A study on clinical effect of Arbidol combined with adjuvant therapy on COVID-19

This study aims to explore the clinical effect of Arbidol (ARB) combined with adjuvant therapy on patients with coronavirus disease 2019 (COVID-19). The study included 62 patients with COVID-19 admitted to the First Hospital of Jiaxing from January to March 2020, and all patients were divided into the test group and the control group according to whether they received ARB during hospitalization. Various indexes in the two groups before and after treatment were observed and recorded, including fever, cough, hypodynamia, nasal obstruction, nasal discharge, diarrhea, C-reactive protein (CRP), procalcitonin (PCT), blood routine indexes, blood biochemical indexes, time to achieve negative virus nucleic acid, and so on. The fever and cough in the test group were relieved markedly faster than those in the control group (P < .05); there was no obvious difference between the two groups concerning the percentage of patients with abnormal CRP, PCT, blood routine indexes, aspartate aminotransferase, and alanine aminotransferase (P > .05); the time for two consecutive negative nucleic acid tests in the test group were shorter than that in the control group; the hospitalization period of the patients in the test group and control group were (16.5 ± 7.14) days and (18.55 ± 7.52) days, respectively. ARB combined with adjuvant therapy might be able to relieve the fever of COVID-19 sufferers faster and accelerate the cure time to some degree, hence it's recommended for further research clinically.     

Quercetin alleviates neonatal hypoxic-ischemic brain injury by inhibiting microglia-derived oxidative stress and TLR4-mediated inflammation

Inflammation Research - Microglia stimulated by oxygen glucose deprivation (OGD) were treated with quercetin to investigate the effect on oxidative stress and the inflammatory response and to...     

数字化三维重建技术在隐神经营养血管皮瓣血供的可视化研究及临床应用

目的探讨数字化三维重建技术在隐神经营养血管皮瓣血供可视化研究的可行性及其临床应用效果。 方法新鲜捐献成人尸体1具,采用改良的明胶-氧化铅灌注方法进行灌注,灌注前和灌注后均用64排螺旋CT进行扫描,利用Mimics 10.01软件,获得血管、肌肉、骨骼以及皮肤的三维解剖结构图像,并对血管进一步分割,构建出单支血管及其分支,并对所构建的三维图像进行单独显示与组合显示,以设计切取隐神经营养血管皮瓣。在上述基础上,选择于2015年12月至2018年1月住院的小腿下段及足踝部皮肤软组织缺损需行隐神经营养血管皮瓣修复手术患者40例,随机分成A、B两组,每组20例,A组术前进行数字化重建,B组术前不予数字化重建。比较2组术后隐神经营养血管皮瓣存活率,手术所需时间,精确性以及皮瓣旋转点到内踝顶点距离。 结果灌注前数据可轻易重建出骨、肌肉、筋膜与皮肤,灌注后的数据可构建出血管,组合显示后可得到血管在某个局部的具体分布状况,为隐神经营养血管皮瓣的临床个性化设计与基础研究提供准确的血管解剖学基础。A组隐神经营养血管皮瓣经数字化重建后,可显示皮瓣主要血供,并能够清晰显示皮肤、肌肉与血管三维解剖关系;应用软件自带测量工具对血管、皮瓣进行测量,可以准确定位指导手术操作。术中探查血供与重建图像相符,体表定位准确。A组20例皮瓣均成活良好,伤口一期愈合,皮瓣质地良好,局部无瘢痕挛缩;B组20例皮瓣4例患者于术后出现皮瓣远端部分坏死,经二期清创换药和植皮术后愈合。术后随访3～12个月,A组皮瓣质地良好,外形满意,B组有6例皮瓣远端出现质硬瘢痕和轻度挛缩,行走时疼痛,后经手术松解植皮后好转。A组皮瓣成活率(100%)明显高于B组(70%),差异有统计学意义(P<0.05);A组手术所需时间[(114.05±10.36) min]明显短于B组[(123.20±11.91) min],差异有统计学意义(P<0.01);A组皮瓣切取精确性(0.17±0.03)明显高于B组(0.23±0.02),差异有统计学意义(P<0.01);A组皮瓣蒂部旋转点到内踝顶点距离[(4.14±0.38) cm]明显少于B组[(5.96±0.81) cm],差异有统计学意义(P<0.01)。 结论数字化三维重建技术为隐神经营养血管皮瓣临床手术的个性化设计提供了一个很好的辅助手段,临床应用修复小腿远端及足踝部软组织缺损效果满意,值得临床推广应用。     

Perfectionistic traits and self‐presentation are associated with negative attitudes and concerns about seeking professional psychological help

Numerous factors influence whether an individual is able and willing to seek professional help for psychological difficulties. One of these may be perfectionism, a multidimensional personality construct that has been linked to poor psychological adjustment. The current study investigated whether perfectionism traits and self‐presentational facets were associated with negative help‐seeking attitudes and concerns about psychotherapy. Samples of university (N = 299) and community (N = 77) men and women completed the Multidimensional Perfectionism Scale, Perfectionistic Self‐Presentation Scale, Attitudes Towards Seeking Professional Help Scale, and Thoughts About Psychotherapy Survey. Various components of perfectionism were associated with both negative help‐seeking attitudes and concerns about psychotherapy. The findings suggest that perfectionistic behaviour may be an important dispositional factor that interferes with seeking and obtaining help for psychological difficulties. Theoretical and clinical implications are discussed.     

