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101.
Jairo L. Rodrigues Juliana M. Serpeloni Bruno L. Batista Samuel S. Souza Fernando Barbosa Jr 《Archives of toxicology》2010,84(11):891-896
Methylmercury (Met-Hg) is one the most toxic forms of Hg, with a considerable range of harmful effects on humans. Sodium ethyl
mercury thiosalicylate, thimerosal (TM) is an ethylmercury (Et-Hg)-containing preservative that has been used in manufacturing
vaccines in many countries. Whereas the behavior of Met-Hg in humans is relatively well known, that of ethylmercury (Et-Hg)
is poorly understood. The present study describes the distribution of mercury as (-methyl, -ethyl and inorganic mercury) in
rat tissues (brain, heart, kidney and liver) and blood following administration of TM or Met-Hg. Animals received one dose/day
of Met-Hg or TM by gavage (0.5 mg Hg/kg). Blood samples were collected after 6, 12, 24, 48, 96 and 120 h of exposure. After
5 days, the animals were killed, and their tissues were collected. Total blood mercury (THg) levels were determined by ICP-MS,
and methylmercury (Met-Hg), ethylmercury (Et-Hg) and inorganic mercury (Ino-Hg) levels were determined by speciation analysis
with LC-ICP-MS. Mercury remains longer in the blood of rats treated with Met-Hg compared to that of TM-exposed rats. Moreover,
after 48 h of the TM treatment, most of the Hg found in blood was inorganic. Of the total mercury found in the brain after
TM exposure, 63% was in the form of Ino-Hg, with 13.5% as Et-Hg and 23.7% as Met-Hg. In general, mercury in tissues and blood
following TM treatment was predominantly found as Ino-Hg, but a considerable amount of Et-Hg was also found in the liver and
brain. Taken together, our data demonstrated that the toxicokinetics of TM is completely different from that of Met-Hg. Thus,
Met-Hg is not an appropriate reference for assessing the risk from exposure to TM-derived Hg. It also adds new data for further
studies in the evaluation of TM toxicity. 相似文献
102.
Braulio Insuasty Alexander Gutiérrez Jairo Quiroga Rodrigo Abonia Manuel Nogueras Justo Cobo Laura Svetaz Marcela Raimondi Susana Zacchino 《Archiv der Pharmazie》2010,343(1):48-53
The present work describes the synthesis and antifungal evaluation of new 5‐arylidene‐(Z)‐2‐dimethylamino‐1,3‐thiazol‐4‐ones 4a – f , obtained by the reaction of aromatic aldehydes 1 and rhodanine 2 followed by treatment with DMF. All compounds were tested against a panel of yeasts, hialohyphomycetes, and dermatophytes using the microbroth dilution method. Compounds 4a and 4c showed antifungal activity, with compound 4a being the most active one. Compound 4a demonstrated to be fungicidal rather than fungistatic and selective activity against Cryptococcus neoformans and dermatophytes. MIC100, MIC80, and MIC50 of 4a were determined against a panel of clinical isolates of C. neoformans showing ranges of MICs between 2 and 16 μg/mL. 相似文献
103.
Jairo Jose Matozinho Cubas Ricardo Santos Sim?es Ricardo Martins Oliveira-Filho Manuel Jesus Sim?es Edmund C Baracat José Maria Soares Jr 《Clinics (S?o Paulo, Brazil)》2010,65(7):703-708
OBJECTIVE:
To analyze the amount of glycosaminoglycans in the uterine cervix during each phase of the rat estrous cycle.DESIGN:
Based on vaginal smears, forty female, regularly cycling rats were divided into four groups (n = 10 for each group): GI – proestrous, GII – estrous, GIII – metaestrous and GIV – diestrous. Animals were sacrificed at each phase of the cycle, and the cervix was immediately removed and submitted to biochemical extraction and determination of sulfated glycosaminoglycans and hyaluronic acid. The results were analyzed by ANOVA followed by the Bonferroni post-hoc test.RESULTS:
The uterine cervix had the highest amount of total sulfated glycosaminoglycans and dermatan sulfate during the estrous phase (8.90 ± 0.55 mg/g of cetonic extract, p<0.001; and 8.86 ± 0.57 mg/g of cetonic extract, p<0.001). In addition, there was more heparan sulfate at the cervix during the proestrous phase (0.185 ± 0.03 mg/g of cetonic extract) than during any other phase (p<0.001). There were no significant changes in the concentration of hyaluronic acid in the uterine cervix during the estrous cycle.CONCLUSION:
Our data suggest that the amount of total sulfated glycosaminoglycans may be influenced by hormonal fluctuations related to the estrous cycle, with dermatan sulfate and heparan sulfate being the glycosaminoglycans most sensitive to hormonal change. 相似文献104.
105.
