二尖瓣脱垂的外科治疗

报道５７例二尖瓣脱垂（ＭＶＰ０不同术式的外科治疗效果。手术随机分为３组;传统的二尖瓣成形组,Ｇｏｒｅ－Ｔｅｘ线人工腱索替换组和瓣膜置换组。结果显示,Ⅱ组和Ⅲ组术后每搏二尖瓣返流量明显少于Ⅰ组。各组术后长期随访机能状况均明显改善。ＭＶＰ的外科治疗应首选二尖瓣成形;而Ｇｏｒｅ－Ｔｅｘ线人工腱索替换和人造瓣环盛开有方法安全可靠,其效果优于传统的腱索成形,并可避免瓣膜置换术后长期抗凝的并发症。     

U-Patch GAN: A Medical Image Fusion Method Based on GAN

Journal of Digital Imaging - Although medical imaging is frequently used to diagnose diseases, in complex diagnostic situations, specialists typically need to look at different modalities of image...     

P53与ras癌基因产物对直肠癌的诊断价值

目的提高直肠癌术前活检的诊断率。方法5年来手术治疗的168例直肠癌病人中,有20例（11．9％）第一次手术前活检不能明确诊断。对其中17例患者的活检组织石蜡标本（实验组）和10例正常肠粘膜活检标本（对照组）进行常规病理、P53与ras癌基因产物检测。结果实验组中P53与ras基因表达阳性率分别为71％与47％,阳性率均高于常规病理,有显著性差异。17例标本中,11例为不典型增生,5例仅有慢性炎症。不典型增生的P53与ras基因表达分别为11例和7例,而慢性炎症则全部为阴性。二者之间,两种基因表达阳性率均有显著差异。实验结果还显示P53与ras基因表达有较好的一致性。对照组的10例正常肠粘膜的活检标本P53与ras基因检测结果均阴性,与实验组比较,P53阳性率有显著差异。结论P53与ras基因表达检测,可以作为常规病理的补充,提高直肠良恶性病变的鉴别力。     

CART: from gene to function

CART was identified as a novel mRNA regulated by psychostimulant drugs. CART peptides appear to be neurotransmitters involved in a variety of functions such as feeding. The mouse gene has been characterized and localized to Chromosome 13. The processing of CART peptides is evident in Western blotting studies.     

Expression of intercellular adhesion molecule 1 (ICAM-1) is reduced in permanent focal cerebral ischemic mouse brain using an adenoviral vector to induce overexpression of interleukin-1 receptor antagonist

Our previous studies have demonstrated that overexpression of recombinant human interleukin-1 receptor antagonist protein (IL-1ra) via gene transfer can reduce ischemic brain injury. However, the mechanism of action of IL-1ra in ischemia is unclear. Since interleukin-1 can up-regulate intercellular adhesion molecules in endothelium, the present study was designed to determine whether overexpression of the IL-1ra can reduce the expression of intercellular adhesion molecule-1 (ICAM-1) after ischemic injury. Normal saline or adenovirus vector (1x109 particles) encoding the human IL-1ra gene (Ad.RSVIL-1ra) or the Escherichia coli LacZ gene (Ad.RSVlacZ) was injected into the right lateral cerebral ventricle of adult CD-1 mice. After five days, permanent middle cerebral artery occlusion (MCAO) was achieved for 24 h using an intraluminal suture. Cerebral blood flow was monitored by transcranial laser Doppler flowmetry to verify the occlusion. ICAM-1 protein was quantified using Western blot analysis and localized using immunohistochemistry. After MCAO, surface blood flow in the ischemic hemisphere was decreased to 9-11% of the baseline. There were fewer ICAM-1 positive vessels in the ischemic cortex of the Ad.RSVIL-1ra transfected mice than in the Ad.RSVlacZ transfected and saline treated mice (138+/-19 vs. 249+/-25, 284+/-22, p<0.05). Western blot analysis shows that ICAM-1 protein decreased 50-60% in the Ad. RSVIL-1ra group compared to the other two groups. There were no significant differences in the numbers of positive vessels in the ischemic basal ganglia and contralateral hemisphere among the three groups. Our studies suggest that IL-1ra overexpression can down-regulate the expression of ICAM-1 in the ipsilateral cortex in ischemic mice. Interleukin-1 may play an important role in the activation of inflammatory reaction during focal cerebral ischemia by promoting leukocyte adhesion on the endothelium cells.     

