全文获取类型
收费全文 | 2102篇 |
免费 | 131篇 |
国内免费 | 12篇 |
专业分类
耳鼻咽喉 | 32篇 |
儿科学 | 79篇 |
妇产科学 | 53篇 |
基础医学 | 257篇 |
口腔科学 | 102篇 |
临床医学 | 138篇 |
内科学 | 481篇 |
皮肤病学 | 39篇 |
神经病学 | 128篇 |
特种医学 | 26篇 |
外国民族医学 | 1篇 |
外科学 | 299篇 |
综合类 | 16篇 |
一般理论 | 1篇 |
预防医学 | 252篇 |
眼科学 | 42篇 |
药学 | 149篇 |
中国医学 | 12篇 |
肿瘤学 | 138篇 |
出版年
2023年 | 17篇 |
2022年 | 37篇 |
2021年 | 70篇 |
2020年 | 38篇 |
2019年 | 61篇 |
2018年 | 87篇 |
2017年 | 58篇 |
2016年 | 64篇 |
2015年 | 96篇 |
2014年 | 108篇 |
2013年 | 98篇 |
2012年 | 155篇 |
2011年 | 186篇 |
2010年 | 94篇 |
2009年 | 89篇 |
2008年 | 148篇 |
2007年 | 142篇 |
2006年 | 126篇 |
2005年 | 96篇 |
2004年 | 83篇 |
2003年 | 71篇 |
2002年 | 57篇 |
2001年 | 31篇 |
2000年 | 38篇 |
1999年 | 24篇 |
1998年 | 9篇 |
1997年 | 6篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1992年 | 9篇 |
1991年 | 13篇 |
1990年 | 10篇 |
1989年 | 7篇 |
1988年 | 8篇 |
1987年 | 10篇 |
1986年 | 15篇 |
1985年 | 6篇 |
1984年 | 12篇 |
1982年 | 2篇 |
1980年 | 5篇 |
1977年 | 2篇 |
1974年 | 4篇 |
1972年 | 2篇 |
1971年 | 4篇 |
1970年 | 3篇 |
1969年 | 2篇 |
1968年 | 2篇 |
1967年 | 5篇 |
1966年 | 5篇 |
1965年 | 4篇 |
排序方式: 共有2245条查询结果,搜索用时 13 毫秒
71.
72.
Shimada A Takahashi Y Muramatsu H Hama A Ismael O Narita A Sakaguchi H Doisaki S Nishio N Tanaka M Yoshida N Matsumoto K Kato K Watanabe N Kojima S 《International journal of hematology》2012,95(6):675-679
Fanconi anemia (FA) is a disorder characterized by developmental anomalies, bone marrow failure and a predisposition to malignancy. It has recently been shown that hematopoietic stem cell transplantation using fludarabine (FLU)-based reduced-intensity conditioning is an efficient and quite safe therapeutic modality. We retrospectively analyzed the outcome of bone marrow transplantation (BMT) in eight patients with FA performed in two institutes between 2001 and 2011. There were seven females and one male with a median age at diagnosis = 4.5 years (range 2-12 years). The constitutional characteristics associated with FA, such as developmental anomalies, short stature and skin pigmentation, were absent in three of the patients. One patient showed myelodysplastic features at the time of BMT. All patients received BMT using FLU, cyclophosphamide (CY) and rabbit anti-thymocyte globulin (ATG) either from a related donor (n = 4) or an unrelated donor (n = 4). Acute graft-versus-host disease (GVHD) of grade I developed in one patient, while chronic GVHD was not observed in any patient. All patients are alive and achieved hematopoietic recovery at a median follow-up of 72 months (range 4-117 months). BMT using FLU/low-dose CY/ATG -based regimens regardless to the donor is a beneficial therapeutic approach for FA patients. 相似文献
73.
