Surface analysis and corrosion resistance of different stainless steel orthodontic brackets in artificial saliva

The purpose of this study was to investigate the variation in corrosion resistance of commercial stainless steel (SS) brackets with different brands and types for the same application, using the electrochemical technique. The linear polarization test was used to evaluate the corrosion resistance, in terms of polarization resistance (Rp), of as-received commercial SS brackets in acidic artificial saliva. A two-way analysis of variance was used to analyze the Rp with the factors of brand and type. A scanning electron microscope and an atomic force microscope were used to analyze the surface morphology and roughness, respectively. The X-ray photoelectron spectroscopy was used to identify the chemical composition of the passive film on SS brackets. Results showed that different brands of SS brackets had a statistically significant difference in Rp (P < .0001), whereas there was no statistical difference between the bracket types ("Roth" and standard) (P = .27). Different surface topography, including surface roughness and defect, was present among the tested SS brackets. The same passive film structure, containing Cr2O3/Fe2O3 with small amounts of NiO, was observed on all SS brackets. The surface topography of the commercial SS brackets with identical surface passive film structure did not correspond with the difference in corrosion resistance.     

Technology Development to Explore the Relationship Between Oral Health and the Oral Microbial Community

The human oral cavity contains a complex microbial community that, until recently, has not been well characterized. Studies using molecular tools have begun to enumerate and quantify the species residing in various niches of the oral cavity; yet, virtually every study has revealed additional new species, and little is known about the structural dynamics of the oral microbial community or how it changes with disease. Current estimates of bacterial diversity in the oral cavity range up to 700 species, although in any single individual this number is much lower. Oral microbes are responsible for common chronic diseases and are suggested to be sentinels of systemic human diseases. Microarrays are now being used to study oral microbiota in a systematic and robust manner. Although this technology is still relatively young, improvements have been made in all aspects of the technology, including advances that provide better discrimination between perfect-match hybridizations from non-specific (and closely-related) hybridizations. This review addresses a core technology using gel-based microarrays and the initial integration of this technology into a single device needed for system-wide studies of complex microbial community structure and for the development of oral diagnostic devices.     

Sudden change of a large congenital melanocytic nevus to neurocutaneous melanosis

Neurocutaneous melanosis (NCM) is a congenital phakomatosis in which large congenital melanocytic nevi are associated with a benign or malignant melanocytic tumor of the leptomeninges. Because the prognosis of patients with symptomatic NCM is poor, it is essential to monitor the large congenital melanocytic patient regularly for a neurological evaluation. However, it has not been reported how quickly the lesion could appear. We observed a case of NCM suddenly developing in a large congenital melanocytic nevi patient. With this case, the NCM had developed within six months and was aggravated during the subsequent six months.     

Concurrence of replicative senescence and elevated expression of p16(INK4A) with subculture-induced but not calcium-induced differentiation in normal human oral keratinocytes

Primary normal human oral keratinocytes (NHOKs) undergo differentiation in the presence of calcium concentrations higher than 0.15 mM in vitro, which is useful in investigating the mechanisms involved in the differentiation of epithelial cells. Serial subculture of NHOKs to the postmitotic stage also induces terminal differentiation. However, the detailed mechanisms of both differentiation processes remain substantially unknown. To investigate the molecular differences in these processes, NHOKs were induced to differentiate by exposure to 1.2 mM of calcium and by serial subculture to the postmitotic stage. To study whether the cells were induced to differentiate and to undergo replicative senescence, the amount of cellular involucrin and the expression of senescence-associated beta-galactosidase (SA-beta-gal) were measured respectively. The expression of replicative senescence-associated genes and the activity of telomerase from the differentiated cells were also determined. Both calcium treatment and serial subculture to the postmitotic stage notably elevated the cellular involucrin. The percentage of SA-beta-gal-positive cells was significantly elevated by the continued subculture, but such changes were not observed in keratinocytes exposed to calcium. The concentration of cellular p16(INK4A) protein was progressively increased by the continued subculture but was not changed by calcium treatment. On the other hand, the concentrations of cellular p53 were similar in both differentiation processes. However, telomerase activity was lost in NHOKs that had undergone differentiation by both calcium treatment and serial subculture. The results indicate that calcium-induced differentiation of NHOKs has similar characteristics to their serial subculture-induced differentiation, but that the differentiation processes are not identical, because calcium-induced differentiation does not concur with either replicative senescence or the gradually increased concentration of p16(INK4A).     

