson相关性分析显示,TNR与ALP、HPI呈负相关（r =-0.410和-0.356,均P <0.05）,TNR与BF、BV、PVP无相关性（r =-0.016、-0.155和0.256,均P >0.05）;TV与BF、BV、PVP、ALP、HPI无相关性（r =0.062、0.292、0.140、0.142和0.014,均P >0.05）;肝VX2移植瘤TNR与TV无相关性（r =-0.098,P >0.05）。HE染色发现肿瘤边缘区为富含血管的环形癌组织,中央为坏死区。结论 兔肝VX2移植瘤TNR与全肝VPCT反映的肿瘤动脉灌注高低有关,与TV无明显关联。     

A Study of Epileptiform Hippocampal Unit Activity Induced by Intracerebroventricular Injection of Kainic Acid in Rats

Hippocampal EEG and unit activities were recorded after seizures were initiated withintracerebroventricular injection of kainic acid (KA) in rats. Three kinds of hippocampal unit re-sponses to KA were found:1. Positive units: These units were characterized by high frequency burst firing temporally coincidentwith each hippocampal EEG spike.2. Negative units: These units showed a cessation of firing during each EEG paroxysm.3. Indifferent units: These units showed no evident chanses coincident with EEG paroxysms.Most positive units were hippocampal complex spike cells which correspond histologically tohippocampal pyramidal cells, and most complex spike cells fired in positive bursts after KA treat-ment. In the early period after KA injection, the positive units were concentrated in CA3 area. It was suggested that the activities of positive units may be considered as the typical epileptiformhippocampal unit activity induced by KA, and the firing features of negative units ma be the resultof the influence of hippocampal inhibitory interneurons or the result of excessive cellulardepolarization, and that hippocampal pyramidal cells were more sensitive to the epileptogenic ef-fect of KA than hippocampal intemeurons, and some pyramidal cells in CA3, in particular,may serve as "epileptic pacemaker neurons " in KA-induced epileptogenesis.     

散发性戊型肝炎病毒感染的诊断

用基因工程重组的戊型肝炎病毒基因结构区第二码框架和第二读码框架具有免疫表位的嵌合抗原，建立了间接酶联免疫法，检测散发性急性肝炎病人血清中抗－ＨＥＶＩｇＧ和ＩｇＭ抗体。在４６例急性肝炎病人中出抗－ＨＥＶＩｇＧ抗体阳性７例，阳性率为１５．２２％，７例ＩｇＧ抗体阳性中，有５例ＩｇＭ抗体也阳性，占７１．４％。     

Isolation and characterization of Mycoplasma sphenisci sp. nov. from the choana of an aquarium-reared jackass penguin (Spheniscus demersus)

Strain UCMJ was isolated from the choana of a jackass penguin (Spheniscus demersus) with recurrent mucocaseous choanal discharge. Isolation of this mycoplasma expands the known range of species hosting mycoplasmas. The name Mycoplasma sphenisci sp. nov. is proposed for this new species, for which strain UCMJ is the type strain.     

视神经管区的临床应用解剖研究

对５０个成人尸头和３０个干性颅骨标本的视神经管区进行了显微解剖学研究，测量了视神经管的长度、口径及管壁的厚度、观察了视神经管内壁的毗邻结构关系，还测量了一些与视神经管减压术有关的数据。讨论了经鼻外筛、蝶窦视神经管减压时值得注意的若干问题。     

慢性肾衰大鼠单个核细胞内游离钙浓度和白介素－1活性的变化

本文探讨了５／６肾切除术后慢性肾衰大鼠残肾纤维化的发生机理，发现术后１２０天，在残肾单个核炎性细胞浸润、残肾显著纤维化和肾功能损害的同时，残肾脂质过氧化物含量显著升高，抗氧化机制功能显著下降，钠钾ＡＴＰ酶活力显著下降，周围血单个核细胞内游离钙浓度显著升高，单个核细胞培养上清白介素－１活性增高。而摄入大量维生素Ｅ的大鼠，上述各指标有不同程度的改善，残肾纤维化显著减轻。提示残肾纤维化可能与单个核白细胞内游离钙浓度升高，进而产生白介素－１增多有关。     

