大型公立医院实行院务公开制度的实践探索

为了进一步提高公立医院的透明度,通过在社会、患者、内部职工等各个层面开展院务公开工作,提升了医院外部形象,使患者对公立医院的内部结构、就医流程、个人账目等一目了然,改善了医患之间的关系,提高了医生和患者的满意度,同时有助于杜绝腐败现象的发生。     

门诊血液标本采集患者检验咨询服务需求的质性研究

目的了解门诊血液标本采集患者采血检验的真实感受和体验,寻求门诊开展检验咨询服务的方向和方法。方法采用解释现象学质性研究方法对8名成人患者及5名患儿家长进行半结构式深度访谈,了解其对血液标本采集检验的态度、检验咨询服务需求等,运用类属分析法归纳主题。结果共提炼出5个主题,包括采血患者准备知识的需求、标本采集送检知识的需求、检验结果咨询服务的需求、检验结果领取服务的需求、检验项目价格。结论在患者血液标本采集过程中,应关注患者及家属的检验咨询服务需求,有针对性的开展多种形式的检验咨询服务,提高检验质量,满足患者健康教育需求。     

Visualizing the endocytosis of phenylephrine in living cells by quantum dot-based tracking

To study the intracellular receptor-drug transportation, a fluorescent probe consisting of phenylephrine–polyethylene glycol-quantum dots conjugate was employed to track endocytosis process of phenylephrine in living cells. This type of movement was studied by continuously filming fluorescent images in the same cell. We also calculated the movement parameters, and divided the endocytosis process into 6 stages. Furthermore, the movement parameters of this probe in different organelles were determined by co-localization of the probe fluorescent images and different cellular organelles. After comparing the parameters in cellular organelles with these in 6 stages, the whole endocytosis pathway was demonstrated. These results verified that this probe successfully tracked the whole intracellular dynamic endocytosis process of phenylephrine. Our method realized the visual tracking the whole receptor-mediated endocytosis, which is a new approach on investigating the molecular mechanisms and kinetic properties of intracellular receptor-drug transportation.     

前列腺癌基因3的研究进展

前列腺癌的早期诊断、鉴别诊断目前仍面临许多挑战。前列腺癌基因3（PCA3）为近年来发现的一种前列腺癌组织高特异性标记物,引起了国内外学者的关注。本文就其分子生物学特性、以及在前列腺癌诊断、治疗中的应用进展进行综述。     

The “neurovascular unit approach” to evaluate mechanisms of dysfunctional autoregulation in asphyxiated newborns in the era of hypothermia therapy

Despite improvements in obstetrical and neonatal care, and introduction of hypothermia as a neuroprotective therapy, perinatal brain injury remains a frequent cause of cerebral palsy, mental retardation and epilepsy. The recognition of dysfunction of cerebral autoregulation is essential for a real time measure of efficacy to identify those who are at highest risk for brain injury.     

五倍子水提取物对人牙髓细胞Ⅰ型胶原合成的影响

目的:观察五倍子水提取物对人牙髓细胞Ⅰ型胶原合成的影响。方法:取第6代体外培养的人牙髓细胞分别与终末浓度为5、10μg/m L的五倍子水提取物共同培养3 d后,采用免疫组化染色法观察人牙髓细胞中Ⅰ型胶原的表达情况,并用HPIAS-1000图像分析系统进行定量分析。结果:对照组和不同浓度五倍子水提取物作用的实验组人牙髓细胞中均有Ⅰ型胶原的表达,其免疫反应物质多定位于胞浆。人牙髓细胞经5、10μg/m L浓度的五倍子水提取物作用后,其Ⅰ型胶原的表达水平均明显增高,其中以10μg/m L组的增高程度最大,各组间两两相比差异均有统计学意义(P<0.05)。结论:5、10μg/m L五倍子水提取物均能明显促进人牙髓细胞中Ⅰ型胶原的表达;提示其具有促进牙髓细胞向成牙本质细胞分化的作用。     

Polymorphism in miRNA-1 target site and circulating miRNA-1 phenotype are associated with the decreased risk and prognosis of coronary artery disease

MiRNA molecules have been identified to play key roles in a broad range of physiologic and pathologic processes. Polymorphisms in microRNA target sites (PolymiRTSs) can disturb or obstruct miRNA binding and consequentially influence regulation of the target genes. A two-step study design was used in this study. A case-control study was designed to assess the relationship between miRNA-1 target site rs9548934C→T polymorphism in target gene (Component of Oligomeric Golgi Complex 6, COG6) and risk of coronary artery disease (CAD) in 1013 patients and 610 normal controls. This genetic variant was also evaluated for the association with major adverse cardiovascular events (MACE) of CAD in a follow-up study, including 785 (785/1013) patients followed up for 42 months. The phenotypes of circulating miRNA-1 levels in 34 cases were slightly lower than that of 40 controls but not significantly different (P = 0.090). The CT and CT/TT genotypes were associated with a 34% and 26% decreased risk of CAD, and the TT and CT/TT genotypes were associated with a 76% and 49% decreased risk of MACE of CAD. Cox regression analysis showed that rs9548937 C/T variant was associated with a decreased risk of MACE, while age, diabetes mellitus, higher levels of CRP (≥ 3.80 mg/L) and three pathological changes in the coronary artery were associated with an increased risk of MACE. Our findings implicate miRNA-1 target site rs9548934C→T genotypes, circulating miRNA-1 phenotype and CRP levels may modulate the occurrence and MACE of CAD.     

后牙游离缺失患者对5种修复方法的主观评价

目的了解后牙游离缺失患者对种植义齿、单端固定桥、附着体义齿、活动义齿、短牙弓5种处理方法的主观评价。方法对133例后牙游离缺失患者,通过问卷记录患者的基本情况以及患者选择修复方法时考虑的主要因素,用图片及文字说明让患者充分了解各种修复方法的优缺点,在视觉模拟尺上标记患者对5种方法的评分。分析患者的基本情况与主观评价之间的关系。结果 133例患者对种植义齿、单端固定桥、附着体义齿、活动义齿、短牙弓的评分分别为56.5±20.1、40.2±17.9、34.9±15.4、48.1±19.4、36.6±15.2。患者对修复方法的评价受年龄、教育水平、牙列现状等因素的影响,差异有统计学意义（P〈0.05）。结论患者对种植义齿的主观评价最高,对短牙弓的评价最低,患者对不同修复方法的评价与自身情况有关。     

种植牙术后局部应用盐酸米诺环素的研究

目的：探讨盐酸米诺环素在牙种植术后的应用效果。方法：对58例患者,共80枚种植体,随机分成两组,实验组30例,共40枚种植体,外科植入后于种植体周围推注盐酸米诺环素软膏,术后1周、2周、3周再次应用盐酸米诺环素软膏;对照组28例,共40枚种植体,常规给于克林霉素72小时,然后改用复方氯己定漱口水含漱。术后4周、12周,比较菌斑指数（PLI）、种植体周改良出血指数（mBI）、探诊深度（PD）,比较两组种植牙成功率。数据用PASW statistic 18.0软件进行统计学分析。结果：对种植体成功率的影响两组比较无显著差异。结论：术后种植体周围应用盐酸米诺环素可替代全身应用抗生素预防种植术后早期感染。