Fuster O Barragán E Bolufer P Such E Valencia A Ibáñez M Dolz S de Juan I Jiménez A Gómez MT Buño I Martínez J Cervera J Montesinos P Moscardó F Sanz MÁ 《Annals of hematology》2012,91(1):1-7
During last years, molecular markers have been increased as prognostic factors routinely screened in acute myeloid leukemia (AML). Recently, an increasing interest has been reported in introducing to clinical practice screening for mutations in the CCAAT/enhancer-binding protein ?? (CEBPA) gene in AML, as it seems to be a good prognostic factor. However, there is no reliable established method for assessing CEBPA mutations during the diagnostic work-up of AMLs. We describe here a straightforward and reliable fragment analysis method based in PCR capillary electrophoresis (PCR-CE) for screening of CEBPA mutations; moreover, we present the results obtained in 151 intermediate-risk karyotype AML patients (aged 16?C80?years). The method gave a specificity of 100% and sensitivity of 93% with a lower detection limit of 1?C5% for CEBPA mutations. The series found 19 mutations and four polymorphisms in 12 patients, seven of whom (58%) presented two mutations. The overall frequency of CEBPA mutations in AML was 8% (n?=?12). CEBPA mutations showed no coincidence with FLT3-ITD or NPM1 mutations. CEBPA mutation predicted better disease-free survival in the group of patients without FLT3-ITD, NPM, or both genes mutated (HR 3.6, IC 95%; 1.0?C13.2, p?=?0.05) and better overall survival in patients younger than 65 of this group without molecular markers (HR 4.0, IC 95%; 1.0?C17.4, p?=?0.05). In conclusion, the fragment analysis method based in PCR-CE is a rapid, specific, and sensitive method for CEBPA mutation screening and our results confirm that CEBPA mutations can identify a subgroup of patients with favorable prognosis in AML with intermediate-risk karyotype. 相似文献
74.
75.
Luis Antonio dos Santos Diego Christiane D'Oliveira Marques Pedro Thadeu Galvão Vianna Rosa Marlene Viero José Reinaldo Cerqueira Braz 《Renal failure》2013,35(8):1039-1045
Introduction. Hypovolemia from hemorrhage evokes protective compensatory reactions, such as the renin-angiotensin system, which interferes in the clearance function and can lead to ischemia. This study was designed to evaluate the effects of glibenclamide, a K+ATP channel blocker, on renal function and histology in rats in a state of hemorrhagic shock under sevoflurane anesthesia. Material and Methods. Twenty Wistar rats were randomized into two groups of 10 animals each (G1 and G2), only one of which (G2) received intravenous glibenclamide (1 μg.g?1), 60 min before bleeding was begun. Both groups were anesthetized with sevoflurane and kept on spontaneous respiration with oxygen-air, while being bled of 30% of volemia in three stages with 10 min intervals. There was an evaluation of renal function—sodium para-aminohippurate and iothalamate clearances, filtration fraction, renal blood flow, renal vascular resistance—and renal histology. Renal function attributes were evaluated at three moments: M1 and M2, coinciding with the first and third stages of bleeding; and M3, 30 min after M2, when the animals were subjected to bilateral nephrectomy before being sacrificed. Results. Significant differences were found in para-aminohippurate clearance, G1 < G2, and higher renal vascular resistance values were observed in G1. Histological examination showed the greater vulnerability of kidneys exposed to sevoflurane alone (G1) with higher scores of vascular and tubular dilatation. There were vascular congestion and tubular vacuolization only in G1. Necrosis and signs of tubular regeneration did not differ in both groups. Conclusion. Treatment with glibenclamide attenuated acutely the renal histological changes after hemorrhage in rats under sevoflurane anesthesia. 相似文献
76.