Eugenol inhibits K+ currents in trigeminal ganglion neurons

Eugenol, a natural capsaicin congener, is widely used in dentistry. Eugenol inhibits voltage-activated Na(+) and Ca(2+) channels in a transient receptor potential vanilloid 1 (TRPV1)-independent manner. We hypothesized that eugenol also inhibits voltage-gated K(+) currents, and investigated this in rat trigeminal ganglion neurons and in a heterologous system using whole-cell patch clamping. Eugenol inhibited voltage-gated K(+) currents, and the inhibitory effects of eugenol were observed in both capsaicin-sensitive and capsaicin-insensitive neurons. Pre-treatment with capsazepine, a well-known antagonist of TRPV1, failed to block the inhibitory effects of eugenol on K(+) currents, suggesting no involvement of TRPV1. Eugenol inhibited human Kv1.5 currents stably expressed in Ltk(-) cells, where TRPV1 is not endogenously expressed. We conclude that eugenol inhibits voltage-gated K(+) currents in a TRPV1-independent manner. The inhibition of voltage-gated K(+) currents is likely to contribute to the irritable action of eugenol. Abbreviations: human Kv1.5 channel, hKv1.5; transient receptor potential vanilloid 1, TRPV1.     

Activation of vanilloid receptor 1 (VR1) by eugenol

The structural similarity of eugenol with capsaicin suggests that these two agents may share molecular mechanisms to produce their effects. We investigated the effects of eugenol in comparison with those of capsaicin using whole-cell patch clamp and Fura-2-based calcium-imaging techniques in a heterologous expression system and with sensory neurons. In vanilloid receptor 1 (VR1)-expressing human embryonic kidney (HEK) 293 cells and trigeminal ganglion (TG) neurons, eugenol activated inward currents, whereas capsazepine, a competitive VR antagonist, and ruthenium red (RR), a functional VR antagonist, completely blocked eugenol-induced inward currents. Moreover, eugenol caused elevation of [Ca(2+)](i), and this was completely abolished by both capsazepine and ruthenium red in VR1-expressing HEK 293 cells and TG neurons. Our results provide strong evidence that eugenol produces its effects, at least in part, via VR1 expressed by the sensory nerve endings in the teeth.     

Sonicated extract of Enterococcus faecalis induces irreversible cell cycle arrest in phytohemagglutinin-activated human lymphocytes

The purpose of this study was to examine whether the sonicated extract of Enterococcus faecalis (SEF) alters the cell cycle transition of lymphocytes and thus regulates the fate of the arrested cells. Human lymphocytes were activated by phytohemagglutinin in the presence or absence of SEF, and cell cycle was assessed by flow cytometry. Seventy-two hours after activation with phytohemagglutinin, cells were activated from G0/G1 to S (6.1%) and G2/M (3.8%) phases of the cell cycle. In contrast, pretreatment with SEF resulted in 90.5% of cells remaining in G0/G1, and cell cycle progression to the S and G2/M phases was consequently inhibited. Caspase assay demonstrated that SEF-treated cells exhibited significantly increased apoptosis (56.7%) compared with phytohemagglutinin alone (28.1%). We propose that if this irreversible cell cycle arrest induced by E. faecalis occurs in vivo, it may result in local immunosuppression and contribute to the pathogenesis of endodontic failure. Our findings that E. faecalis can inhibit lymphocyte responses may be of particular relevance to the pathogenesis of endodontic failure. Although the immunologic mechanism involved in the pathogenesis of persistent periapical lesion is not clearly defined, it is reasonable to predict that the altered immune reaction may be linked to the immunosuppressive potential of E. faecalis or other oral bacteria.     

Parents' self-reported compliance with preventive practices after witnessing their child undergo intravenous sedation for dental treatment

The purpose of this study was to evaluate the self-reported compliance of parents with preventive dental care measures after witnessing their child undergo full-mouth rehabilitation under i.v. sedation. Records of 251 pediatric patients who underwent full-mouth rehabilitation under i.v. sedation between 1995 and 1999 at the University Children's Dental Center in Los Angeles, California were evaluated. Demographic data and rates of post sedation follow-up preventive visits were collected. In addition, seventy-five parents participated in a telephone interview and answered five multiple-choice questions that focused on the following areas: nursing habits, diet, hygiene, and follow-up preventive visits pre and post sedation. Parental attitude towards the experience was also assessed. Only 47 percent of the patients returned for at least one post-op/recall visit at the Children's Dental Center. Seventy-six percent of parents interviewed stated they made improvements in their child's hygiene post sedation. Only 45 percent of parents stated they made improvements in their child's diet and only 47 percent stated that they increased their child's dental recall visits post sedation. Eighty-eight percent of parents stated that they would be willing to have their children undergo i.v. sedation again if necessary for dental treatment. Parental presence during a child's dental rehabilitation via i.v. sedation does not change the preventive behaviors of these parents post sedation when compared to results of other studies.