Restriction endonuclease fingerprinting enhanced conformation sensitive gel electrophoresis (REF-CSGE) in the analysis of BRCA1 exon 11 mutations in a high-risk breast cancer cohort

Efficient genetic analysis of large exonic regions containing heterozygous mutations and common polymorphisms can be difficult. We have analyzed 30 patients for inherited susceptibility mutations (ISM) within exon 11 of the BRCA1 gene as part of an ongoing genetic epidemiological study of high-risk breast cancer (HRBC). A novel combination of restriction endonuclease fingerprinting (REF) and conformation sensitive gel electrophoresis (CSGE) was developed for rapid and efficient screening of mutations. This method (REF-CSGE) was compared side-by-side with standard CSGE and evaluated for both efficiency and sensitivity of detection. REF-CSGE detected 100% of the alterations found by CSGE. However, one variant was only detectable by REF-CSGE. All samples with variant bands were sequenced to confirm the nature of the alteration. In total, two small deletions (frameshifts) and 62 point mutations (60 known polymorphisms and two variants of unknown significance) were found in our cohort. The majority of the exon 11 polymorphisms detected are inherited as a linked haplotype. Point mutations that comprise these haplotypes could be simultaneously detected on a single gel by REF-CSGE, thereby decreasing the number of sequencing reactions necessary to elucidate heteroduplex patterns seen on CSGE gels. An analysis of the overall efficiency of both techniques revealed that REF-CSGE required 67% fewer confirmatory sequencing reactions, resulting in savings in both reagents and technician time.     

镉对培养大鼠睾丸支持细胞的损伤及黄芪的拮抗作用

目的:探讨镉对培养大鼠睾丸支持细胞的损伤及黄芪的保护作用.方法:对照组和经镉、镉加黄苠处理的原代培养大鼠睾丸支持细胞用于波形蛋白、E-钙黏蛋白免疫组织化学检测、细胞内钙离子浓度测定及电镜观察.结果:镉处理后2种蛋白阳性产物表达较对照组减弱.镉加黄芪组阳性产物较对照组减弱但明显强于镉组.对照组、镉和镉加黄苠组细胞内钙离子浓度分别为(81.773±3.711)、(161.6024±1.978)和(104.278±1.963).镉处理后支持细胞超微结构受损明显,部分细胞核异染色质凝聚或核碎裂,胞质空泡化.镉加黄芪组细胞破坏较轻.结论:镉对培养支持细胞具有明显的毒性作用,而黄苠可以拮抗镉对支持细胞的损伤.     

虚拟咬合运动初步仿真模拟的研究与实现

我们试验了利用VR技术进行虚拟咬合仿真制作的全部过程。首先,采用光学三维测量仪对上下颌石膏模型进行数字化,通过预处理获取有效的三角网格曲面模型;其次,对咬合运动模型进行合理的简化,分解为一系列的平移运动和旋转运动;通过动态刷新完成开闭口运动、侧移运动的计算机运动仿真,可视化地观察咬合运动;然后利用模型碰撞检测算法动态地计算咬合接触位,并详细地分析了咬合接触时的咬合点位置分布和咬合剖切面上的咬合点接触关系;最后讨论了目前虚拟咬合仿真存在的问题和今后研究的方向。     

散发性急性戊型肝炎血清抗体动态变化和肝脏超微…

为探讨散发性戊型肝为血清抗体动态变化，应用酶联免疫法（ＥＩＡ）检测了７例急性戊型肝炎抗戊型肝炎病毒（ＨＥＶ）ＩｇＧ和ＩｇＭ抗体、并对１例２进行了肝超微结构病理检测，结果表明，发病１０天至４５天内抗ＨＥＶ－ＩｇＧ和ＩｇＭ滴度最高，发病第４０天仍有肝细胞肿胀，胞浆空化和线粒体固缩等病理变化。患者轿清抗－ＨＥＶＩｇＭ滴度在４５天后逐渐下降，２个月内全中消失，抗－ＨＥＶＩｇＭ滴度在４５天后逐渐下降，２个月