人卵巢癌P53基因的突变

目的：了解人卵巢癌中Ｐ５３基因突变的特点及其与临床分期的关系。方法：采用聚合酶链反应单链构像多态性分析及序列分析，对６０例卵巢癌组织及癌旁组织Ｐ５３基因外显子５、６、７进行了筛查。结果：所有癌旁组织均未发现突变，癌组织有９例发生了Ｐ５３基因的突变，并对其中５例进行了序列分析，证实了其突变的存在。５例测序标本中有４例发生了Ｇ∶Ｃ→Ａ∶Ｔ的转换突变。９例阳性标本分别为临床Ⅰ期２例，临床Ⅲ期６例，临床Ⅳ期１例。结论：人卵巢癌中Ｐ５３基因的突变是自发性突变的结果。Ｐ５３基因改变可能发生于卵巢癌早期，并持续存在于肿瘤发展的全过程，是卵巢癌发生发展的危险因素之一。推测２４５位密码子是我国卵巢癌患者的一个突变热点。     

抑癌基因P16表达与肺癌患者预后

目的：观察Ｐ１６蛋白在肺癌组织的表达情况，了解该基因与肺癌的预后关系。方法：应用ＳＰ免疫组织化学方法对７６例肺癌中抑癌基因ＣＤＫＮ２Ｐ１６蛋白表达进行研究。结果：７６例肺癌Ｐ１６蛋白表达阳性率为４４７％。Ｐ１６蛋白表达与肺癌的病理分级及预后有显著差异（Ｐ＜００５），与临床分期有一定相关性。结论：Ｐ１６抑癌基因对肺癌的发生发展起着某种关键作用     

大株红景天注射液治疗心绞痛的Meta分析

目的 评价大株红景天注射液治疗心绞痛的疗效和安全性。方法 计算机检索Medline、Embase、The Cochrane Library和CNKI、VIP、万方等文献数据库,按纳入和排除标准纳入合格的随机对照试验文献,并对其进行质量评价和meta分析。结果 符合纳入标准的文献共有14篇,包括1 616例患者,但这些研究的质量普遍不高。Meta分析结果显示,大株红景天注射液与对照组心绞痛临床症状疗效和心电图改善的合并比值比(odd ratio,OR)分别为2.29和2.16,95%的可信区间(CI)分别为(1.78,2.96)和(1.69,2.76);大株红景天注射液治疗心绞痛,临床症状疗效和心电图改善优于对照组,但无可靠证据显示该药的安全性。结论 目前,仅有少量证据支持大株红景天注射液作为西医基础治疗的辅助药物时在改善各类心绞痛症状和心电图表现方面优于单纯西医基础治疗,由于纳入研究的数量少和质量不高,且缺乏一些重要的终点指标和生活质量相关指标,尚不能从本系统评价得出大株红景天注射液治疗心绞痛优于单纯西医基础治疗的结论。     

A novel riboflavin gastro-mucoadhesive delivery system based on ion-exchange fiber

A novel gastro-mucoadhesive delivery system based on ion-exchange fiber has been developed. Riboflavin-5'-phosphate sodium salt (RF5P), which is site-specifically absorbed from the upper gastrointestinal tract, was used as model drug. A modified dissolution system, which can also be called 'flow through diffusion cell' (FTDC), was used to study the drug release from the drug fibers. Gastrointestinal transit studies of the RF5P fiber complexes in rats and gamma imaging study in volunteer was carried out to evaluate the gastro-retentive behavior of the fiber. The pharmacokinetic profile and parameters of riboflavin via analysis of urinary excretion of riboflavin on man were measured. Study on rat and man provide evidence for the validity of the hypothesis that the drug fiber provided good mucoadhesive properties in vivo and should therefore be of considerable interest for the development of future mucoadhesive oral drug delivery dosage forms.     

Qualitative and quantitative analysis of four species of Curcuma rhizomes using twice development thin layer chromatography

The rhizomes of Curcuma phaeocaulis, Curcuma kwangsiensis, Curcuma wenyujin and Curcuma longa are used as Ezhu or Jianghuang in traditional Chinese medicine for a long time. Due to their similar morphological characters, it is difficult to distinguish their origins of raw materials used in clinic. In this study, a simple, rapid and reliable twice development TLC method was developed for qualitative and quantitative analysis of the four species of Curcuma rhizomes. The chromatography was performed on silica gel 60F(254) plate with chloroform-methanol-formic acid (80:4:0.8, v/v/v) and petroleum ether-ethyl acetate (90:10, v/v) as mobile phase for twice development. The TLC markers were colorized with 1% vanillin-H(2)SO(4) solution. The four species of Curcuma were easily discriminated based on their characteristic TLC profiles, and simultaneous quantification of eight compounds, including bisdemethoxycurcumin, demethoxycurcumin, curcumine, curcumenol, curcumol, curdione, furanodienone and curzerene, in Curcuma were also performed densitometrically at lambda(scan)=518nm and lambda(reference)=800 nm. The investigated compounds had good linearity (r(2)>0.9905) within test ranges. Therefore, the developed TLC method can be used for quality control of Curcuma rhizomes.     