ángela Domínguez Rosa Emilia Ballesteros Jairo Hernán Viáfara Oscar Mario Tamayo 《World Journal of Methodology》2013,3(2):19-26
AIM: To validate the protocol described here to be used in future clinical trials related to the effect of laser therapy on dental pulp.METHODS: Histologically treated samples from eight human healthy premolar teeth obtained from the middle root level were distributed in four groups: group 1 (G1) absolute control; group 2 (G2) only laser irradiation; group 3 (G3) exposed only to orthodontics; and group 4 (G4) treated with orthodontics and laser. Laser treatment was performed at 830 nm wavelength, 100 mW (energy 80 J/cm2, 2.2 J), for 22 s in the vestibular surface and 22 s in the palatal surface, 1 mm away from the dental root mucosa. Three staining methods were performed: hematoxylin-eosin (HE), Masson’s Trichrome method and Gomori’s method.RESULTS: The pulp histology parameters were evaluated and the results classified in to 3 parts: an inflammatory response, soft tissue response (dental pulp) and hard tissue response (dentin and predentin). There was no inflammation (chronic or acute) in any of the evaluated groups. The zones of pulp necrosis were found in one premolar of G3 and in one of G4; in groups G2 and G4 there was higher angiogenesis than in the other two groups. G4 group presented the highest level of vascularization. A reduced nerve density was observed in G3. A G2 specimen showed increased nerve density. A higher rate of calcification was observed in G1 compared to G2. Denticles, either real or false, were observed in G1, G2 and G3. Sclerosis of dentin and focal dentin loss was observed among all the groups. Secondary dentin was present in one sample in G1 and G2. A necrosis zone was found in one sample of G3 and G4. No differences between groups were observed in the odontoblast irregularity layer but the layer was wider in the group treated with laser only. A notable difference was detected in reduction of the cell-free layer between the groups G1 and G4. The findings in pulp tissue favor its adaptative response against dental movement induced by orthodontics. No definitive conclusions may be derived as this is a pilot study.CONCLUSION: The protocol described here was shown to be an effective method to evaluate changes in dental pulp submitted to low level laser in teeth under orthodontic movement. 相似文献
106.
Three-dimensional levitation culture improves in-vitro growth of secondary follicles in bovine model
Deize de Cássia Antonino Mayara Mafra Soares Jairo de Melo Júnior Paula Batista de Alvarenga Renata de Freitas Ferreira Mohallem Carina Diniz Rocha Luis Alberto Vieira Aline Gomes de Souza Marcelo Emílio Beletti Benner Geraldo Alves José Octavio Jacomini Luiz Ricardo Goulart Kele Amaral Alves 《Reproductive biomedicine online》2019,38(3):300-311
Research question
Does a three-dimensional culture system based on magnetic levitation with nanoparticles assembly maintain the follicular structure and viability with adequate growth rates leading to oocyte maturation after long-term culture?Design
Randomized-controlled trial of treatments in a bovine model. Secondary follicles (n?=?213) isolated from bovine ovaries were cultured in a two-dimensional system (two-dimensional control) or three-dimensional levitation system with different concentrations (three-dimensional 50 µl/ml, 100 µl/ml and 200 µl/ml) of magnetic nanoparticles. Follicular growth (diameter, daily growth and growth patterns), morphology (normal, degenerated and extruded follicles), antrum formation, oocyte viability and chromatin configuration were assessed.Results
Secondary follicles of three-dimensional 200-µl/ml treatment showed higher viability, antrum formation and lower degeneration rates than two-dimensional control. Also, follicles cultured in the three-dimensional 200-µl/ml treatment presented a most homogenous daily growth rate as shown by the lowest variance and standard deviation. Compared with the two-dimensional control, the proportion of non-growing and slow-growing follicles were 3.8-fold lower and 1.6-fold higher, respectively, in the three-dimensional 200-µl/ml treatment. After in-vitro maturation, the three-dimensional 200-µl/ml had a greater proportion of viable oocytes (1.7-fold) and meiotic resumption rates (2.4-fold) than the two-dimensional control treatment.Conclusion
The three-dimensional levitation culture system improves the viability of in-vitro development of bovine secondary follicles, antrum formation and lower extrusion and degeneration rates and adequate growth rate leading to relevant oocyte viability and meiotic resumption after in-vitro maturation. This approach does not require a specific medium, and has the potential as an alternative method to in-vitro follicle culture in several species, including humans. 相似文献107.
Rodrigues JL de Magalhães CS Luccas PO 《Journal of pharmaceutical and biomedical analysis》2005,36(5):1119-1123
A flow analysis (FA) system with spectrophotometric detection for Al determination in hemodialysis solutions was developed. The method was based on the reaction of Al with eriochrome cyanine R (ECR). The complex formed associated with cetyltrimethylammonium bromide (CTAB) — a cationic surfactant, which showed enough sensitivity to execute the direct analyte determination. All interferences were eliminated with the matrix matching calibration. The system presented the following analytic parameters: sensitivity (m) of 8.10 × 10−4 L μg−1, limit of detection (LOD) of 3.24 μg L−1 (3σ), linear correlation coefficient of 0.9966 and linear range response from 10.8 to 650 μg L−1. The accuracy of the proposed method was checked by comparison with electrothermal atomic absorption spectrometry (ET-AAS) method. There were no differences among the results obtained from both methods, at a confidence level of 95% (paired t-test). Recovery tests were also made, values obtained were from 90.4 to 109 of recovery for Al-spiked samples. 相似文献
108.
109.
Vasconcelos MM Malheiros AF Werner J Brito AR Barbosa JB Santos IS Lima DF 《Arquivos de neuro-psiquiatria》2005,63(1):68-74
OBJECTIVE: To analyze the contribution of psychosocial risk factors for the diagnosis of attention deficit/hyperactivity disorder (ADHD). METHOD: This is a retrospective, case-control study that analyzed 403 pupils from a public elementary school. DSM-IV symptom questionnaire and a specific psychosocial questionnaire were filled out, and a history and complete physical and neurological examinations were performed. RESULTS: The most statistically significant psychosocial risk factor was "marital discord in the past" (odds ratio = 11.66, p = 0.044). A total of 29 murders of subjects' close relatives were observed, and this variable was statistically relevant. CONCLUSION: Children that were exposed to marital discord in the past between parents underwent a 11.66-fold increase in the risk of ADHD diagnosis. Authors suggest that exposure to community violence be included among Rutters's indicators of adversity, which predispose infants and children to the future occurrence of ADHD and other neuropsychiatric disorders. 相似文献
110.