全文获取类型
收费全文 | 5425篇 |
免费 | 560篇 |
国内免费 | 515篇 |
专业分类
耳鼻咽喉 | 36篇 |
儿科学 | 99篇 |
妇产科学 | 38篇 |
基础医学 | 603篇 |
口腔科学 | 71篇 |
临床医学 | 688篇 |
内科学 | 893篇 |
皮肤病学 | 74篇 |
神经病学 | 357篇 |
特种医学 | 252篇 |
外国民族医学 | 10篇 |
外科学 | 514篇 |
综合类 | 1092篇 |
现状与发展 | 2篇 |
预防医学 | 320篇 |
眼科学 | 136篇 |
药学 | 617篇 |
2篇 | |
中国医学 | 315篇 |
肿瘤学 | 381篇 |
出版年
2024年 | 19篇 |
2023年 | 84篇 |
2022年 | 208篇 |
2021年 | 306篇 |
2020年 | 201篇 |
2019年 | 193篇 |
2018年 | 199篇 |
2017年 | 171篇 |
2016年 | 164篇 |
2015年 | 253篇 |
2014年 | 324篇 |
2013年 | 237篇 |
2012年 | 433篇 |
2011年 | 414篇 |
2010年 | 266篇 |
2009年 | 200篇 |
2008年 | 275篇 |
2007年 | 265篇 |
2006年 | 296篇 |
2005年 | 265篇 |
2004年 | 217篇 |
2003年 | 235篇 |
2002年 | 195篇 |
2001年 | 169篇 |
2000年 | 148篇 |
1999年 | 140篇 |
1998年 | 79篇 |
1997年 | 80篇 |
1996年 | 78篇 |
1995年 | 63篇 |
1994年 | 49篇 |
1993年 | 38篇 |
1992年 | 48篇 |
1991年 | 46篇 |
1990年 | 32篇 |
1989年 | 25篇 |
1988年 | 26篇 |
1987年 | 18篇 |
1986年 | 17篇 |
1985年 | 15篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 1篇 |
1981年 | 3篇 |
排序方式: 共有6500条查询结果,搜索用时 281 毫秒
81.
穴位注射加灸治疗小儿腹泻疗效观察 总被引:2,自引:1,他引:2
目的 观察穴位注射加灸治疗小儿腹泻的疗效。方法 选用庆大霉素和 6 5 4 - 2肌注、穴注、穴注加灸治疗小儿腹泻 ,每日 1次 ,3次后比较 3组疗效。结果与结论 穴注组治愈率明显高于肌注组 (P<0 .0 5 ) ,穴注加灸组治愈率明显高于穴注组(P<0 .0 0 5 ) 相似文献
82.
Anticancer Drug Resistance of HeLa Cells Transfected With Rat Glutathione S-transferase pi Gene 总被引:3,自引:0,他引:3
Cao W Zuo J Meng Y Wei Q Shi ZH Ju LM Fang FD 《Biomedical and environmental sciences : BES》2003,16(2):157-162
Objective To establish a cytologic expressing system of rat glutathione S-transferase pi (GST-pi) cDNA for detecting the resistance of HeLa cells to anticancer drugs. Methods The assessment was made with various anticancer drugs (adriamycin, mitomycin, cisplatinum and vincristine) that showed different cytotoxicities in transfectant HeLa cells with pSV-GT containing rat GST-pi cDNA (HeLa/pSV-GT) or control pSV-neo (HeLa/pSV-neo). Expression levels of GST-pi mRNA in HeLa/pSV-GT and HeLa/pSV-neo were measured by in situ hybridization using Digoxin-labelled cDNA probe. Results HeLa/pSV-GT expressed significantly high degree of GST-pi mRNA, whereas both HeLa/pSV-neo and HeLa cells had very low expression. Cytotoxicities of HeLa/pSV-GT and HeLa/pSV-neo with 4 anticancer drugs were measured by MTT assay. Drug concentrations for yielding 50% inhibition (IC50) in HeLa/pSV-GT by adriamycin, mitomycin and cisplatinum were 70.13μg/mL, 10.95μg/mL and 16.52μg/mE respectively. In contrast, IC50 in HeLa/pSV-neo was 10.34μg/mL, 7.48μg/mL and 13.70μg/mE respectively. The cytotoxicities of vincristine on both HeLa/pSV-GT and HeLa/pSV-neo were not significantly different. Conclusions Our findings suggest that HeLa/pSV-GT containing rat GST-pi cDNA is resistant to some anticancer drugs due to overexpression of GST-pi. Also, HeLa/pSV-GT cell line could serve as a useful cytogenetic model for further research. 相似文献
83.
84.
85.
