首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   182268篇
  免费   17320篇
  国内免费   11788篇
耳鼻咽喉   1630篇
儿科学   1905篇
妇产科学   2428篇
基础医学   21864篇
口腔科学   3259篇
临床医学   23898篇
内科学   26712篇
皮肤病学   2208篇
神经病学   9508篇
特种医学   6461篇
外国民族医学   97篇
外科学   18588篇
综合类   29589篇
现状与发展   39篇
一般理论   22篇
预防医学   12226篇
眼科学   4758篇
药学   19704篇
  189篇
中国医学   10932篇
肿瘤学   15359篇
  2024年   733篇
  2023年   2983篇
  2022年   7789篇
  2021年   9769篇
  2020年   7350篇
  2019年   6289篇
  2018年   6622篇
  2017年   6075篇
  2016年   5684篇
  2015年   8726篇
  2014年   10583篇
  2013年   9779篇
  2012年   14254篇
  2011年   15405篇
  2010年   9829篇
  2009年   7558篇
  2008年   10047篇
  2007年   9513篇
  2006年   9132篇
  2005年   8869篇
  2004年   5679篇
  2003年   5147篇
  2002年   4262篇
  2001年   3599篇
  2000年   3795篇
  1999年   3851篇
  1998年   2287篇
  1997年   2340篇
  1996年   1781篇
  1995年   1731篇
  1994年   1511篇
  1993年   907篇
  1992年   1280篇
  1991年   1115篇
  1990年   895篇
  1989年   842篇
  1988年   761篇
  1987年   623篇
  1986年   458篇
  1985年   396篇
  1984年   190篇
  1983年   162篇
  1982年   93篇
  1981年   123篇
  1980年   79篇
  1979年   108篇
  1978年   52篇
  1977年   51篇
  1976年   42篇
  1974年   44篇
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
PURPOSE: Perineural invasion is a frequent occurrence in salivary adenoid cystic carcinoma (ACC) and may prevent complete surgical resection. Studies have indicated that nerve growth factor (NGF) and its high-affinity receptor tyrosine kinase A (TrkA) may play a role in perineural invasion in several malignancies in which perineural invasion is observed. The present study was conducted to investigate the expression of NGF and TrkA in salivary ACC and to examine the effects of NGF on adhesion, migration and invasion capacities of a salivary ACC cell line (SACC-83) in vitro. PATIENTS AND METHODS: Expression of NGF and TrkA was explored using immunohistochemistry in paraffin-embedded tissues of 32 cases of salivary ACC. The effects of NGF on in vitro adhesion, migration, and invasion capacities of the SACC-83 cell line were examined using an MTT assay and a modified Boyden chamber assay respectively. RESULTS: In ACC specimens, 31 (96.9%) and 32 (100%) tumors showed immunoreactivity for NGF and TrkA respectively. Significant correlations were found between NGF/TrkA expression levels and perineural invasion (P < .05). In cell adhesion assay, the percent adherences of SACC-83 cells co-cultured with 25 ng/ml NGF at 1.5 hours and 5, 25 ng/ml NGF at 6 hours were significantly higher than that co-cultured with 0 ng/ml NGF (P < .05). However, high concentration of NGF (500 ng/ml) resulted in a significant inhibition of invasion (P < .05). CONCLUSION: Overexpression of NGF and TrkA in human salivary ACC tissues may constitute a reason for perineural invasion in salivary ACC.  相似文献   
102.
103.
本文对1984年1~3月和1985年3~8月共107例急性下呼吸道感染的住院患儿,采用间接免疫荧光法检测其急性期血清抗RSV特异性IgM抗体,并与病毒分离和/或中和试验比较,敏感性为82.1%,特异性为71.8%。RSV感染患儿发病后3天内大多数病例即可从血清中检测出RSV-IgM,因此该法具有早期诊断价值。  相似文献   
104.
105.
