中药类专业有机化学课程思政的探索

在课程思政教育背景下,从学生角度、教师角度和有机化学课程特点讨论开展课程思政的必要性,结合有机化学课程特点和教学实际情况提出有机化学课程思政的实施方法:教师转变教育理念,在课堂教学中运用案例法教学,在实验教学中贯彻绿色化学思想,建立线上线下混合的教学模式.为中药类专业开展有机化学课程思政提供参考.     

Analysis of circRNA‐mRNA expression profiles and functional enrichment in diabetes mellitus based on high throughput sequencing

To study the pathogenesis of diabetes mellitus (DM) and identify new biomarkers, high‐throughput RNA sequencing provides a technical means to explore the regulatory network of MD gene expression. To better elucidate the genetic basis of DM, we analysed the circRNA and mRNA expression profiles in serum samples from diabetic patients. The circRNAs and mRNAs with abnormal expression in the DM group and non‐diabetic group (NDM) were classified by RNA sequencing and differential expression analysis. The circRNA‐miRNA‐mRNA regulatory network reveals the mechanism by which competitive endogenous RNAs (ceRNAs) regulate gene expression. The biological functions and interactions of circRNA and mRNA were analysed by gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Differential expression analysis showed that 441 circRNAs (366 up‐regulated, 75 down‐regulated) and 683 mRNAs (354 up‐regulated, 329 down‐regulated) were significantly differentially expressed in the DM group compared with the NDM group. Screening of the differential genes at the nodes of the interaction network showed that a single circRNA could interact with multiple miRNAs and then jointly regulate more mRNAs. In addition, the expressions of circRNA CNOT6 and AXIN1 as well as mRNA STAT3, MYD88, and B2M were associated with the progression of diabetes. Enrichment pathway analysis indicated that differentially expressed circRNA and mRNA may participate in Nod‐like receptor signalling pathway, insulin signalling pathway, sphinolipid metabolism pathway, and ribosome pathway, and play a role in the pathogenesis of diabetes. This study provides a theoretical basis for elucidating the molecular mechanism of DM occurrence and development at circRNA and mRNA levels.     

Survivin蛋白在子宫内膜样腺癌中的表达及其临床意义

目的探讨Survivin蛋白在子宫内膜样腺癌中的表达及临床意义。方法15例正常子宫内膜组织、78例子宫内膜样腺癌组织,均采用免疫组化的方法检测Survivin蛋白。结果正常子宫内膜组织中,Survivin不表达或低表达。子宫内膜样腺癌组织中,Survivin表达水平升高。Survivin蛋白表达与临床分期、肿瘤细胞的分化程度、淋巴结转移有显著的相关性（所有P〈0．05）。结论子宫内膜样腺癌组织中Survivin蛋白表达上调,其在子宫内膜样腺癌的发生、发展中发挥着重要的作用,它有可能作为判断子宫内膜样腺癌预后的指标。     

针灸推拿联合腰部核心肌力训练在腰椎间盘突出症患者中的应用效果

目的 探讨针灸推拿联合腰部核心肌力训练对腰椎间盘突出症患者腰椎功能障碍指数（ODI）评分、腰背肌力及简式疼痛问卷表（MPQ）评分的影响。方法 选取2017年1月至2019年8月在辽宁省人民医院骨科接受治疗的136例腰椎间盘突出症患者作为研究对象,采用随机数字表法将其分为研究组（n=68）和对照组（n=68）。对照组患者采用腰部核心肌力训练治疗的方法,研究组患者在对照组基础上采用针灸推拿治疗的方法。比较两组患者的ODI评分、腰背肌力、MPQ各项评分及总有效率。结果 治疗后,两组患者的ODI评分均低于治疗前,差异有统计学意义（P<0.05）,且研究组患者的ODI评分低于对照组,差异有统计学意义（P<0.05）;治疗后,两组患者的腰背肌力均优于治疗前,差异有统计学意义（P<0.05）,且研究组患者的腰背肌力优于对照组,差异有统计学意义（P<0.05）;治疗后,两组患者的MPQ各项评分均低于治疗前,差异有统计学意义（P<0.05）,且研究组患者的MPQ各项评分低于对照组,差异有统计学意义（P<0.05）;治疗后,研究组患者的总有效率高于对照组,差异有统计学意...     

Addition of terlipressin to norepinephrine in septic shock and effect of renal perfusion: a pilot study

PurposeTerlipressin improves renal function in patients with septic shock. However, the mechanism remains unclear. Here, we aimed to evaluate the effects of terlipressin on renal perfusion in patients with septic shock.Materials and MethodsThis pilot study enrolled patients with septic shock in the intensive care unit of the tertiary hospital from September 2019 to May 2020. We randomly assigned patients to terlipressin and usual care groups using a 1:1 ratio. Terlipressin was intravenously pumped at a rate of 1.3 μg/kg/hour for 24 h. We monitored renal perfusion using renal contrast-enhanced ultrasound (CEUS). The primary outcome was peak sonographic signal intensity (a renal perfusion parameter monitored by CEUS) at 24 h after enrollment.Results22 patients were enrolled in this study with 10 in the terlipressin group and 12 in the usual care group. The baseline characteristics of patients between the two groups were comparable. The peak sonographic signal intensity at 24 h after enrollment in the terlipressin group (60.5 ± 8.6 dB) was significantly higher than that in the usual care group (52.4 ± 7.0 dB; mean difference, 7.1 dB; 95% CI, 0.4–13.9; adjusted p = .04). Patients in the terlipressin group had a lower time to peak, heart rates, norepinephrine dose, and a higher stroke volume at 24 h after enrollment. No significant difference in the urine output within 24 h and incidence of acute kidney injury within 28 days was found between the two groups.ConclusionsTerlipressin improves renal perfusion, increases stroke volume, and decreases norepinephrine dose and heart rates in patients with septic shock.     

