脊髓损伤后的婚姻结局

<正> 本研究的目的是评价脊髓损伤(SCI)后结婚又离婚的年离婚率,并与人口中年龄、性别相似的非SCI者中的离婚率相比较,探讨离婚增多的可能因素。 作者这项研究是利用美国全国脊髓损伤统计中心(NSCISC)的数据进行的。NSCISC数据收集中包括病人的人口统计学、紧急救护和康复经历以及治疗结果,也收集有病人状况的年度随访资料和受伤前的个人经历,其中包括对伤后婚姻状况的改变,例如由单身而结婚、离婚,或由寡妇、鳏夫而再婚等。在该基础上本研究包括伤后结婚共622例。对少数     

Osteogenic differentiation of human bone marrow stromal cells on partially demineralized bone scaffolds in vitro

Tissue engineering has been used to enhance the utility of biomaterials for clinical bone repair by the incorporation of an osteogenic cell source into a scaffold followed by the in vitro promotion of osteogenic differentiation before host implantation. In this study, three-dimensional, partially demineralized bone scaffolds were investigated for their ability to support osteogenic differentiation of human bone marrow stromal cells (BMSCs) in vitro. Dynamic cell seeding resulted in homogeneous cell attachment and infiltration within the matrix and produced significantly higher seeding efficiencies when compared with a conventional static seeding method. Dynamically seeded scaffolds were cultured for 7 and 14 days in the presence of dexamethasone and evaluated on biochemical, molecular, and morphological levels for osteogenic differentiation. Significant elevation in alkaline phosphatase activity was observed versus controls over the 14-day culture, with a transient peak indicative of early mineralization on day 7. On the basis of RT-PCR, dexamethasone-treated samples showed elevations in alkaline phosphatase and osteocalcin expression levels at 7 and 14 days over nontreated controls, while bone sialoprotein was produced only in the presence of dexamethasone at 14 days. Scanning electron microscopy evaluation of dexamethasone-treated samples at 14 days revealed primarily cuboidal cells indicative of mature osteoblasts, in contrast to nontreated controls displaying a majority of cells with a fibroblastic cell morphology. These results demonstrate that partially demineralized bone can be successfully used with human BMSCs to support osteogenic differentiation in vitro. This osseous biomaterial may offer new potential benefits as a tool for clinical bone replacement.