The purpose of investigation was to study the effect of intrathecal fentanyl on the onset and duration of hyperbaric bupivacaine-induced spinal block in adult male patients. Fortythree patients undergoing lower extremity or genitourinary surgery were enrolled to receive either 13.5 mg hyperbaric bupivacaine 0.75% + 0.5 ml CSF it, (Group I) or 13.5 mg hyperbaric bupivacaine 0.75% + 25 μg fentanyl it, (Group II) according to a randomized assessor-blind protocol. The onset and duration of sensory block were assessed by pinching the skin with forceps in the midclavicular line bilaterally every two minutes for first twenty minutes and then every five to ten minutes. Similarly, the onset and duration of motor block were assessed and graded at the same time intervals using the criteria described by Bromage. The time required for two sensory segment regression and sensory regression to L1 dermatome was 74 ± 18 and 110 ± 33 min vs 93 ± 22 and 141 ± 37 min in Groups I and II, respectively (P < 0.05). Intrathecal fentanyl did not enhance the onset of sensory or motor block, or prolong the duration of bupivacaine-induced motor spinal block. Fewer patients demanded pain relief in the fentanyl-treated group than in the control group in the early postoperative period (19% vs 59%; P < 0.05). Episodes of hypotension were more frequent in the fentanyl-treated group than in the control group (43% vs 14%; P < 0.05). We conclude that fentanyl, 25 μg it, prolonged the duration of bupivacaine-induced sensory block (sensory regression to L1 dermatone) by 28% and reduced the analgesic requirement in the early postoperative period following bupivacaine spinal block.  相似文献   
106.
雷公藤双层栓的研制   总被引:7,自引:0,他引:7  
介绍了雷公藤双层栓的制备和质量检查。雷公藤双层栓的上层为空白层,下层为含药层。空白层可有效地阻止后端所释药物向上扩散,从而避免相当部分药物经门肝系统吸收,因此可明显提高其生物利用度。  相似文献   
107.
本文报告动脉粥样硬化(AS)形成和消退研究的部分工作。在复制AS形成和消退模型的基础上,研究了AS形成和消退中的内皮细胞、平滑肌细胞和单核细胞的改变。  相似文献   
108.
R.R Fiscus  L Lu  A.W.K Tu  H Hao  L Yang  X Wang 《Neuropeptides》1998,32(6):499-509
Calcitonin gene-related peptide (CGRP) causes vasorelaxation in rat aorta involving endothelium/nitric oxide (NO)-dependent elevations of both cAMP and cGMP levels. When endothelium is removed, preincubation with exogenous NO uncovers and potentiates direct (endothelium-independent) cAMP elevations and vasorelaxations caused by CGRP. This enhancing effect of NO potentially involves elevation of cGMP and inhibition of Type III (cGMPinhibitable) phosphodiesterase, causing accumulation of cAMP. However, NO may have other actions. The aim of the present study was to determine if brain natriuretic peptide (BNP), which elevates cGMP levels independent of NO, could enhance cAMP accumulations and vasorelaxations induced by CGRP in rat aortic rings denuded of endothelium. When added separately, neither CGRP (100 nM) nor BNP (10 nM) altered cAMP levels. When added in combination, CGRP (100 nM) and BNP (10 nM) significantly elevated cAMP levels (from control of 0.95 ± 0.08 to 1.53 ± 0.09 pmol/mg protein) at 2 min. BNP (10 nM) elevated cGMP levels 10-fold at 2 min and this response was not altered by co-administration of CGRP (100 nM).Pretreatment with BNP at concentrations as low as 1 nM in endothelium-denuded aortic rings greatly enhanced the direct vasorelaxant effects of CGRP (100 nM) (from control of 0% to 57.6 ± 6.8% relaxation of phenylephrineprecontractions). Our findings indicate that BNP enhances direct (endothelium-independent) cAMP elevations and vasorelaxations caused by CGRP in rat aorta, thus supporting the concept that cGMP inhibits cAMP metabolism and enhances CGRP-induced responses in aortic smooth muscle cells.  相似文献   
109.
110.
用AR CM MIC阳离子测定系统,测量单个细胞内游离钙浓度([Ca2+]i),研究8-(N,N-二乙胺)-n-辛基 3,4,5-三甲氧基苯甲酸酯(TMB-8)对培养乳牛基底动脉平滑肌[Ca2+]i的作用。在细胞外钙浓度为1.3mmol·L-1时,TMB-8(30μmol·L-1)可明显抑制BHQ,NE及KCl引起[Ca2+]i的升高。在细胞外钙为零+EGTA 0.1mmol·L-1时,TMB-8(10,30及100μmol·L-1)可浓度依赖性地降低静息[Ca2+]i,TMB-8(30μmol·L-1)可几乎完全阻断BHQ及NE引起[Ca2+]i的增加。研究表明TMB-8降低培养乳牛基底动脉平滑肌[Ca2+]i的机制,主要是抑制肌浆网Ca2+的释放,或增加肌浆网对Ca2+的摄入,并由此间接地抑制细胞外钙的内流。  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号