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21.
Leung H Hughes C Kloppel G Williamson R Lemoine N 《International journal of oncology》1994,4(6):1219-1223
Growth factors may play a role in autocrine or paracrine growth control of tumour cells. We have now examined the expression pattern in vivo by in situ hybridization (ISH) on a series of 13 ductal adenocarcinomas of the pancreas using the non-radioactive digoxigenin system to generate specific antisense orientated riboprobes for FGF-1 and FGF-2, and the four FGFRs (FGFR-1, -2, -3 and -4). We confirmed the expression of both FGF/FGFR by tumour cells, with the potential of a potential autocrine loops in 46% of the cases studied. FGF-2 and FGFR-3 were the most commonly expressed ligand and receptor (46% and 76% respectively). Endothelial cells lining vessels within an around invasive tumours were frequently positive for expression of FGFR-1 and/or FGFR-3. In the normal pancreas remote from the tumour, the acinar cells were found to have a heterogeneous expression pattern for FGFRs while duct cells, islet cells and stromal components including nerves and vascular endothelium were negative. The data suggest a role for FGFs and their high affinity receptors in the control of growth of human pancreatic adenocarcinoma and its supporting stroma. 相似文献
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Heautot JF Eichelberg M Gibaud B Tréguier C Lemoine D Scarabin JM Piqueras J Carsin M Gandon Y 《European radiology》2000,10(1):175-182
The RETAIN project (Radiological Examinations Transfer on an ATM Integrated Network) has aimed at testing videoconferencing
and DICOM image transfers to get advice about difficult radiological cases over an asynchronous transfer mode (ATM)-based
network, which affords a more comfortable interface than narrow-band networks and allows exchange of complete image series
using the DICOM format of studies. For this purpose, an experimental ATM network was applied between six university hospitals
in four different countries. An assessment of the functionalities of the system was performed by means of log-file analysis,
video recording of the sessions and forms filled out by the participants at the end of each session. Questionnaires were answered
by the users at the end of the project to bring out perspectives of utilisation and added value. We discussed 43 cases during
20 sessions. For technical or organisational problems, only 20 of the 36 planned sessions took place. The throughput over
ATM (10.5 Mbit/s, 20 times faster than six ISDN B-channels) was adequate. Despite the experimental configuration of the network,
the system was considered as satisfactory by all the physicians. In 72 % of the sessions, the expected result (answer to the
question) was gained. By common consent, videoconferencing was unanimously regarded as a prominent tool in improving the interaction
quality. Asynchronous transfer mode is an efficient method for fast transferring of radiologic examinations in DICOM format
and for discussing them through high-quality videoconferencing.
Received: 26 October 1998; Revised: 25 February 1999; Accepted: 2 April 1999 相似文献
24.
Burgel JS Navarro F Lemoine MC Michel J Carabalona JP Fabre JM Domergue J 《Annales de chirurgie》2000,125(3):231-237
OBJECTIVE: The aim of this prospective study was to assess the feasibility and postoperative advantages of the laparoscopic-assisted elective colectomy for diverticular disease. PATIENTS AND METHODS: From january 1989 to december 1997, among the 114 patients electively operated on for diverticulitis, 56 patients were treated by laparoscopic approach. Evaluated parameters included: gender, age, weight, size, ASA score, operating time, duration of hospital stay, of analgesic treatment, and of postoperative ileus, morbidity and mortality rate. RESULTS: The study group consisted of 35 women and 21 men. Mean age was 59 years (34-81 years); 29 patients were ASA 1 and 27 ASA 2. Overall postoperative mortality rate was 0% and morbidity rate 16% (n = 9). There were no complications directly related to laparoscopic technique. The conversion rate was 14% (n = 8). Mean operating time was 300 min (200-600 min). Mean duration of postoperative ileus was 2.4 days. Mean duration of hospital stay was 9.4 days. CONCLUSION: This study demonstrates the feasibility of elective laparoscopic-assisted colonic resection for diverticular disease in more than 80% of cases with a postoperative morbidity and mortality rate comparable to those of conventional surgery. 相似文献
25.