Characterization of a novel and selective cannabinoid CB1 receptor inverse agonist, Imidazole 24b, in rodents

We document in vitro and in vivo effects of a novel, selective cannabinoid CB(1) receptor inverse agonist, Imidazole 24b (5-(4-chlorophenyl)-N-cyclohexyl-4-(2,4-dichlorophenyl)-1-methyl-imidazole-2-carboxamide). The in vitro binding affinity of Imidazole 24b for recombinant human and rat CB(1) receptor is 4 and 10 nM, respectively. Imidazole 24b binds to human cannabinoid CB(2) receptor with an affinity of 297 nM; in vitro, it is a receptor inverse agonist at both cannabinoid CB(1) and CB(2) receptors as it causes a further increase of forskolin-induced cAMP increase. Oral administration of Imidazole 24b blocked CP-55940-induced hypothermia, demonstrating cannabinoid CB(1) receptor antagonist efficacy in vivo. Using ex vivo autoradiography, Imidazole 24b resulted in dose-dependent increases in brain cannabinoid CB(1) receptor occupancy (RO) at 2h post-dosing in rats, indicating that approximately 50% receptor occupancy is sufficient for attenuation of receptor agonist-induced hypothermia. Imidazole 24b administered to C57Bl/6 mice and to dietary-induced obese (DIO) Sprague-Dawley rats attenuated overnight food intake with a minimal effective dose of 10 mg/kg, p.o. Administration had no effect in cannabinoid CB(1) receptor-deficient mice. DIO rats were dosed orally with vehicle, Imidazole 24b (1, 3 or 10 mg/kg), or dexfenfluramine (3 mg/kg) for 2 weeks. At 3 mg/kg, Imidazole 24b reduced cumulative food intake, leading to a non-significant decrease in weight gain. Imidazole 24b at 10 mg/kg and dexfenfluramine treatment inhibited food intake and attenuated weight gain. These findings suggest that selective cannabinoid CB(1) receptor inverse agonists such as Imidazole 24b have potential for the treatment of obesity.     

~(99m)Tc-葡聚糖乳内淋巴显像及其临床评价

本文报告26例正常人和35例乳腺包块~(99m)Tc-葡聚糖乳内淋巴显像的结果。正常人绝大多数双侧淋巴结数和(或)显影浓淡不对称,并发现左右交叉引流和先天性单链各2例;乳癌乳内淋巴转移者双侧或单侧淋巴链不显影,或一侧淋巴链仅低位淋巴结显影而高位缺如。本法判断淋巴受累的灵敏度为100％,特异性为85.2％,准确性为88.6％,假阳性为14.8％,假阴性为零。     

骨髓增生异常综合征(附33例临床分析)

本文分析我院33例MDS病例的入院前诊断、病例类型、临床表现、血象及骨髓象资料,其中4例转变为急性白血病。     

儿童中11种(型)军团菌感染情况的研究

本文报告南京地区110例肺炎病儿的双份血清和533份非呼吸系疾有病儿血清的L_pl-8和Lm、Ld、Ld、Lg的MA抗体流行情况。肺炎病儿中军团菌肺炎占5.45％,感染菌型分别为Lpl、3、7、8和Ld。此外,对军团菌的感染谱、抗体消长特点和儿童军团菌抗体正常上眼滴度等问题也进行了讨论。     

RP-HPLC测定地佐辛的含量

目的:建立地佐辛含量测定的反相高效液相色谱法(RP-HPLC)。方法:色谱柱为SHIMADZV VP-ODS(250 mm×4.6 mm;5μm),流动相为乙腈-三乙胺溶液(20∶80),检测波长:281 nm,流速:1.0 m L/min,柱温:30℃,进样量:20μL。结果:地佐辛在0.05~0.30 mg/m L范围内线性关系良好,r=1.000 0;平均回收率为100.2%(RSD=1.0%)。结论:该法操作简便、结果准确,可以用于地佐辛的定量分析。