两种不同方法治疗股骨头缺血坏死的实验研究

目的 观察股骨头坏死的病理变化。观察带血运骨瓣移植术与带血运骨瓣与骨膜生发层细胞复合移植术的疗效。比较两种治疗方法之间是否有差别。探讨血供重建与骨重建相结合的可能性。方法 ２２只哈白兔分为３组。均将股骨头截下后埋于胸部皮下,制成股骨头坏死模型。３周后,Ａ组对照组６只,无处置;Ｂ组８只,将带血运肋骨瓣植入已坏死的股骨头内,Ｃ组８只,将带血运肋骨瓣与骨膜生发层细胞复合植入已坏死的股骨头内,１２周后,光镜、透射电镜观察股骨头的软骨及骨小梁的结构。结果 Ａ组出现典型骨坏死而无修复,Ｂ组出现成骨细胞及红细胞,修复反应出现,Ｃ组出现修复反应,且修复效果优于Ｂ组。结论 应用哈白兔将股骨头截古后埋藏于皮下,可以制作出 血运肋骨诱发了骨重建,从而加快坏死骨修复,实验性血供重建与骨重建复合移植组对骨坏死的修复优于单纯血供重建组。     

电针肾俞、膈俞、百会穴对拟血管性痴呆学习记忆障碍小鼠水迷宫实验的影响

目的　观察电针肾俞、膈俞、百会穴对拟血管性痴呆小鼠学习记忆障碍的影响。方法　复制脑缺血再灌注拟血管性痴呆小鼠模型 ,并电针肾俞、膈俞、百会穴 ,分别于术后 7天、15天、30天 ,与药物喜德镇对照 ,采用水迷宫法 ,记录游全程时间、错误次数。结果　造模导致了小鼠学习与记忆能力下降 ,表现为游全程时间延长 ,错误次数增加 ,且随着观察时间的延长 ,这种改变逐渐加重。电针和喜德镇均可使模型小鼠的游全程时间缩短 ,错误次数减少 ,但电针组于 7天和 30天时 ,成绩显著优于药物组。结论　电针肾俞、膈俞、百会穴对模型小鼠学习与记忆能力下降有改善和提高作用     

异氟醚分娩镇痛对母体及胎儿母体循环中cGMP水平的影响

目的;探讨镇痛浓度的异氟醚对母体及胎儿－母体循环中环磷酸鸟苷水平的影响。     

贲门癌患者围手术期上消化道动力学观察

目的观察贲门癌对食管运动功能的影响以及手术后残余食管、残胃的运动功能改变。方法　用低顺应性三腔聚乙烯液压毛细管灌注导管对22例正常人和31例中晚期贲门癌患者进行了食管压力测定。所有受检者均仰卧位,每次吞咽5ml水,采用定点牵拉法。计算机自动计算出各部位的静息压、收缩压、收缩及松弛时间、传播速度。结果贲门癌患者手术前与正常人的各项指标(除下括约肌外)相比较,贲门癌患者食管下段收缩时间明显长于正常人。贲门癌患者手术前后的数据比较:①手术后上括约肌收缩压明显高于手术前。②手术后食管上段静息压和收缩压均明显高于手术前。③手术后食管中段收缩压明显低于手术前。④手术后胃的压力明显低于手术前。⑤手术后食管中段静息压明显低于胃的压力。结论①贲门癌这一疾病并未改变食管运动功能。②手术后近期吻合口处有一高压带,可起到阻止胃腔正压向食管腔传递的压力屏障作用。③手术使食管中段收缩运动功能减弱。④术后反射性引起食管上段收缩增强,从功能上讲似能阻止可能发生的食管咽返流。     