Alejandro Balestracci Sandra Mariel Martin Ismael Toledo Caupolican Alvarado Raquel Eva Wainsztein 《Pediatric nephrology (Berlin, Germany)》2013,28(6):919-925
Background
Platelet transfusions should be avoided in children with post-diarrheal hemolytic uremic syndrome (D + HUS) because they might increase microthrombi formation, thereby aggravating the disease. As this possibility has not yet been explored, we investigated whether platelet transfusion in patients with D + HUS would lead to a worse disease course compared to that in patients who did not receive platelet transfusion.Methods
This was a case–control study in which data from D + HUS children who received platelet transfusions (cases, n? = ?23) and those who did not (controls, n? = ?54) were retrospectively reviewed and compared.Results
Both patient groups were similar in age (p?=?0.3), gender (p? =? 0.53), weight (p? = ?0.86), height (p? = ?0.45), prior use of non-steroidal anti-inflammatory drugs (p? = ?0.59) or antibiotics (p ?= ?0.45) and presence of dehydration at admission (p? = ?0.79). The two groups also did not differ in initial leukocyte count (p? = ?0.98), hematocrit (p? = ?0.44) and sodium (p? = ?0.11) and alanine aminotransferase levels (p? = ?0.11). During hospitalization, dialysis duration (p? = ?0.08), number of erythrocyte transfusions (p? =? 0.2), serum creatinine peak (p? = ?0.22), presence of severe bowel (p? = ?0.43) or neurologic (p? = ?0.97) injury, arterial hypertension (p? = ?0.71), need for intensive care (p? = ?0.33) and death (p? = ?1.00) were also comparable.Conclusion
Our findings suggest that platelet transfusion does not aggravate the course of the disease. Conversely, no hemorrhagic complications were observed in the group of patients who did not receive a platelet transfusion. Until these observations are confirmed by further studies, the benefits and risk of platelet transfusion should be thoughtfully balanced on an individual case basis. 相似文献77.
78.
Jos-Ramn Blanco Ramn Morillo Vicente Abril Ismael Escobar Enrique Bernal Carlos Folguera Ftima Braas Mercedes Gimeno Olatz Ibarra Jos-Antonio Iribarren Alicia Lzaro Ana Mario Mara-Teresa Martn Esteban Martinez Luis Ortega Julian Olalla Aguas Robustillo Matilde Sanchez-Conde Miguel-Angel Rodriguez Javier de la Torre Javier Sanchez-Rubio Montse Tuset 《European journal of clinical pharmacology》2020,76(3):305-318
79.
The pH 6 antigen (Psa) of Yersinia pestis consists of fimbriae with adhesive properties of potential importance for the pathogenesis of plague, including pneumonic plague. The Psa fimbriae mediate bacterial binding to human alveolar epithelial cells. The Psa fimbriae bound mostly to one component present in the total lipid extract from type II alveolar epithelial cells of the cell line A549 separated by thin-layer chromatography (TLC). The Psa receptor was identified as phosphatidylcholine (PC) by TLC using alkali treatment, molybdenum blue staining, and Psa overlays. The Psa fimbriae bound to PC in a dose-dependent manner, and binding was inhibited by phosphorylcholine (ChoP) and choline. Binding inhibition was dose dependent, although only high concentrations of ChoP completely blocked Psa binding to PC. In contrast, less than 1 muM of a ChoP-polylysine polymer inhibited specifically the adhesion of Psa-fimbriated Escherichia coli to PC, and type I (WI-26 VA4) and type II alveolar epithelial cells. These results indicated that the homopolymeric Psa fimbriae are multimeric adhesins. Psa also bound to pulmonary surfactant, which covers the alveolar surface as a product of type II alveolar epithelial cells and includes PC as the major component. The observed dose-dependent interaction of Psa with pulmonary surfactant was blocked by ChoP. Interestingly, surfactant did not inhibit Psa-mediated bacterial binding to alveolar cells, suggesting that both surfactant and cell membrane PC retain Psa-fimbriated bacteria on the alveolar surface. Altogether, the results indicate that Psa uses the ChoP moiety of PC as a receptor to mediate bacterial binding to pulmonary surfactant and alveolar epithelial cells. 相似文献
80.
Taurine, an inducer for tau polymerization and a weak inhibitor for amyloid-beta-peptide aggregation
Taurine is an abundant aminoacid present in brain. Its concentration is decreased in the brain of Alzheimer's disease (AD) patients. The chemical structure of taurine is similar to 3-amino-1-propanesulfonic acid, a known compound which interferes with beta-amyloid peptide aggregation. Here, we have tested if taurine show similar properties. Taurine slightly decreases beta-amyloid peptide aggregation at a milimolar concentration. At that concentration, taurine favours the assembly of tau protein into fibrillars polymers. Thus, it is proposed that the negative charge present in taurine may be involved in the binding to tau protein, facilitating its assembly. In addition, the possible role of taurine in Alzheimer disease is commented. 相似文献