Cerebrospinal fluid zinc concentrations in febrile convulsions

Zinc modulates the activity of glutamic acid decarboxylase, the rate limiting enzyme in the synthesis of gamma-aminobutyric acid (GABA), which is a major inhibitory neurotransmitter. Low cerebrospinal fluid GABA values have been reported in association with several seizure disorders, including febrile convulsions. It is also known that fever and/or infections may cause a reduction in serum zinc concentrations. In this study the hypothesis that febrile convulsions are related to low cerebrospinal fluid zinc was tested. Cerebrospinal fluid zinc concentrations were measured in 66 febrile children: 32 with febrile convulsions, 18 with fever but without convulsions, and 16 with aseptic (viral) meningitis. There was no statistically significant difference in the cerebrospinal fluid zinc between the three groups of children, and the mean concentration was 26.2 micrograms/l. No significant relationship was found between either age, gender, maximal temperature, type of infection, or time of performance of the lumbar puncture and cerebrospinal fluid zinc concentration. These results do not support the hypothesis that febrile convulsions are related to reduced cerebrospinal fluid zinc concentrations.     

程序升温法研究药物稳定性的试验误差

通过电子计算机模拟程序升温加速试验数据,从理论上探讨了各种程序升温法的试验误差,并与恒温法作了对比。结果表明,药物降解程度越大,试验温度差越大,平均温度越接近室温,抽样频率越高,试验结果误差就越小。在药物降解程度和加热温度相同的条件下,指数程序升温法的试验误差明显小于线性升温、倒数升温和对数升温法。恒温法的试验结果误差小于指数升温法,但所需的加热时间约为指数升温法的5倍。     

静脉注射同种异体骨髓间充质干细胞对血管性痴呆大鼠海马区脑组织形态及微管相关蛋白2表达的影响

目的:骨髓间充质干细胞移植进入脑组织后,能否影响脑组织的形态及微管相关蛋白2的表达从而改善痴呆状态下的认知功能?观察静脉注射同种异体骨髓间充质干细胞后,血管性痴呆模型大鼠脑海马CA1区脑组织形态及微管相关蛋白2的变化。方法:实验于2004-08/2005-05在解放军第二军医大学完成。①实验动物:健康雄性SD大鼠60只,随机数字表法分为细胞移植组、模型对照组、假手术组,20只/组。②实验方法:另取20只大鼠用于体外分离、培养扩增骨髓间充质干细胞,传至第2代时加入BrdU进行标记,制备单细胞悬液,调整细胞浓度为3×109L-1。细胞移植组、模型对照组采用双侧颈总动脉结扎法建立血管性痴呆动物模型,假手术组仅暴露双侧颈总动脉但不结扎。造模后4周,细胞移植组尾静脉注射骨髓间充质干细胞悬液1mL,模型对照组注射等量磷酸盐缓冲液,假手术组不进行尾静脉注射。③实验评估:细胞移植后4周,苏木精-伊红染色检测各组大鼠脑海马CA1区脑组织形态变化,免疫荧光染色观察经BrdU标记的骨髓间充质干细胞示踪情况,免疫组织化学染色检测脑海马CA1区微管相关蛋白2的表达。结果:细胞移植组5只大鼠死亡,模型对照组3只大鼠死亡,假手术组均进入结果分析。①脑海马CA1区锥体细胞形态变化:细胞移植组和模型对照组CA1区锥体细胞较假手术组排列稀疏、紊乱,细胞肿胀、脱失,核固缩,但细胞移植组细胞排列较模型对照组规则,且细胞肿胀、脱失及核固缩较模型对照组减轻。②脑海马内骨髓间充质干细胞的示踪:细胞移植后4周,大鼠脑海马内可见被绿色荧光标记的骨髓间充质干细胞。③脑海马CA1区微管相关蛋白2的表达:与假手术组比较,细胞移植组及模型对照组脑海马CA1区锥体细胞层微管相关蛋白2阳性产物的吸光度值均明显降低(P<0.05);细胞移植组明显高于模型对照组(P<0.05)。结论:静脉注射骨髓间充质干细胞能够使血管性痴呆大鼠脑海马CA1区神经元损伤及脱失减轻,并使微管相关蛋白2表达增加。     