双波长薄层扫描法测定痛得安胶囊中新乌头碱的含量 总被引:3,自引:0,他引:3
目的:建立痛得安胶囊中新乌头碱的含量测定方法。方法:采用双波长薄层扫描法,以正己烷-乙酸乙酯-无水乙醇-氨水(12:8:2.5:0.5)为展开剂,碘化饿钾试液为显色剂,测定该制剂中新乌头碱的含量。结果:线性范围为1~6ug。平均回收率为94.80%,RSD为2.03%。结论:本法操作简便。结果可靠。实用,适合该制剂中新乌头碱的含量测定。 相似文献
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Sandrine Lemoine René Rouet Alain Manrique Jean‐Luc Hanouz 《Fundamental & clinical pharmacology》2014,28(5):481-488
Lipid emulsions are used in the reversal of local anesthetic toxicity. The aim of this study was to investigate the cellular electrophysiological effects of long‐chain triglyceride lipid emulsion (LCTE) on cardiac action potential characteristics and conduction disturbances induced by bupivacaine. Purkinje fibers were dissected from the left ventricle of New Zealand white rabbit hearts and superfused with either Tyrode's solution during 30 min (control group), with bupivacaine 10?6 m , 10?5 m , and 5.10?5 m alone, or in the presence of LCTE 0.5%, in addition, LCTE at 0.1%, 0.5%, and 1% was perfused alone. Electrophysiological parameters were recorded using the conventional microelectrode technique (37 °C, 1 Hz frequency). Bupivacaine 5.10?5 m ‐induced conduction blocks (8/8 preparations): LCTE 0.5% suppressed the bupivacaine 5.10?5 m ‐induced conduction blocks (1/8 preparations). Exposure to bupivacaine 10?6 m , 10?5 m , and 5.10?5 m resulted in a significant decrease in the maximal rate of depolarization (Vmax) (respectively, 25%, 55%, 75%; P < 0.002 vs. control group). In the presence of LCTE 0.5%, bupivacaine 10?6 m did not significantly decreased Vmax (13%; P = 0.10 vs. control group). The decrease in Vmax resulting from bupivacaine 10?5 m alone was significantly less in the presence of LCTE 0.5% (P < 0.01 vs. bupivacaine 10?5 m alone). Exposure to bupivacaine 10?6 m , 10?5 m , and 5.10?5 m alone or in the presence of LCTE 0.5% resulted in a significant decrease in action potential duration measured at 50% and 90% repolarization (APD50 and APD90; P < 0.01 vs. control group). LCTE inhibited the Purkinje fibers conduction blocks induced by bupivacaine. Moreover, LCTE 0.5% attenuates the decrease in Vmax induced by bupivacaine 10?6 m and 10?5 m . 相似文献
29.
Stijn JB Van Weyenberg Sietze T Van Turenhout Maarten AJM Jacobs Gerd Bouma Chris JJ Mulder 《Digestive endoscopy》2012,24(4):247-254
Background and Aim: Little is known about the causes of overt obscure gastrointestinal bleeding (OGIB) in patients using anti‐thrombotic therapy. We aimed to describe video capsule endoscopy (VCE) findings and to identify factors associated with positive findings in these patients. Methods: We carried out a retrospective study of 56 patients who underwent VCE for evaluation of previous overt OGIB during anti‐thrombotic therapy. VCE studies were re‐evaluated by a gastroenterologist blinded to clinical details. Clinical data included in the multivariate analysis were sex, age, indication for and type of anti‐thrombotic therapy, hemodynamic instability on admission, type of blood loss, hemoglobin on admission, use of a proton pump inhibitor, NSAID use, time between bleeding episodes and VCE, and whether or not anti‐thrombotic therapy was resumed before the VCE study. Results: A probable cause for gastrointestinal bleeding was identified in 28 (50%) of the 56 studies. Angiodysplasia was found in 19 patients. Twenty‐two studies showed a possible cause in the small bowel. Multivariate logistic regression analysis showed that reinstitution of anti‐thrombotic therapy before VCE was carried out was the only independent predictor of positive VCE findings (OR: 8.61, 95% CI: 1.20–60.42, P = 0.032). Conclusions: Small intestinal angiodysplasia was the most common cause for overt OGIB. Reinstitution of withdrawn anti‐thrombotic drugs before the VCE examination was carried out was associated with positive VCE findings in multivariate analysis. 相似文献
30.
The tetrapeptide Acetyl-N-Ser-Asp-Lys-Pro (AcSDKP, Seraspenide; Ipsen- Biotech, Paris, France), an inhibitor of murine spleen colony-forming units reduces the number and the percentage in DNA synthesis of progenitors from human unfractionated bone marrow. To determine whether AcSDKP may directly affect the growth potential of purified progenitors even at the most primitive level, CD34+HLA-DRhigh and CD34++HLA-DRlow cells were highly purified by cell sorting. Then, CD34+ subsets were stimulated in liquid culture with combinations of growth factors (GFs) and AcSDKP was added for 20 hours or 6 days and cells plated in methylcellulose. After a 20-hour incubation, we show that AcSDKP (at 10(-10) mol/L) significantly inhibits the colony formation of both CD34+ subsets. Moreover, when added daily for 6 days, AcSDKP: (1) reduces the proliferation of both CD34+ cell fractions stimulated by 3 or 7 GFs, and (2) decreases the number of progenitors generated from the CD34+HLA-DRhigh and CD34++HLA-DRlow cell fractions. Furthermore, we show for the first time, using both high proliferative potential cell and long-term culture initiating cell assays, that AcSDKP inhibits the most primitive cells contained in the CD34++HLA-DRlow subpopulation. Finally, by using limiting dilution assays we demonstrated that AcSDKP acts directly at a single cell level and that its inhibitory effect is reversible and dose dependent. 相似文献