食管癌高发及低发区患者DNA修复功能的研究

本文对食管癌高、低发区食管癌患者和正常人,经MNNG诱导的DNA损伤修复功能进行了研究。实验采用外周血淋巴细胞培养的方法。每组又分为对照组及MNNG诱导组。样品以~3H-TdR标记后经液闪仪计数法进行检测。结果发现,低发区正常人经MNNG诱导的DNA损伤修复功能明显高于对照组,两个地区食管癌患者修复能力均低于正常人,与地区无关。     

The correlation of ureaplasma urealyticum infection with infertility

Summary. A prevalence study of Ureaplasma urealyticum (UU) infection of the male genital tract was carried out in Shanghai between March 1992 and June 1995. Significantly higher frequency of UU infection was found among infertile males (549/1416) as compared to fertile controls (34/375). Examination of 8 specimens each from infertile men and fertile subjects by electron microscopy, immunogold and immunofluorescence techniques, demonstrated adhesion of Ureaplasma urealyticum to the membrane of spermatozoa and exfoliated germ cells. In addition, gold particles on Ureaplasma urealyticum were found to be adhered to the sperm surface in 4 of the 8 samples. Strong specific anti-UU fluorescence was detected in 6 of 8 samples, mainly on the midpieces and post-acrosomal regions of the spermatozoa.
To further study the effects of Ureaplasma urealyticum on fertility, 47 male Sprague-Dawley (SD) rats were infected artificially with Ureaplasma urealyticum serotype 8 (T960). Morphological changes in the seminiferous tubules were observed 3–5 weeks after inoculation in the sacrificed animals. Dramatic impairment of spermatogenesis of both testes was found in 11 rats. Mating experiment confirmed infertility in 12 of 40 rats. Offsprings of the infected rats were significantly smaller than those of controls in terms of prenatal weights and birthweights.     

Histopathologic findings in a case of recurrent parotitis in adulthood

Most cases with recurrent parotitis in childhood (RPC) can be cured before puberty.^1–3 A few of them, however, may continue to adulthood.^1–4 To distinguish this condition from other types of chronic parotitis in adults, such as chronic obstructive parotitis,⁵ we suggest that the diagnosis of recurrent parotitis in adults (RPA) be reserved for the cases derived from RPC.⁶ The pathophysiology and development of RPA is still not well known. To our knowledge, there are few reports of RPA in the literature, especially concerning its histopathology. This report describes a cases of RPA studied with sialography and light and electron microscopy.     

葡萄糖酸锌糖浆的制备与含量测定方法

本文报告了葡萄糖酸锌糖浆的制法及用二甲橙指示剂法测定含量的方法。结果表明制法简便,含量测定方法准确。     

深低温冷冻保存家兔性腺器官的研究

自１９９２年７月开始行深低温（－１９６℃）冷冻保存兔性腺研究。冷冻睾丸３６个，１５天后解冻，完好率９１．７％，其中２０个行器官移植，睾丸接通血管均即时建立血循环。４只兔的冷冻睾丸自体移植后，每次采精７４０．６±１８９０．４万个。２只兔的冷冻睾丸异体移植后，每次采精６３．４±３７．７万个。冷冻兔卵巢１４个，１５天后解冻，完好率９２．９％，其中８个行器官移植，卵巢接通血管均即时建立血循环，术后雌二醇水平为２．７２～１２７．３ｐｍｏｌ／Ｌ。冷冻睾丸及卵巢移植一段时间后病理切片证实睾丸及卵巢组织存活。     

大,巨型垂体腺瘤的手术入路选择

自１９９０年以来收治直径在２－５．６ｃｍ的大、巨型垂体腺瘤３３例。依肿瘤生长形态与扩展范围将其简略分为三种类型。Ａ型：瘤体位于鞍内或侵入蝶窦；Ｂ型；瘤体呈椭圆形或哑铃向鞍上扩展，三脑室明显移位抬高；Ｃ型：瘤体巨大侵入三脑室阻塞室间孔或明显的鞍周扩展。     

刺激强度对黑质DA能神经元伤害性反应的影响

本研究用细胞外记录方法研究大鼠黑质多巴胺能神经元伤害性反应的特点。共记录了194个多巴胺能神经元。其中,大多数神经元(78％)可被尾部强电刺激(15mA,1.0ms)所抑制,15％被兴奋。兴奋和抑制反应均依赖于刺激强度。当刺激强度变化于0～20mA时,伤害性反应强度与刺激强度的对数显著相关。来自不同部位的刺激可会聚于同一神经元。反应潜伏期和阈值提示Aδ纤维参与伤害性信息传入黑质的过程。本文还讨论了多巴胺能神经元系统在痛觉机制中的作用。     

耳廓复合组织瓣连续法修复鼻翼缺损

目的观察耳廓复合游离组织瓣连续法修复鼻翼缺损的疗效，以探讨鼻翼缺损的有效修复手段。方法耳廓复合游离组织瓣2次连续法修复鼻翼缺损7例，移植物面积0．5cm×0．7cm～0．8cm×1．2cm。结果7例耳廓复合游离组织瓣移植后全部成活。术后随访6～18个月，移植物无明显回缩，鼻外形满意。结论耳廓复合游离组织瓣连续移植修复鼻翼缺损，适合较严重鼻翼缺损，手术成功率高，术后鼻外形满意，双侧分次取材，耳廓外形双侧对称无畸形。