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621.
Immersion ultrasound of testicular pathology 总被引:1,自引:0,他引:1
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Fifty-four patients with severe aplastic anemia were treated with horse anti-human thymocyte globulin (ATG) and androgens. Thirty of these patients also received an infusion of HLA-haploidentical marrow cells. Only those patients having evidence of hematologic recovery within 3 mo after ATG therapy were considered responders to the immunosuppressive regimen. Of 53 patients evaluable for response, 21 had complete or partial responses and 7 had minimal improvement by defined criteria. The remaining patients did not respond or died. Factors correlated with response to therapy included a short duration of aplasia and a high admission granulocyte count. Thirty-six patients (66.7%) are surviving between 18 and 43 mo, and 18 have died. Deaths were due to hemorrhage and/or infection. Short duration of aplasia and high granulocyte counts also correlated with survival, as did younger age. Four patients with complete or partial responses had a recurrence of severe aplasia 6-17 mo after their first course of ATG. Three of these patients were retreated with ATG (and oxymetholone in two cases). All three had second responses to therapy, but two of the three have had second relapses. The fourth patient responded to oxymetholone alone, but died after a second relapse. Mismatched marrow infusion had no effect on the incidence of response or survival. 相似文献
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Estimation of aneuploidy for chromosomes 3, 7, 16, X and Y in spermatozoa from 10 normospermic men using fluorescence in-situ hybridization 总被引:3,自引:0,他引:3
Fluorescence in-situ hybridization (FISH) is a fast and efficient method of
estimating aneuploidy in human spermatozoa. In this study, we have
estimated baseline disomy frequencies in spermatozoa from a group of 10
normospermic men, using stringent scoring criteria. A triple- probe FISH
procedure was used for chromosomes 3, X and Y, while a double-probe FISH
method was used for chromosomes 7 and 16. A total of 101273 spermatozoa
were scored for chromosomes 3, X and Y, resulting in 97.83% haploidy (3X or
3Y), 0.39% disomy (33X, 33Y, 3XX, 3YY or 3XY) and 0.35% diploidy (33XX,
33YY or 33XY). A total of 100760 spermatozoa were scored for chromosomes 7
and 16, giving 98.9% haploidy (716), 0.11% disomy (7716 or 71616) and 0.27%
diploidy (771616). Disomy frequencies for individual chromosomes differed
(chromosome 3, 0.20%; chromosome 7, 0.05%, chromosome 16, 0.06%; X + Y,
0.19%). The frequency of disomy 3 was significantly higher than disomy 7 (P
= 0.019) and disomy 16 (P = 0.022), while the frequency of sex chromosome
disomy was significantly higher than disomy 7 (P = 0.0058) and disomy 16 (P
= 0.0067), but not disomy 3 (P = 0.73). The disomy and diploidy (0.27-
0.35%) estimates obtained for this normospermic population were generally
low and were similar to other recent reports.
相似文献
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Endotoxin-free purified recombinant human erythroid-potentiating activity (EPA) was administered to normal and bled mice. In anemic ICR mice EPA treatment led to a significant increase in the number of reticulocytes in the peripheral blood and erythroid precursors in the spleen. Stimulation of CFU-E and BFU-E in the spleen was also observed in C3H/HEJ mice, which excluded the possibility of endotoxin effect. Unchanged, 51Cr-tagged red cell survival and lack of radioactivity in the stool or urine suggests that the EPA stimulation of erythropoiesis was not due to hemolysis or bleeding. Thus, EPA has an effect on erythropoiesis in anemic mice in vivo. 相似文献
630.
The primary immunodeficiencies are attractive candidates for the development of gene therapy approaches based on the transduction of hematopoietic cells. We have constructed a high-titer recombinant retrovirus for expression of gp91-phox, deficiencies of which cause the X-linked form of chronic granulomatous disease (X-CGD). We have used this vector to transduce human bone marrow, using either unfractionated mononuclear cells or purified CD34+ cells as targets and evaluated several infection protocols. Efficient gene transfer to progenitors and long-term culture-initiating cells (LTC-IC) was obtained for each target population. Importantly for potential clinical application, this could be achieved without the use of exogenous cytokines or polybrene. Progenitors representing each of the lineages detectable in vitro were transduced at equal efficiencies. The vector was shown partially to restore gp91-phox deficiency and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity in transduced cells derived from X- CGD patients. These data demonstrate that it is possible to transduce primitive human hematopoietic cells efficiently and reconstitute NADPH oxidase. 相似文献