全文获取类型
收费全文 | 1539篇 |
免费 | 81篇 |
国内免费 | 32篇 |
专业分类
耳鼻咽喉 | 3篇 |
儿科学 | 75篇 |
妇产科学 | 32篇 |
基础医学 | 151篇 |
口腔科学 | 22篇 |
临床医学 | 159篇 |
内科学 | 397篇 |
皮肤病学 | 88篇 |
神经病学 | 116篇 |
特种医学 | 134篇 |
外科学 | 122篇 |
综合类 | 74篇 |
一般理论 | 3篇 |
预防医学 | 69篇 |
眼科学 | 28篇 |
药学 | 78篇 |
中国医学 | 1篇 |
肿瘤学 | 100篇 |
出版年
2022年 | 9篇 |
2021年 | 22篇 |
2020年 | 18篇 |
2019年 | 19篇 |
2018年 | 38篇 |
2017年 | 27篇 |
2016年 | 40篇 |
2015年 | 38篇 |
2014年 | 51篇 |
2013年 | 91篇 |
2012年 | 54篇 |
2011年 | 76篇 |
2010年 | 82篇 |
2009年 | 56篇 |
2008年 | 50篇 |
2007年 | 86篇 |
2006年 | 55篇 |
2005年 | 51篇 |
2004年 | 47篇 |
2003年 | 41篇 |
2002年 | 41篇 |
2001年 | 23篇 |
2000年 | 15篇 |
1999年 | 21篇 |
1998年 | 57篇 |
1997年 | 50篇 |
1996年 | 41篇 |
1995年 | 29篇 |
1994年 | 33篇 |
1993年 | 36篇 |
1992年 | 11篇 |
1991年 | 9篇 |
1990年 | 15篇 |
1989年 | 41篇 |
1988年 | 27篇 |
1987年 | 25篇 |
1986年 | 17篇 |
1985年 | 11篇 |
1984年 | 11篇 |
1983年 | 10篇 |
1982年 | 11篇 |
1981年 | 13篇 |
1980年 | 13篇 |
1976年 | 7篇 |
1975年 | 7篇 |
1973年 | 5篇 |
1963年 | 6篇 |
1962年 | 5篇 |
1960年 | 6篇 |
1941年 | 11篇 |
排序方式: 共有1652条查询结果,搜索用时 15 毫秒
91.
Arguably, Australia's most significant judicial pronouncement on the human rights of those with mental illnesses was made in 2009 by Justice Bell, the then President of the Victorian Civil and Administrative Tribunal (“VCAT”). The decision is an exhaustive analysis of the application of the Charter of Human Rights and Responsibilities Act 2006 (Vic) to the involuntary status of a person subject to a community treatment order in Victoria. It occurred in the context of delays in the conduct of reviews of the status of a mentally ill person by Victoria's Mental Health Review Board (“the Board”). The outcome of the hearing was a declaration that the Board had breached the person's human rights to a fair hearing, even though the person's involuntary status on a community treatment order was not disturbed by VCAT. Whilst some important aspects of Justice Bell's decision concerning the general methodology to be applied when analysing human rights were overturned in the subsequent Court of Appeal decision of R v Momcilovic [2010] VSCA 50, the latter decision did not concern mental health and so leaves Kracke as the most detailed articulation and analysis of human rights within this difficult sphere. 相似文献
92.
