温胆汤改良方对Aβ(25-35)诱导AD细胞模型bcl-2、bax蛋白表达的影响

目的:温胆汤改良方含药脑脊液及含药血清对AD细胞模型凋亡及凋亡相关蛋白bcl- 2、bax表达的影响。方法:温胆汤改良方含药脑脊液和含药血清分别作用于Aβ25-35诱导NG108-15 细胞建立的AD细胞模型,镜下观察细胞生长状况,流式细胞术检测细胞凋亡,免疫组化检测bcl- 2、bax蛋白的表达。结果:与模型组比较,含药脑脊液组和含药血清组细胞凋亡率降低（p<0．05）, bel-2表达无显著性差异（p>0．05）,bax蛋白表达减弱（p<0．01）,bcl-2／bax比值升高（p<0．01）。结论:温胆汤改良方能有效地抑制AD细胞模型凋亡,其作用机理可能与其干预凋亡相关基因bax的表达,升高bcl-2／bax比值有关。     

温胆汤改良方对Aβ25-35诱导AD细胞模型bcl-2、bax蛋白表达的影响

目的：温胆汤改良方含药脑脊液及含药血清对AD细胞模型凋亡及凋亡．相关蛋白bcl-2、bax表达的影响。方法：温胆汤改良方含药脑脊液和含药血清分别作用于Aβ25-35诱导NG108-15细胞建立的AD细胞模型，镜下观察细胞生长状况，流式细胞术检测细胞凋亡，免疫组化检测bcl-2、bax蛋白的表达。结果：与模型组比较，含药脑脊液组和含药血清组细胞凋亡率降低（p〈0．05），bcl-2表达无显著性差异（p〉0、05），bax蛋白表达减弱（p〈0．01），bcl-2／bax比值升高（p〈0．01）。结论：温胆汤改艮方能有效地抑制AD细胞模型凋亡，其作用机理可能与其干预凋亡相关基因bax的表达，升高bcl-2／bax比值有关。     

灌肠方保留灌肠治疗溃疡性结肠炎34例

为研究中药对溃疡性结肠炎(UC)的疗效,应用中药灌肠方保留灌肠为主,治疗溃疡性结肠炎(UC)34例,疗程4周。近期总有效率97．06％,无肠穿孔、大出血及中毒性巨结肠等并发症。随访18例,2年内未发现癌变,18例患者半年内复发率5．56％,1年内复发率10．72％,2年内复发率17．86％。提示中药具有抑菌、收敛、止血、镇痛、保护黏膜、促进血液循环、减轻水肿和渗出等作用,直接局部给药,治疗病变部位在乙状结肠以下的UC,具有较好的近期疗效。     

护理本科生实习后期存在的问题与对策

随着社会进步和科学发展,新的医学、护理模式的形成,对护士的素质、知识、能力提出了更高的要求。发展高等护理教育,提高护理队伍学历层次成为护理教育的发展方向[1]。将护理本科生造就成为具有临床护理、教学、科研、管理能力[2]的高级护理人才正是顺应了这一形势。临床实习是     

补阳还五汤对心肌梗死后患者心脏重塑及心功能的作用研究

摘要 目的:研究补阳还五汤对心肌梗死后患者心脏重塑及心功能改善的作用。方法:选择130例心肌梗死患者临床资料,将其随机分为对照组和观察组,每组65例患者。对照组采用常规西药治疗,观察组在对照组治疗的基础上加服补阳还五汤,治疗5个疗程（1个月为1个疗程） 后观察对比两组患者心脏重塑及心功能改善情况。结果:观察组总有效率92.30%明显高于对照组76.92% （P＜0.05）;治疗后两组左室舒张末内径(LVDd)、左室收缩期内径(LVDs)、左室后壁厚度(LVPWT)、左室心肌重量(LVM)均明显改善,与治疗前对比（P＜0.05）,且观察组改善明显优于对照组（P＜0.05）;治疗后两组左室收缩末期容积(LVESV)、左心室舒张末期容积(LVEDV)、每搏输出量(SV)、心搏量(CO)、射血分数(EF)、二尖瓣E波峰值流速/A波峰值流速(E/A)均明显改善,与治疗前对比（P＜0.05）,且观察组改善明显优于对照组（P＜0.05）;治疗后两组中医临床症状评分明显降低,与治疗前对比（P＜0.05）,且观察组明显低于对照组（P＜0.05）;观察组并发症发生率明显低于对照组,差异有统计学意义（P＜0.05）.结论:补阳还五汤对心肌梗死后患者心脏重塑及心功能改善具有显著的作用,改善心肌梗死患者预后,具有临床应用的重要意义。     

Monocyte to high‐density lipoprotein cholesterol ratio as an independent risk factor for papillary thyroid carcinoma

BackgroundPapillary thyroid carcinoma (PTC) is considered to be an inflammatory disease. This study aimed to investigate the association of monocyte to high‐density lipoprotein cholesterol ratio (MHR) with PTC.MethodsClinical parameters from 300 patients with PTC and 552 patients with benign thyroid nodule were compared. Serum renal function and liver enzymes, fasting plasma glucose, lipid profile, and blood cell count were measured.ResultsPatients with PTC had a higher MONO (p < 0.001) and MHR (p < 0.001). There was a step‐wise increase in the prevalence of PTC (p = 0.003) with the tertile of MHR. Logistic regression analysis revealed that MHR could be considered an independent risk factor (p < 0.001) in the case‐control study and the cohort study. Pearson correlation analysis and simple linear regression analysis indicated that MHR was positively associated with neutrophil (NEU) and lymphocyte (LYM) count as well as neutrophil‐to‐lymphocyte ratio (NLR). Area under the curve (AUC) was 0.711. The optimal cutoff of MHR was 0.33 × 10⁹/mmol.ConclusionThis study identifies novel evidence that patients with PTC have a higher MHR. MHR is an independent risk factor for PTC. These findings support the application of MHR to predict, diagnose, and evaluate the occurrence of PTC.     