胃黏膜病变组织COX-2蛋白表达的研究

目的：研究胃黏膜病变组织中环氧化酶－2（COX－2）蛋白的表达，探讨其与胃癌类型，分期，分化，转移及幽门螺杆菌（Hp)感染之间的内在关系。方法：外科手术中取30例胃癌者的癌组织，癌旁非癌组织各两块，石蜡包埋，切片HE染色作病理及免疫组织化学检查COX－2蛋白表达，Hp阳性感染由快速尿素酶试验结合病理染色／^14C尿素呼气试验而确立。结果：COX－2在胃癌中阳性表达率（66．7％），癌旁组织（26．7％），差异具有显著性（P＜0．01），其表达强度差异也有非常显著性意义（P＜0．001）。COX－2蛋白阳性表达率；进展期胃癌（76．0％）显著高于早期胃癌（20．0％）（P＜0．05）；有淋巴结转移（79．2％）显著高于无淋巴结转移（16．7％）（P＜0．05）；肠型胃癌（66．7％）与胃型胃癌（66．7％），低，未分化癌（80．0％）与高、中分化癌（57．1％），相比统计学上均无显著性差异（P＞0．05）。Hp阳性肠化或异型增生组COX－2的表达显著高于Hp阴性组（P＜0．05）。结论：COX－2蛋白在胃癌有较高水平的表达，它的异常表达多与胃癌的进展及淋巴结转移有关而与胃癌类型，病理学上分化程度关系上。COX－2基因在胃癌早期发生发展过程中可能起重要的作用，并与Hp感染有一定关系。     

颅内巨大动脉瘤的手术治疗

目的：颅内巨大动脉瘤虽然少见,但手术治疗困难,手术病死率和致废率仍较高。本对我院1980年以来手术治疗的30例颅内巨大动脉瘤作一回顾性分析。方法：动脉瘤瘤颈夹闭术13例,动脉瘤孤立后切除术11例;动脉瘤切除或孤立后载瘤动脉重建术2例;颈部颈内动脉结扎术2例;动脉瘤包裹术2例。结果 术后早期恢复良好为73．3％,中、重残为20％,死亡2例,病死率6．6％。动脉瘤瘤颈夹闭组的13例病人中、除1例术前Ⅳ级的前交通动脉瘤病人术后重残外,均恢复顺利。动脉瘤孤立或孤立后切除的11例病人中,4例术后早期出现不同程度的对侧肢体瘫痪,其中2例在术后3周内恢复正常,另2例分别在术后3个月和28个月恢复正常;1例大脑中动脉（MCA）动脉瘤术后因术野血肿再次手术清除血肿后,病人出院时能独立行走。单纯行颈部颈动脉结扎的2例病人出院时病情无明显变化。而仅行动脉瘤包裹术的2例病人均在术后因再出血死亡。结论 为消除巨大动脉瘤的占位效应和出血的潜在性,对颅内巨大动脉瘤提倡积极的直接手术治疗。动脉瘤瘤颈直接夹闭并保留载瘤动脉通畅是首选治疗,对必需行动瘤孤立且交叉循环不良的病例,应尽可能重建载瘤动脉。     

UPLC-Q-TOF MS/MS对痛安注射液指纹图谱中化学成分的研究

目的：采用液相与四极杆飞行时间串联质谱（UPLC-QTOF-MS/MS）对指纹图谱中色谱峰进行研究,对痛安注射液中的生物碱类化学成分进行定性鉴别。方法：采用Agilent SB-C18 RRHD（3 mm×100 mm,1.8μm）色谱柱,以乙腈-0.1%甲酸为流动相进行梯度洗脱,流速0.4 mL·min-1,柱温为25℃,进样量为2μL。检测质谱采用电喷雾离子源（ESI）的飞行时间质谱,正离子模式下采集数据,一级质谱质荷比扫描范围m/z 100～1500,二级质谱质荷比扫描范围m/z 30～500。结果：痛安注射液的指纹图谱色谱峰中共鉴别出12个成分。结论：本方法快速、准确地鉴定了痛安注射液的指纹图谱中的主要成分,为痛安注射液质量标准的完善提供依据。     

排毒养颜胶囊对非酶体系产生的·OH及的清除作用

目的 :通过对活性氧自由基的清除作用研究排毒养颜胶囊排毒养颜的部分作用机理。方法 :用化学发光法测定该中药方剂水浸提液对非酶体系产生的超氧阴离子自由基 (O ·2 )和羟自由基 (·OH)的清除作用。结果 :该浸提液清除O ·2 的SC5 0 为 2 7× 1 0 5 g·ml 1 ,相当于 1 0ng·ml 1 SOD的活性 ;清除·OH的SC5 0 为 6 5× 1 0 5 g·ml 1 ,相当于 1× 1 0 5 mol·L 1 硫脲的活性。当浸提液浓度较高时 ,它对O ·2 和·OH的清除率均可达 90 %以上。结论 :这种较强的抗自由基活性可能是该中药方剂排毒养颜的部分作用机理     

常见的化学性食物中毒的判断及预防

文章中介绍了常见的化学性食物中毒的毒素及中毒症状,针对每一种化学性食物中毒提出相应预防措施,并陈述发生食物中毒的应急处理措施。