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121.
Apostolos E. Kalovidouris Claire A. Horn Zoya Plotkin 《Arthritis \u0026amp; Rheumatology》1994,37(6):907-914
Objective. To investigate the effect of interferon-γ (IFNγ) on the adhesive interactions between human peripheral blood T cells and human skeletal muscle cells at various stages of muscle cell differentiation and maturation in vitro. Methods. Human muscle cell cultures were established from normal muscle biopsy material, using the explant technique. T cells were studied for their capacity to adhere to IFNγ-treated and untreated myoblasts and myotubes. The role of intercellular adhesion molecule type 1 (ICAM-1) in cell adhesion to muscle cells was examined in blocking studies, by enzyme-linked immuno-sorbent assay (ELISA), and by immunohistochemical staining using monoclonal antibodies (MAb). Results. Treatment of muscle cells (myoblasts and myotubes) with IFNγ resulted in a significant dose-dependent increase in the number of adherent T cells. Adhesion of T cells to muscle cells was significantly inhibited by MAb to ICAM-1 and to lymphocyte function–associated antigen type 1, but not by MAb to HLA–DR. There was no difference in the level of T cell adhesion to IFNγ-treated allogeneic versus autologous muscle cells. By ELISA and immunohistochemical analysis, ICAM-1 expression on the surface of cultured human muscle cells was either absent or barely detectable, but was strongly induced by treatment of muscle cells with IFNγ. Conclusion. Induction of cell adhesion molecules on muscle cells by IFNγ may be an important mechanism for the localization of T cells in the affected muscles of patients with autoimmune myositis. 相似文献
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Eva Freier Cora Stefanie Weber Ulrike Nowottne Christiane Horn Katrin Bartels Sabrina Meyer York Hildebrandt Tim Luetkens Yanran Cao Caroline Pabst Julia Muzzulini Benjamin Schnee Monika Christine Brunner-Weinzierl Maurizio Marangolo Carsten Bokemeyer Hans-Christian Deter Djordje Atanackovic 《Psychoneuroendocrinology》2010
125.
Suhal S Mahid Daniel W Colliver Nigel PS Crawford Benjamin D Martini Mark A Doll David W Hein Gary A Cobbs Robert E Petras Susan Galandiuk 《BMC medical genetics》2007,8(1):28
Background
N-acetyltransferase 1 (NAT1) and 2 (NAT2) are polymorphic isoenzymes responsible for the metabolism of numerous drugs and carcinogens. Acetylation catalyzed by NAT1 and NAT2 are important in metabolic activation of arylamines to electrophilic intermediates that initiate carcinogenesis. Inflammatory bowel diseases (IBD) consist of Crohn's disease (CD) and ulcerative colitis (UC), both are associated with increased colorectal cancer (CRC) risk. We hypothesized that NAT1 and/or NAT2 polymorphisms contribute to the increased cancer evident in IBD. 相似文献126.
Origin of trisomy 12 in B-cell chronic lymphocytic leukemia 总被引:1,自引:0,他引:1
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Lucas A. Horn Paul L. Chariou Sofia R. Gameiro Haiyan Qin Masafumi Iida Kristen Fousek Thomas J. Meyer Margaret Cam Dallas Flies Solomon Langermann Jeffrey Schlom Claudia Palena 《The Journal of clinical investigation》2022,132(8)
Collagens in the extracellular matrix (ECM) provide a physical barrier to tumor immune infiltration, while also acting as a ligand for immune inhibitory receptors. Transforming growth factor-β (TGF-β) is a key contributor to shaping the ECM by stimulating the production and remodeling of collagens. TGF-β activation signatures and collagen-rich environments have both been associated with T cell exclusion and lack of responses to immunotherapy. Here, we describe the effect of targeting collagens that signal through the inhibitory leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) in combination with blockade of TGF-β and programmed cell death ligand 1 (PD-L1). This approach remodeled the tumor collagenous matrix, enhanced tumor infiltration and activation of CD8+ T cells, and repolarized suppressive macrophage populations, resulting in high cure rates and long-term tumor-specific protection across murine models of colon and mammary carcinoma. The results highlight the advantage of direct targeting of ECM components in combination with immune checkpoint blockade therapy. 相似文献
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Dupuy AJ Clark K Carlson CM Fritz S Davidson AE Markley KM Finley K Fletcher CF Ekker SC Hackett PB Horn S Largaespada DA 《Proceedings of the National Academy of Sciences of the United States of America》2002,99(7):4495-4499
Transposons have been used in invertebrates for transgenesis and insertional mutagens in genetic screens. We tested a functional transposon called Sleeping Beauty in the one-cell mouse embryo. In this report, we describe experiments in which transposon vectors were injected into one-cell mouse embryos with mRNA expressing the SB10 transposase enzyme. Molecular evidence of transposition was obtained by cloning of insertion sites from multiple transgenic mice produced by SB10 mRNA/transposon coinjection. We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse. 相似文献