脑清喷鼻微乳治疗急性缺血性脑卒中100例随机对照临床研究

目的:观察脑清喷鼻微乳治疗急性缺血性脑卒中的临床疗效。方法:共100例急性缺血性脑卒中患者纳入研究,采用随机数字表法分为脑清喷鼻微乳组(治疗组)与空白安慰剂组(对照组)各50例。两组患者均采用常规基础治疗,治疗组予脑清喷鼻微乳喷鼻,2喷/次,3次/d;对照组予不含药物成分的微乳,2喷/次,3次/d。以美国国立卫生研究院卒中量表(NIHSS)、Fugl-Meyer运动功能评定量表、生活能力状态表、中风症状分级量化表及血液流变学检查评估两组患者治疗治疗前与治疗后7天、14天的愈显率及有效率。结果:治疗组总有效率93.33%,愈显率60.00%,其愈显率明显高于对照组的42.00%(P<0.05)。在NIHSS评分、Fugl-Meyer运动功能评定量表、日常生活能力量表积分、中医症候积分及血液流变学改善方面均明显优于对照组(P<0.05)。结论:脑清喷鼻微乳经鼻给药治疗急性缺血性脑卒中疗效肯定,可改善患者的神经功能状况,中医症状及血流变指标,提高其日常生活能力,为临床治疗提供依据。     

脐血干细胞移植对大鼠脊髓损伤后轴突再生的影响

目的:探讨脐血千细胞移植对大鼠脊髓损伤后轴突再生的影响.方法:收集脐带血,分离提取,制备成合适浓度的CD34阳性细胞.Allen重物坠击法制作SD大鼠急性脊髓损伤动物模型30只,随机分成2组.A组为损伤对照组,B组为细胞移植组,1周后再次手术,细胞移植组将体外培养的脐血干细胞用微量注射器分别注入于脊髓损伤区域头侧和尾侧各10x105个细胞,损伤对照组予以同样体积的PBS,于移植后1周、2周、6周时分别取材,进行组织学检查及免疫组织化学检杏以评估损伤区域脊髓形态学变化,并于移植后1周、2周、4周、6周采用BBB评分及斜板实验,以观察大鼠后肢运动神经功能恢复情况.结果:与对照组比较,细胞移植组大鼠脊髓损伤区域空腔面积显著减小,生长相关蛋白(GAP-43)和神经丝蛋白(NF200)表达显著增加,并且后肢运动功能均有较明显的恢复.结论:脐血干细胞移植可以促进脊髓损伤大鼠轴突再生和后肢运动功能恢复.     

Ablation of Wntless in endosteal niches impairs lymphopoiesis rather than HSCs maintenance

Osteoblasts and perivascular stromal cells constitute essential niches for HSC self‐renewal and maintenance in the bone marrow. Wnt signaling is important to maintain HSC integrity. However, the paracrine role of Wnt proteins in osteoblasts‐supported HSC maintenance and differentiation remains unclear. Here, we investigated hematopoiesis in mice with Wntless (Wls) deficiency in osteoblasts or Nestin‐positive mesenchymal progenitor cells, which presumptively block Wnt secretion in osteoblasts. We detected defective B‐cell lymphopoiesis and abnormal T‐cell infiltration in the bone marrow of Wls mutant mice. Notably, no impact on HSC frequency and repopulation in the bone marrow was observed with the loss of osteoblastic Wls. Our findings revealed a supportive role of Wnts in osteoblasts‐regulated B‐cell lymphopoiesis. They also suggest a preferential niche role of osteoblastic Wnts for lymphoid cells rather than HSCs, providing new clues for the molecular nature of distinct niches occupied by different hematopoietic cells.     

Novel polymeric acidic ionic liquids as green catalysts for the preparation of polyoxymethylene dimethyl ethers from the acetalation of methylal with trioxane

A series of micro–mesoporous polymeric acidic ionic liquids (PAILs) have been successfully synthesized and subsequently characterized using Fourier transform-infrared spectroscopy, N₂ adsorption–desorption isotherms, scanning electron microscopy and thermogravimetry. Furthermore, the catalytic performance of the synthesized PAILs was investigated for the acetalation of methylal (DMM₁) with 1,3,5-trioxane (TOX), micro–mesoporous PAILs copolymerized by divinylbenzene with cations and anions exhibited moderate to excellent catalytic activities for the acetalation. In particular, VIMBs–AMPs–DVB, with higher specific surface area (25.51 m² g⁻¹) and total pore volume (0.15 cm³ g⁻¹) displayed an elevated conversion of formaldehyde (82.2%) and selectivity for polyoxymethylene dimethyl ethers (CH₃O(CH₂O)_nCH₃; PODE_n or DMM_n) n = 3–8 (52.6%) at 130 °C, 3.0 MPa for 8 h. Moreover, the influence of various reaction parameters was investigated by employing VIMBs–AMPs–DVB as the catalyst and it demonstrated high thermal stability and easy recovery.