Intrathecal adenosine does not relieve allodynia-like behavior in spinally injured rats

The intrathecal (i.t.) administration of the adenosine A1-receptor agonist R-phenylisopropyl-adenosine (R-PIA) reduced pain-related behaviors after peripheral nerve or spinal cord injury in rats. The endogenous ligand adenosine is clinically available and has been tested i.t. as an analgesic. Thus, we set out to investigate whether i.t. adenosine could reduce allodynia in a model of central pain in spinally injured rats. I.t. adenosine did not reduce mechanical and cold allodynia-like behaviors at doses of 10, 100 and 187 nmol, whereas i.t. R-PIA at 10 nmol markedly alleviated allodynia in the same animals. The lack of effect by exogenous adenosine may be due to pharmacokinetic or pharmacodynamic reasons. Alternatively, adenosine may have reduced affinity and selectivity towards the A1-receptors which may be important for the antiallodynic effect.     

联合内分泌治疗对前列腺和睾丸的影响

目的 探讨抑那通和缓退瘤联合治疗对正常前列腺,增生的前列腺（ＢＰＨ）和前列腺癌以及睾丸的作用。方法 对１６例接受联合内分泌治疗至少３个月且有治疗前后病理资料的前列腺癌患者的标本进行了系统的病理学检查。对内分泌治疗后的睾丸标本与同龄未接受治疗的进行对照研究。结果 １４例内分泌治疗后的前列腺标本２例未见残存癌灶,９例对治疗有明显的反应;３例对治疗反应差,治疗并未降低前列腺癌的病理分期。３例内分泌治疗后     

膀胱癌上皮细胞粘附分子蛋白表达的意义

目的 探讨上皮细胞粘附分子（Ｅ－ＣＤ）表达情况对判断膀胱癌恶性程度、复发及预后的意义。方法 采用免疫组织化学方法对５４例膀胱癌标本中Ｅ－ＣＤ的表达情况进行研究。结果Ｅ－Ｃ原表达情况与膀胱癌的病理分级、分期均有密切关系。（ｘ＾２＝６．６５,Ｐ〈０．０５,Ｘ＾２＝７．１５,Ｐ〈０．０１）。Ｅ－ＣＤ的的民光癌的近期昨发及不良预后有关。（Ｘ＾２＝４．８８,Ｐ〈０．０５;Ｌｏｎｇ－Ｒａｎｋ ｔｅｓｔ;ｘ＾２     

我国农村地区实施大病统筹合作医疗或医疗保险方案的技术缺陷

在我国中等发达及上农村地区,大病统筹合作医疗或医疗保险以其可缓解农村居民的就医经济风险而受到欢迎,但是在方案的拟订过程中尚存在一些明显的技术缺陷,比如,“大病”的技术定义、农村居民就医经济风险分析、风险临界线界定、保险经付比测算和保险费率厘定等,这些技术的缺乏,已严重影响农村合作医疗或医疗保险的推广和实施效果。     

敏感期内、末单眼斜视和剥夺猫视觉系统脑源性神经营养因子表达机理研究

目的观察和研究敏感期内、末不同时限单眼斜视
(monocularstrabismus,MS)和单眼剥夺(monoculardeprivation,
MD)幼猫视皮质、外侧膝状体的脑源性神经营养因子(brain
derivedneurotrophicfactor,BDNF)的可塑性变化。从形态学、分
子神经生物学角度探讨不同时限MS和MD幼猫视