目的 探讨合并伤对颅脑损伤病死率的影响及其急救原则。方法 总结分析我院收治的 4 311例颅脑损伤病例合并伤资料。结果 颅脑损伤 4 311例中合并伤 1372例 ( 31 8% ) ,其中轻型组 13 7% ,中型组 2 7 9% ,重型及特重型组 5 3 9%。各部位合并伤在总体、<2 4h死亡 ( 16 4例 )组、>2 4h死亡 ( 2 2 9例 )组中的发生率依次为 :颌面部 12 2 %、14 6 %、12 6 % ;胸部 9 3%、32 9%、10 9% ;腹部 1 5 %、9 1%、3 4 % ;脊柱骨盆 3%、2 1 9%、4 8% ;四肢 17 3%、2 0 1%、19 2 %。结论 在颅脑损伤患者的院前、院内急救中要加强对胸部、腹部、脊柱骨盆合并伤的合理救治 相似文献
86.
Objective: To observe the behavioral and biochemical effects of a traditional Chinese medicine Huanshao Dan (HSD) on learning and memory
deficits in transient cerebral ischemia model in mice.Methods: Step-through experiments, receptor binding test and choline acetyltransferase (ChAT) activities determination were performed.Results: Mice undertaken transient ischemia commited much more mistakes in step-through experiments and showed relatively higher3 H-MK801 binding in cerebral cortex and hippocampus than in sham operated animals. HSD decoction was most effective in reducing
these mistakes in mice. At the same time, and3 H-MK801 binding of cerebral cortex and hippocampus tissues were also significantly decreased, while ChAT activities in the
same tissues were increased.Conclusion: HSD might antagonize ischemic injury of brain through inhibition of glutamate N-methyl-D-Aspartic acid receptor overactivity.
ΔThis program was supported by National Nature Science Foundation of China (No.39421012) 相似文献
87.
Karl T. Kelsey Margaret R. Spitz Zheng-Fa Zuo John K. Wiencke 《Cancer causes & control : CCC》1997,8(4):554-559
The genes coding for separate isoforms of both the human glutathioneS-transferase class mu and class theta enzymes (GSTM1and GSTT1) arepolymorphic with a variable ethnic distribution. These enzymes detoxifyreactive epoxides, including carcinogens produced by tobacco smoke. Becauseof this, the null polymorphism in the GSTM1 gene (coding for the glutathioneS-transferase class mu enzyme) has been studied widely as a possible sourceof inherited susceptibility to smoking-related lung cancer. The more recentlydescribed null polymorphism in the GSTT1 gene also could contribute to anincreased risk of smoking-related lung cancer. As the incidence of lungcancer is known to differ by ethnicity, we have conducted a case-controlstudy in the United States of 108 African-Americans (Blacks) and 60Mexican-Americans (Hispanics) with lung cancer and 132 African-American(Black) and 146 Mexican-American (Hispanic) controls to investigate theassociation of the GSTT1 and GSTM1 polymorphi sms with lung cancer inminority populations. In the unadjusted data, there was a borderlinesignificant association of the GSTM1 null polymorphism with lung cancer inMexican-Americans (odds ratio [OR] = 1.8, 95 percent confidence interval [CI]= 1.0-3.3 ) that was not observed in African-Americans. The GSTT1 nullpolymorphism also had a higher prevalence in cases than controls in bothracial/ethnic groups, but this increase was not statistically significant.When the data were analyzed using logistic regression controlling for age,gender, race, and smoking, no significant association of either trait withlung cancer was observed, with ORs for both traits of approximately 1.3.However, when the prevalence of individuals who were null for bothpolymorphisms was compared by case status, a significant interaction wasobserved. Logistic regression models showed the OR for the association oflung cancer and the presence of both null polymorphisms compared with one(either GSTT1 or GSTM1) or no null genotype to be 2.9 (P < 0.04). Theseresults suggest that there may be carcinogenic intermediates in cigarettesmoke that are substrates for both the GSTT1 and GSTM1 enzymes, and that lungcancer risk is increased more than additively for individuals who have bothGSTT1 and GSTM1 null polymorphisms. 相似文献
88.
目的 :探讨抗疱疹病毒药物黄芪A6 组分(A6)和无环鸟苷(ACV)联合抗 1型单纯疱疹病毒(HSV1 )的作用机制。方法 :利用竞争PCR检测A6 和ACV联合抗HSV1 的协同作用 ,并与细胞病变(CPE)抑制法进行比较。结果 :竞争PCR测定A6、ACV和A6 ACV对HSV1 的最小抑制浓度(MIC)分别为 1 88mg/ml、3 3 7μg/ml和 0 47mg/ml 0 84μg/ml;CPE抑制法测定A6、ACV和A6 ACV对HSV1 的MIC分别为 6 2 5mg/ml、5 0 μg/ml和 0 94mg/ml 1 2 5 μg/ml;联合抑制指数的分数(FICI)均小于 0 5 ;显示明显的联合协同抑制作用。结论 :定量PCR是筛选抗病毒药物的有效方法 ;结果还表明A6 和ACV对HSV1 的抑制作用主要表现在病毒增殖周期的复制阶段。 相似文献
89.
TheZiWuLiuZhu(I4gu)theoryofacupunctureandmoxibustionholdsthathumanqiandbloodcirculate,flowandebbinthetwelvechannelsfollowingacertainlawinthetraditionaltwelvetwo-hourperiodsofaday.Thisperiodical,directional,sequentialcirculationmakesfunctiona1activityofvis… 相似文献
90.