Polyoxymethylene dimethyl ethers were successfully synthesized from acetalation under the catalysis of novel polymeric acidic ionic liquids (PAILs). PAILs copolymerized by divinylbenzene with ILs displayed exceptional catalytic efficiencies.     

内镜注射治疗上消化道出血

经内镜注射复方消痔灵、无水酒精、高渗盐水。肾上腺素－利多卡因等治疗上消化道出血２６４例，即刻止血率９９．６２％，仅１例再出血转外科手术止血，无并发症。上消化道出血经内镜局部注射止血，操作简便，药源充足，价格低廉，值得推广应用。     

内镜高频电刀切除食管癌术后吻合口狭窄

用自制针式高频电刀于内镜下切除食管癌术后吻合口疤痕狭窄组织。共进行８９例、２６２次。其中，中上段狭窄５２例（５８．４２％），中下段狭窄３７例（４１．５８％）。吻合口＜２．０ｍｍ呈闭合状４０例，＜３．０ｍｍ１７例，＞３．０ｍｍ１６例，有１６例被食物团嵌顿。切除疤痕后１次即通畅者６２例，作２次、３次通畅者各１１例，作８１次、４３次、１２次、５次、４次通畅者各１例（前２例为膜蹼样疤痕增生狭窄，每１－３月需重复治疗１次）。本项治疗技术疗效可靠，安全性好，值得推广。     

经尿道等离子双极电切治疗高龄高危前列腺增生症

目的总结经尿道等离子双极电切治疗高龄高危前列腺增生症患者的经验和观察其临床疗效。方法回顾分析我科于2015年1月~2017年6月采用经尿道等离子双极电切术,治疗60例高龄高危前列腺增生症的临床资料,记录术前患者的PSA、IPSS、QOL,统计手术时间、出血量、并发症发生率、术后住院时间等资料。结果本组60例患者均顺利完成,平均手术时间103±32.3 min,平均出血量62.6±34.4 mL,无输血,术后平均住院时间平均7.4±1.1 d。术后3月Qmax 19.8~26.3 mL/s,平均23.2±1.7 mL/s;IPSS 6~17分,平均11.6±2.5分;QOL 0~3分,平均1.26±0.78分,与术前相比差异有统计学意义,t值分别为31.1、29.5、36.6,所有P0.001。术后5例出现暂时性尿失禁,1例发生术后尿道狭窄,经对症处理情况改善后出院。平均随访15.68±7.32月,均未出现尿潴留,排尿畅。结论经尿道等离子双极电切治疗高龄高危前列腺增生症患者是安全的、有效的。     

Load Assessment of the Static Posture and Weight Holding inTerms of Pulmonary Ventilation and Heart Rate

Therewerefewstudiesonwhetherenergymetabolismorheartrate(HR)canbeusedasanindex'toreflectstaticpostureandweightholdingloadandtheresultswerecontradictroy['-'J.Weobservedchangesinpulmonaryventilation(VE)andHRundertheconditionofstaticposturesandweightholdingsin35studentsinordertoobtainappropriateparameters.1SUBJECTSANDMETHODS1'1SubjectsThirty--fivestudents(10femalesand25males)wasincludedinthestudy.Themeanage,bodyweightandheightofthefemalesubjectswere22.Ito.7years,49.8f3.7kg,158.3t3.3cman…     

胸腰椎爆裂骨折后路手术复位效果与手术时间的关系

目的：分析胸腰椎爆裂骨折后路手术间接复位的效果与手术时间的关系。方法：将35例符合研究标准的患者按受伤到手术时间的不同分为2组，受伤7d内手术的为早期手术组共20例，受伤7-14d手术的为晚期手术组共15例。以Cobb角和椎管占位比例作为间接复位效果评价标准。结果：2组患者术前Cobb角及椎管占位比例差异均无统计学意义（P〉0．05），但术后Cobb角及椎管占位比例早期组均低于晚期组，差异有统计学意义（P〈0．05）。结论：胸腰椎爆裂骨折后路手术间接复位的效果与手术时间的选择有密切关系，早期手术可使间接复位取得满意的效果。