Green tea polyphenols inhibit colorectal aberrant crypt foci (ACF) formation and prevent oncogenic changes in dysplastic ACF in azoxymethane-treated F344 rats

Green tea and its constituents have shown cancer-preventive activities in many animal models. In order to prepare for a human trial on the inhibition of colon carcinogenesis, we conducted a study with green tea polyphenols as the preventive agent in an azoxymethane (AOM)-induced rat colon cancer model using aberrant crypt foci (ACF) as an end point. F344 rats were given two weekly injections of AOM (15 mg/kg), and then fed a 20% high-fat diet with or without 0.12 or 0.24% Polyphenon E (PPE, a standardized green tea preparation consisting 65% of (-)-epigallocatechin-3-gallate and 22% of other catechins) for 8 weeks. Colorectal ACF were analyzed under a microscope after methylene blue staining. Dietary PPE administration was found to significantly and dose dependently decrease the total number of ACF per rat and the total number of aberrant crypt per rat. Moreover, treatment with 0.24% PPE also significantly decreased the percentage of large ACF (four or more crypts) and the percentage of ACF with high-grade dysplasia in total ACF. The high-grade dysplastic ACF from 0.24% PPE-treated group had increased apoptosis and decreased nuclear expression levels of beta-catenin and cyclin D1. Retinoid X receptor (RXR)alpha expression was reduced in high-grade dysplastic ACF, adenoma and adenocarcinoma during AOM-induced colon carcinogenesis, and the PPE treatment partially prevented the loss of RXRalpha expression in high-grade dysplastic ACF. Taken together, our results strongly suggest the colon cancer-preventive activity of PPE and identified possible molecular markers for future colon cancer prevention studies.     

手法小切口白内障青光眼联合手术48例的临床观察

目的探讨手法小切口白内障摘出联合小梁切除术的临床疗效。方法急性闭角型青光眼合并白内障48例(52眼)在手法小切口白内障摘出术的巩膜隧道切口内作小梁切除术。观察术后视力、眼压及并发症。结果术后7天最佳矫正1.0有2眼,0.6-0.8有13眼,0.4-0.5有25眼,0.2-0.3有7眼,<0.1有5眼。术后视力平均提高3-4行,手术前后最佳矫正视力差异有统计学意义(<0.001)。术后眼压(12.20±2.45)mmHg。术后1周眼压9～18mmHg,平均降低8.25mmHg;3个月后术后眼压21mmHg以下49眼,眼压控制率为92.48%。术后眼压低于术前,差异有统计学意义(t=21.56,=0.000)。术后发生角膜内皮中度水肿3眼,前房积血2眼,后发性白内障2眼。结论手法小切口白内障摘出联合小梁切除术具有操作简单、损伤小、视力恢复快、降压效果好的优点,可有效治疗青光眼合并白内障。     

小剂量长时间口服罗红霉素治疗常年性变应性鼻炎的临床研究

目的探讨罗红霉素(roxithromycin,RXM)是否对常年性变应性鼻炎(perennial allergic rhinitis,PAR)有治疗作用及其可能的作用机制,为临床治疗常年性变应性鼻炎探索一种新途径.方法常年性变应性鼻炎患者111例,随机分为常规免疫治疗组(对照组)51例,RXM并常规免疫治疗组(试验组)60例.结果 (1)治疗12周后,试验组总有效率为80%;对照组总有效率为19.61%,差异非常显著(P＜0.01).(2)治疗后试验组鼻分泌物嗜酸性粒细胞(EOS)计数较对照组低,差异有显著性(P＜0.05),较治疗前降低(P＜0.01).(3)治疗后,试验组血清细胞因子IL-5、IL-8浓度较对照组低,差异有显著性(P＜0.05,P＜0.01);IL-5、IL-8浓度较治疗前降低明显(P均＜0.01).结论小剂量长时间口服RXM治疗PAR有效,可能通过减少机体细胞因子IL-5、IL-8,抑制EOS在鼻黏膜的聚集而发挥其抗炎、调节免疫的作用.     

头环牵引外固定架在治疗颈椎外伤中的应用

目的：探讨头环牵引外固定架治疗颈椎外伤的临床疗效。方法：123例颈椎外伤性骨折脱位患者，随机采用两种方法治疗，65例行头环牵引外固定（头环组）；58例行颅骨牵引（颅骨组）。结果：头环组与颅骨组对上颈椎骨折脱位的复位作用差别无统计学意义，对下颈椎骨折脱位的复位作用头环组要明显好于颅骨组。治疗后离床时间头环组要短于颅骨组。结论：头环牵引外固定架可改善复位功能，对颈椎的牵引固定作用较颅骨牵引更为坚强稳定，使用安全方便。