Isolation and characterization of fifteen microsatellite loci in Leopard grouper (Mycteroperca rosacea) via 454 pyrosequencing

Leopard grouper (Mycteroperca rosacea) are distributed from Bahía Magdalena, through the Gulf of California and as far south as Jalisco, Mexico. They are listed as Vulnerable on the IUCN Red List due to significant fishing-induced declines over the past decade. Microsatellite loci were isolated by constructing a shotgun genomic library and sequencing using 454 XL + titanium chemistry. We characterized 15 polymorphic loci in 120 samples from three geographic locations. Genotyping yielded 15–60 alleles per locus with observed levels of heterozygosity ranging from 0.575 to 0.975.     

Laboratory Aspirin Resistance Reversibility in Diabetic Patients: a Pilot Study Using Different Pharmaceutical Formulations

Purpose

Aspirin resistance occurs most frequently in diabetic patients and is associated with poor prognosis. The purpose of this study was to evaluate the prevalence of aspirin resistance in a cohort of diabetic patients and whether it can be reversed using more bioavailable aspirin formulations.

Methods

Platelets function of 163 diabetic patients taking acetyl salicylic acid (ASA) 100 mg daily has been evaluated with PFA100 and VerifyNow. Patients found resistant by at least one test received an infusion of 288 mg of lysine acetylsalicylate (Flectadol®) corresponding to ASA 160 mg. Platelets function was measured again after 1 and 24 h. Patients whose the resistance was reversed received 288 mg of soluble salt of lysine acetylsalicylate (Cardirene 160®) corresponding to ASA160 mg instead of aspirin and their aggregation status was re-evaluated after 1 month of therapy.

Results

Prevalence of aspirin resistance in our population was 18,4 % (30/163). In 27 out of 30 patients (90 %) aspirin resistance was reversed within 24 h from the infusion. 25 out of 27 patients (92 %) were found fully aspirin-sensitive after 1 month of oral therapy with soluble salt; two patients were found with borderline value. No adverse reactions were observed.

Conclusions

A significant number of diabetic patients are resistant to aspirin therapy. A single intravenous dose of lysine acetylsalicylate can reverse the platelet hyper-aggregability and laboratory aspirin resistance in large majority of patients. The efficacy of antiaggregation can be maintained by chronic therapy with an oral drug with a more favourable pharmacokinetic profile.     

Thirty-six consecutive reverse shoulder arthroplasties in cuff arthropathy: our experience with the anterosuperior approach

Background

The purpose of this study was to analyze the results obtained with the anterosuperior approach with reverse shoulder arthroplasty in terms of functional recovery, pain, and stability of the implant.

Methods

In a cohort of 33 patients with cuff arthropathy, 36 consecutive reverse shoulder arthroplasties were implanted by the same surgeon, using the anterosuperior approach, saving the subscapularis tendon. Those cases were evaluated preoperatively and postoperatively after an average of 37 months on the basis of constant score, X-ray evaluation, and incidence of dislocation.

Results

The constant score increased from 30.4 points preoperatively to 68.3 points. No case of instability was observed.

Conclusions

The good functional results observed with a mean follow-up of approximately 3 years doubtlessly resulted from the correct surgical technique on the glenoid component and the preservation of the anterior wall composed of the subscapularis tendon. Analyzing literature and taking into account our experience, we can say that the anterosuperior approach has certain advantages; this approach takes advantage of the absence of the upper part of the rotator cuff to facilitate exposure of the joint that makes it particularly indicated in cuff arthropathy.