A protective polymorphism in MMP16, improved blood gas levels,and chronic obstructive pulmonary diseases: Family and two population‐based studies

The aberrant expression of matrix metalloproteinases (MMPs) is known to contribute to the pathogenesis of airway remodeling and alveolar disruption in chronic obstructive pulmonary disease (COPD). In the discovery stage, 11 COPD from five families were subjected to whole‐genome sequencing, and 21 common polymorphisms in MMPs and TIMPs were identified. These polymorphisms were genotyped in two subsequent verification studies. Of these polymorphisms, c.2392G>A (rs2664370T>C) and c.4158C>A (rs2664369T>G) in MMP16 remained significantly different. Functionally, we found that MMP16 expression was significantly increased in peripheral blood monocytes (PBMCs) from COPD and in cigarette smoke extract‐treated 16HBE cells compared with controls. This was also shown by bioinformatics analysis. COPD carrying rs2664370CC showed decreased levels of MMP16 in the plasma and in PBMCs compared with those carrying CT and TT. Treatment with hsa‐miR‐576‐5p mimics led to a greater reduction in luciferase reporter activity in cells transfected with rs2664370CC. Moreover, blood levels of base excess, PCO₂, and PO₂ in COPD with rs2664370CC were significantly lower than those with rs2664370CT+TT. Taken together, these results demonstrate that the rs2664370T>C polymorphism in MMP16 protects against the risk of COPD, likely by favoring interaction with hsa‐miR‐576‐5p, leading to reduced MMP16 expression and improved blood gas levels.     

Identification of pathogenic variants of ERLEC1 in individuals with Class III malocclusion by exome sequencing

Class III malocclusion is a common dentofacial deformity. The underlying genetic alteration is largely unclear. In this study, we sought to determine the genetic etiology for Class III malocclusion. A four‐generation pedigree of Class III malocclusion was recruited for exome sequencing analyses. The likely causative gene was verified via Sanger sequencing in an additional 90 unrelated sporadic Class III malocclusion patients. We identified a rare heterozygous variant in endoplasmic reticulum lectin 1 (ERLEC1; NM_015701.4(ERLEC1_v001):c.1237C>T, p.(His413Tyr), designated as ERLEC1‐m in this article) that cosegregated with the deformity in pedigree members and three additional rare missense heterozygous variants (c.419C>G, p.(Thr140Ser), c.419C>T, p.(Thr140Ile), and c.1448A>G, p.(Asn483Ser)) in 3 of 90 unrelated sporadic subjects. Our results showed that ERLEC1 is highly expressed in mouse jaw osteoblasts and inhibits osteoblast proliferation. ERLEC1‐m significantly enhanced this inhibitory effect of osteoblast proliferation. Our results also showed that the proper level of ERLEC1 expression is crucial for proper osteogenic differentiation. The ERLEC1 variant identified in this study is likely a causal mutation of Class III malocclusion. Our study reveals the genetic basis of Class III malocclusion and provides insights into the novel target for clinical management of Class III malocclusion, in addition to orthodontic treatment and orthodontic surgery.     

术前评分系统在高龄老年患者髋部骨折围手术期风险评估中的应用探讨

目的 探讨术前评分系统在高龄老年髋部骨折围手术期风险评估中的应用价值,并评估短期随访效果。方法2017年3月至2019年3月本科对94例75岁以上的高龄老年髋部骨折患者行微创手术治疗,术前将患者的生理指标进行赋值评分,结合手术风险的赋值评分,二者之和作为整个手术风险分值,以20分为低风险组,21～25分为中风险组,26～30分为高风险组,30分为极高风险组,将其分组后比较各组之间术后并发症的发生率和术后1个月内的死亡率。结果 根据术前整个手术风险分值的高低,将患者划分到不同的风险组,分值越高所在的风险组级别越高,术后并发症的发生率低风险组与高、极高风险组之间、中风险组和极高风险组之间比较,差异有统计学意义(0.05)。本组94例纳入研究的病例中术后1个月内除了极高风险组死亡1例外,其余风险组未发生死亡病例,统计学比较差异无统计学意义(0.05)。结论 术前评分系统可以客观地预估高龄老年髋部骨折患者的手术风险和预后,对临床有指导意义。