Arg-Gly-Asp-dependent occupancy of GPIIb/IIIa by applaggin: evidence for internalization and cycling of a platelet integrin 总被引:1,自引:0,他引:1
Using indirect immunofluorescence microscopy we examined the distribution and cycling of GPIIb/IIIa after binding to applaggin, a high-affinity Arg-Gly-Asp (RGD)--containing ligand. Resting, unfixed platelets were incubated with applaggin for 30 minutes at 37 degrees C, and bound applaggin was detected by an affinity-purified rabbit anti- applaggin antibody. Examination of intact cells showed a rim pattern for applaggin, consistent with its binding to the platelet surface. Staining of Triton X-100--permeabilized cells showed an intracellular pool of applaggin. Competition of applaggin binding by either AP-2, an anti-GPIIb/IIIa monoclonal antibody (MoAb) that blocks fibrinogen binding, or the synthetic peptide RGDW eliminated both surface and intracellular staining, indicating that applaggin is binding to GPIIb/IIIa in an RGD-dependent manner. Inhibition of platelet activation by PGE1 and theophylline had no effect on the observed staining patterns, indicating that cellular activation is not required for surface binding and subsequent internalization. To evaluate whether occupancy of functional binding sites on GPIIb/IIIa is required for internalization, we used mAb15, an anti-GPIIIa antibody that neither blocks fibrinogen binding nor induces the expression of ligand-induced binding sites on GPIIb/IIIa. In these studies mAb15 was internalized in a manner analogous to both AP-2 and applaggin, showing that occupancy of the RGD binding site is not required to initiate receptor internalization. To estimate the size of the newly internalized pool of applaggin, 125I-applaggin--binding studies were performed. Displacement of bound 125I-applaggin by excess unlabeled applaggin or EDTA showed that at least 17% of bound applaggin was nondisplaceable when binding was performed under conditions permitting membrane flow and internalization. These data indicate that GPIIb/IIIa is internalized in unstimulated platelets independent of cellular activation or occupancy of the functional binding site(s) of GPIIb/IIIa by RGD-containing ligands. Thus, internalization of GPIIb/IIIa may represent a mechanism by which the surface expression of this adhesion receptor is regulated. 相似文献
93.
Choon Jin Ooi Ida Hilmi Rupa Banerjee Sai Wei Chuah Siew Chien Ng Shu Chen Wei Govind K Makharia Pises Pisespongsa Min Hu Chen Zhi Hua Ran Byong Duk Ye Dong Il Park Khoon Lin Ling David Ong Vineet Ahuja Khean Lee Goh Jose Sollano Wee Chian Lim Wai Keung Leung Raja Affendi Raja Ali Deng Chyang Wu Evan Ong Nazri Mustaffa Julajak Limsrivilai Tadakazu Hisamatsu Suk Kyun Yang Qin Ouyang Richard Geary Janaka H De Silva Rungsun Rerknimitr Marcellus Simadibrata Murdani Abdullah Rupert W L Leong 《Journal of gastroenterology and hepatology》2019,34(8):1296-1315
The Asia–Pacific Working Group on Inflammatory Bowel Disease was established in Cebu, Philippines, under the auspices of the Asia–Pacific Association of Gastroenterology with the goal of improving inflammatory bowel disease care in Asia. This consensus is carried out in collaboration with Asian Organization for Crohn's and Colitis. With biologic agents and biosimilars becoming more established, it is necessary to conduct a review on existing literature and establish a consensus on when and how to introduce biologic agents and biosimilars in conjunction with conventional treatments for ulcerative colitis and Crohn's disease in Asia. These statements also address how pharmacogenetics influences the treatments of ulcerative colitis and Crohn's disease and provides guidance on response monitoring and strategies to restore loss of response. Finally, the review includes statements on how to manage treatment alongside possible hepatitis B and tuberculosis infections, both common in Asia. These statements have been prepared and voted upon by members of inflammatory bowel disease workgroup employing the modified Delphi process. These statements do not intend to be all‐encompassing, and future revisions are likely as new data continue to emerge. 相似文献
94.
Interleukin-3, GM-CSF, and TPA induce distinct phosphorylation events in an interleukin 3-dependent multipotential cell line 总被引:9,自引:0,他引:9
The mechanism of action of the hemopoietic growth factor, murine interleukin-3 (mIL-3), was investigated using an mIL-3-dependent multipotential hematopoietic cell line, B6SUtA1. Murine granulocyte- macrophage colony-stimulating factor (mGM-CSF) was as potent as mIL-3 in stimulating these cells. In addition, sodium orthovanadate, an inhibitor of phosphotyrosine phosphatase, and 12-O-tetradecanoyl- phorbol-13-acetate (TPA), a known activator of protein kinase C, also stimulated DNA synthesis in these cells, suggesting that protein phosphorylation might be involved in the mechanism of action of mIL-3 and mGM-CSF. To assess this possibility, intact B6SUtA1 cells exposed for brief periods to mIL-3, mGM-CSF, and TPA were analyzed for changes in phosphorylation patterns using metabolic 32P-labeling and antibodies to phosphotyrosine. Both mIL-3 and mGM-CSF induced the serine-specific phosphorylation of a 68-Kd cytosolic protein, whereas all three agents stimulated the serine-specific phosphorylation of a 68-Kd membrane protein. Furthermore, mIL-3 stimulated tyrosine phosphorylation of the 68-Kd membrane protein, as well as of 140-, 90-, 55, and 40-Kd proteins. The 90-Kd protein was also tyrosine phosphorylated in response to mGM-CSF. These phosphotyrosine containing proteins were not detected in TPA-treated cells. These results indicate that protein phosphorylations on tyrosine and serine residues occur in B6SUtA1 cells following short-term incubation with mIL-3 or mGM-CSF and that most of these phosphorylation events are mediated by kinases other than protein kinase C (PkC). 相似文献
95.