彩色室壁运动和定量分析软件观测腹主动脉瘤壁的节段运动

目的：运用彩色室壁运动定量分析正常腹主动脉壁及腹主动脉瘤患者瘤壁运动的可行性。 方法：于2006-04/12选择解放军第二军医大学长海医院血管外科收治的肾动脉下型腹主动脉瘤患者10例,均为男性,预行腹主动脉瘤人工血管置换术或腔内隔绝术瘤体直径均大于4.5cm,为腹主动脉瘤组。同期选择健康体检年轻男性20例为正常年轻组和健康老年男性20例为正常老年组。3组受试者年龄、血压比较无显著差异,具有可比性。实验经医院伦理委员会批准,所有受试者均知情同意。使用彩色室壁运动技术对正常年轻组及正常老年组受试者肾动脉下腹主动脉壁运动和腹主动脉瘤组患者动脉瘤壁运动进行观测和记录,并使用定量分析软件（ICKTM）对所得图像加以分析。正常年轻组和正常老年组受检者均测量脐上1cm处腹主动脉的横及纵切面的动脉壁的运动参数。腹主动脉瘤组患者通过超声测量腹主动脉瘤入口、最大内径处和出口处的内径及壁厚。 结果：①动脉壁位移幅度：正常年轻组、正常老年组、腹主动脉瘤组各点上的前壁位移均较后壁位移大（P〈0.05）。正常老年组腹主动脉前壁位移距离较正常年轻组小（P〈0.05）,后壁位移距离与正常年轻组无显著差异（P〉0.05）。腹主动脉瘤组前后壁的位移在入口、最大直径处、出口均较正常老年组前后壁位移小（P〈0.05）。腹主动脉瘤入口处前壁和后壁的位移距离均较最大直径处和出口处大（P〈0.05）。②正常腹主动脉和腹主动脉瘤壁的运动情况：正常年轻组腹主动脉壁的运动是基本对称和一致的,即在横切面上各节段是同时完成收缩和舒张的。腹主动脉瘤壁在横切面上的运动则失去了这种对称性和一致性,特别是在舒张期。正常年轻组纵切面,前壁位移幅度明显较后壁大,腹主动脉瘤组纵切面,后壁基本没有移动,前壁的位移也不均一。③定量分析软件（ICKTM）结果：正常腹主动脉壁各段的运动虽速度不一样,但基本是同时达到最大位移。腹主动脉瘤壁各段的运动不一致,完成位移的时间均不相同。 结论：使用彩色室壁运动技术和定量分析软件（ICKTM）观测腹主动脉和腹主动脉瘤壁节段运动是可行的,可以对瘤壁运动提供更多的信息。     

Acute haemorrhagic oedema of infancy, a benign variant of leucocytoclastic vasculitis

Acute haemorrhagic oedema of infancy (AHEI) is an acute leucocytoclastic vasculitis affecting infants and young children. It has a striking appearance of large purpuric skin lesions in a target-like pattern and marked oedema mainly on the face, auricles and extremities. In some patients there is mucosal involvement as well. We present five patients with skin lesions characteristic of AHEI with no visceral involvement and complete resolution within 7-14 days. In three of our five cases, histopathological examination was performed, and demonstrated typical leucocytoclastic vasculitis. Although sometimes confused with Schönlein- Henoch purpura, we suggest that AHEI should be regarded as a separate entity. Clinical criteria for diagnosis are proposed.     

维生素C片剂分解动力学研究

在相对湿度小于临界相对湿度的条件下,在温度为60～90℃、相对湿度为60～80%范围内测定了维生素C片剂的色泽(以相对反射率R表示)变化速率,含量C变化速率及片剂溶液的色泽(以透光率T表示)变化速率。三者的表观速率常数k与热力学温度T和相对湿度RH的关系通式为k=k’exp(-E/RT)exp(mRH),k’和m为常数,E为表观活化能。不同速率常数的k’,m和E值均不同。     

维生素C粉末分解动力学研究

在有氧避光条件下,用恒温恒湿加速试验法,研究了在相对湿度小于临界相对湿度时,维生素C粉末的表观变色速率常数k_c与温度、湿度的关系为:k_c=k′exp(-E_a/RT)exp(m′RH),k′与m′为常数,RH为相对湿度,E_a为活化能。RH<70%时的E_a=104.33 kJ·mol^-1。还研究了在各种相对湿度下维生素C粉末含量变化的曲线方程为:C=N/(1+Btⁿ),速率方程为:k_d为分解反应的表现速率常数,N为原始百分含量,C为时间t的含量,B与n为常数。RH为97,93与79.5%时的E_a分别为86.87,88.22与202.64 kJ·mol^-1。     

角质的免疫组织化学定位在头颈肿瘤病理论断中的应用

本研究报告100例头颈正常及肿瘤组织石蜡切片的角质抗体定位。结果表明上呼吸、消化道之被覆上皮、涎腺导管、耵聍腺及中耳上皮、毛囊及汗腺等上皮组织均阳性,而间叶组织则为阴性。鳞癌、基底细胞癌等上皮源性肿瘤均为阳性。再次肯定了角质抗体定位的诊断价值。     

Clinical Effects of Lithospermum Ruderale Dosage and Using-time on Medicinal Abortion Induced by Mifepristone and Misoprostol

Medicinal abortion with mifepristone and misoprostol has been widely used andaccepted in clinical practice. However,there are still some shortcomings such as thecomparatively highly incomplete abortion rate( 5～ 7% ) and long-term vaginal bleed-ing[1 ,2 ] .We have used Lithospermum Ruderale in medicinal abortion,which wasfound,to some extent,to be able to increase the complete abortion rate and shortenthe vaginal bleeding time[3 ] . In order to approach the appropriate and reasonabledosage an…