β—淀粉样蛋白及D—半乳糖对大鼠学习记忆和胆碱酯酶活性？…

目的观察β-淀粉样蛋白(β-amyloid,Aβ)、D-半乳糖及两种药物联合使用时对大鼠学习记忆和大脑皮层胆碱酯酶活性的影响.方法D-半乳糖腹腔注射[50mg/(kg@d)],连续6周.在腹腔注射的第4周末,双侧海马内各注射Aβ1μl(含量为4μg).手术后2周,对各组大鼠进行开场行为、Y-型迷宫、被动回避测试及大脑皮层胆碱酯酶活性的测定.结果与对照组相比,D-半乳糖组、Aβ组、D-半乳糖加Aβ组大鼠的自主行为、学习记忆能力和大脑皮层胆碱酯酶的活性明显下降,尤其D-半乳糖加Aβ组明显.结论D-半乳糖、Aβ对大鼠的行为及中枢胆碱能系统均有明显抑制作用,二者联合作用时能产生更强的作用.     

早期TEN和TPN对创伤后免疫和代谢的影响

目的 观察创伤后早期肠内和肠外营养对机体免疫和代谢功能的影响。方法 对复合务患者进行早期肠内或肠外营养支持,观察患者营养支持前后代谢和免疫指标的变化。结果 较之肠外营养,肠内组营养前后前白蛋白变化明显,淋巴细胞增殖率增加显著。而肠外营养组每日氮损失量明显小于肠内营养组。两组间免疫球蛋和Ｔ细胞亚群变化无明显差异 。结论创伤后早期肠内营养支持可促进机体免疫功能恢复,减少并发症发生率。而肠外营养更有利于     

人乳头瘤病毒16型(湖北株)E7基因在体内和体外的表达

构建人乳头瘤病毒１６型（ＨＰＶ１６）Ｅ７湖北株（ＨＢ）基因真核表达质粒,探讨其在哺乳动物体外,体内表达状况,为本地区ＨＰＶ相关肿瘤的基因治疗提供依据。方法采用分子克隆技术,构建ＨＰＶ１６Ｅ７－ＨＢ重组表达质粒,磷酸钙－ＤＮＡ沉淀技术及基因免疫技术将外源目的基因（ＨＰＶ１６Ｅ７－ＨＢ）导入ＮＩＨ３Ｔ３细胞及大、小鼠体内ＰＣＲ,ＲＴ－ＰＣＲ,免疫荧光染色技术对外源目的基因及其产物（ｍＲ－ＮＡ,蛋白质。     

ClO_2毒性研究

ClO_2是一种可以代替液氯的新型消毒剂。本文通过金鱼毒性实验,确定了ClO_2用于饮水消毒的安全浓度;通过小白鼠急性经口毒性实验,确定了ClO_2属实际无毒型水处理剂。     

An outline of spreading of Western mediciine into Yantai in modern age

At the turn of the last century, side by side with the infiltration of religious culture from Western countries, western medicine spread into Yantai. The religious force, headed by American Presbyterian Mission, carried out charitable activities in the process of preaching and laid down the foundation for western medicine rooted in Yantai by establishing schools, education and training followers. They cultivated local physicians by first setting up clinics which had later transformed into hospitals. These are their major measures for building bases for spreading Christianity by foreigners. The Temple Hill Hospital set up by the American physician Oscar F. Hills in Yantai was a successful example of mutual promotion of missionary and medical activities which pushed forward the spreading and development of western medicine in Yantai.     

GIS-based measures of environmental equity: exploring their sensitivity and significance

In order to determine whether principles of environmental justice have been violated, a large number of empirical studies have been carried out to ascertain whether minority and low-income populations are disproportionately exposed to industrial pollution. This study provides a comparative evaluation of two commonly employed proximity measures in GIS-based environmental equity assessment, examining their influence on the results of the analysis, and proposes a methodology for evaluating the significance of these results. 1990 census data on population characteristics and data from the 1995 EPA's toxic release inventory (TRI) for the City of Minneapolis, MN are used. These results also allow a preliminary assessment of environmental equity/inequity in potential exposure to airborne toxic chemicals for racial minorities, poor people and children in Minneapolis. In the third part of the paper we develop and employ a geographic randomization methodology for assessing the significance of these results.