BDSS Budagoda KAS Kodikara WKS Kularatne RM Mudiyanse DH Edussuriya JP Edirisinghe IP Karunaratne KGAD Weerakoon SC Medagedara SAM Kularatne 《Asian Pacific journal of tropical medicine》2010,3(7):586-588
The sting of Giant Asian honeybee (Apis dorsata) or Bambara in Sinhala and Karunge Kulavi in Tamil is a common environmental hazard in Sri Lanka known to cause immediate allergic reactions, which could be fatal in sensitized individuals. We reported myocardial infarction, bowel gangrene and fatal anaphylaxis in a prospectively proven case series and the association of these uncommon complications with delayed removal of stingers from the patients' skin. 相似文献
96.
97.
Two hundred and fifty eight newborn babies were studied for the presence of retinal hemorrhages between 1-3 days of birth. The overall incidence of retinal hemorrhages was found to be 18.9%. It was observed that the incidence of retinal hemorrhages was higher in unassisted vaginal deliveries than in assisted births. Also, a two fold higher incidence was noted in term infants as compared to preterm babies. No association was seen with birth asphyxia. 相似文献
98.
Teresa A Gilewski Govind Ragupathi Maura Dickler Shemeeakah Powell Sonal Bhuta Kathy Panageas R Rao Koganty Jeannette Chin-Eng Clifford Hudis Larry Norton Alan N Houghton Philip O Livingston 《Clinical cancer research》2007,13(10):2977-2985
PURPOSE: To determine the clinical toxicities and antibody response against sTn and tumor cells expressing sTn following immunization of high-risk breast cancer patients with clustered sTn-KLH [sTn(c)-KLH] conjugate plus QS-21. EXPERIMENTAL DESIGN: Twenty-seven patients with no evidence of disease and with a history of either stage IV no evidence of disease, rising tumor markers, stage II (>or=4 positive axillary nodes), or stage III disease received a total of five injections each during weeks 1, 2, 3, 7, and 19. Immunizations consisted of sTn(c)-KLH conjugate containing 30, 10, 3, or 1 microg sTn(c) plus 100 microg QS-21. Induction of IgM and IgG antibodies against synthetic sTn(c) and natural sTn on ovine submaxillary mucin were measured before and after therapy. Fluorescence-activated cell sorting analyses assessed reactivity of antibodies to LSC and MCF-7 tumor cells. RESULTS: The most common toxicities were transient local skin reactions at the injection site and mild flu-like symptoms. All patients developed significant IgM and IgG antibody titers against sTn(c). Antibody titers against ovine submaxillary mucin were usually of lower titers. IgM reactivity with LSC tumor cells was observed in 21 patients and with MCF-7 cells in 13 patients. There was minimal IgG reactivity with LSC cells. CONCLUSION: Immunization with sTn(c)-KLH conjugate plus QS-21 is well tolerated and immunogenic in high-risk breast cancer patients. Future trials will incorporate sTn(c) as a component of a multiple antigen vaccine. 相似文献
99.
K-Ⅱ系k阿片激动剂U-50488的同类物。通过部分离体和整体实验比较了K-Ⅱ与U-50488的药理作用。实验发现,K-Ⅱ抑制电刺激兔输精管收缩的IC50值为0.42 nmol/L,U-50488为26.5 nmol/L;K-Ⅱ抑制小鼠运动功能(横筛法)的ED50值为1.7 mg/g,U-50488为15.3 mg/kg;K-Ⅱ的小鼠LD50值为152.5 mg/kg,U-50488为118.4 mg/g;K-Ⅱ明显降低小鼠自发活动的作用比U-50488强5倍。结果表明,K-Ⅱ是一个药理作用较U-50488强的k受体激动剂。 相似文献
100.