Photodynamic therapy induced Fas-mediated apoptosis in human carcinoma cells

Photodynamic therapy (PDT) is a clinical approach that utilizes light-activated drugs for the treatment of a variety of pathologic conditions. Human poorly (CNE2) and moderately differentiated (TW0-1) human nasopharyngeal carcinoma (NPC) cells undergo rapid apoptosis when treated with PDT sensitized with Hypocrellin A (HA) and Hypocrellin B (HB). It has been shown that these compounds have a strong photodynamic effect on tumors and viruses. The initiating events of PDT sensitized HA and HB-induced apoptosis are poorly defined. In the current study, we sought to determine whether Fas/FasL upregulation and involvement of mitochondrial events are an early event in HA and HB-treated PDT induced apoptosis. Loss of mitochondrial transmembrane potential, release of cytochrome c, involvement of caspases-8 and -3 and the status caspase-3 specific substrate PARP, were evaluated in PDT treated tumor cells. Photoactivation of HA and HB enhanced both CD95/CD95L expression and induced CD95-signaling dependent cell death in all tumor cell lines studied. CD95/ CD95L expression appeared within 2 h following light activation and appeared to be a primary event in PDT induced apoptosis. Furthermore, these results indicate that release of mitochondrial cytochrome c into the cytoplasm is a secondary event following the activation of initiator caspase-8 preceding caspase-3 activation, cleavage of PARP and DNA fragmentation. Cytochrome c appeared in the cytosol within 2-3 h post PDT. Cleavage of PARP was observed at 3-4 h following PDT and caspase-3 specific inhibitor DEVD-CHO and broad-spectrum caspases inhibitor z-VAD-fmk blocked caspase-3 activation and PARP cleavage suggesting that caspase-3 plays an important role in HA and HB-induced apoptosis.     

BT—BAK细胞和BCG膀胱腔内过继免疫治疗对膀胱癌患者全身免疫功能影响的比较

目的：观察BT-BAK细胞与BCG膀胱腔内灌注治疗对患者全身免疫功能的影响。方法：由手术切除的膀胱瘤标本制备膀胱肿瘤可溶性抗原,以BCG为佐剂,从PBMNC中诱导出抗膀胱肿瘤特异性细胞毒性BT-BAK细胞,临床选择72例浅表性膀胱癌术后患者随机平均分成2组分别进行BT-BAK和BCG的膀胱腔内灌注治疗。于不同阶段采用ELISA法测定患者外周血中IL-2、TNF-α及IFN-γ的含量;观察治疗后患者PBMNC对T24膀胱肿瘤细胞株杀伤活性的变化。结果：BT-BAK组患者血清中IL-2、TNF-α及IFN-γ的水平均较治疗前明显提高（P＜0.01);患者PBMNC对T24膀胱肿瘤细胞株的杀伤活性显著增强（P＜0.01)。BT-BAK组上述指标均高于BCG组（P＜0.05)。平均随访18.16个月,两组的复发率分别为2.7%和11.11%。结论：BT-BAK细胞及其培养上清进行膀胱腔内灌注治疗,能够有效提高膀胱癌术后患者的全身免疫水平,降低膀胱癌的术后复发率。     

酒客乐对酒精性肝损伤大鼠血清微量元素含量的影响

目的 :观察酒客乐对酒精性肝损伤大鼠血清微量元素含量的影响。方法 :用白酒、吡唑、橄榄油的混合液制成酒精性肝损伤模型 ,酒客乐冲剂为防治用药 ,观察血清微量元素Cu、Zn、Se及Mn含量的变化。结果 :与正常组比较 ,肝损伤组血清Se、Cu、Zn含量下降 (P <0 0 1或P <0 0 5 ) ;而防治组血清Se、Cu、Zn含量得以回升 ,其中Zn含量升至 3 2± 0 2 4ug ml,明显高于损伤组 (P <0 0 1)。结论 :酒客乐可通过补充酒精性肝损伤大鼠某些血清微量元素含量间接发挥清除自由基 ,稳定生物膜的作用     

硅凝胶型乳房假体凝胶外渗的实验研究

目的 :研究硅凝胶型乳房假体植入大鼠体内后凝胶外渗对机体的影响。方法 :取 40只健康SD雌性大鼠分成A、B、C、D 4组 ,A、B、C 3组分别植入实验用完整的硅凝胶型假体、刺破的硅凝胶型假体及生理盐水充注型假体于大鼠背侧皮下 ,D组为空白对照。术前测出血液中有机硅的浓度。 6个月后取出假体观察 ,进行假体包膜组织学检查及大鼠血液中术后有机硅的浓度测定。结果 :A、B、C 3组纤维包膜组织学特征无明显差异 ,B组纤维包膜中光镜下发现外渗的凝胶颗粒。术后与术前 4组大鼠间血液中有机硅的浓度比较 ,差异均无显著性。结论 :完整的硅凝胶假体、人为刺破外壳的硅凝胶假体与生理盐水充注型假体的组织学反应无明显差异 ,外渗的凝胶局限在纤维包膜的内层 ,也不会随血液或淋巴到机体其他部位。     

生长激素对糖尿病及其微血管病变的影响

目的 :探讨生长激素对糖尿病及其微血管病变的影响。方法 :采用放射免疫法测定 6 7例Ⅱ型糖尿病患者及 18例正常人的血清生长激素 (GH)及胰岛素 (INS)水平。结果 :糖尿病合并微血管病变组 ,空腹生长激素 (FGH)明显高于正常对照组 (P <0 .0 1)及糖尿病无微血管病变组 (P <0 .0 1)。糖尿病无微血管病变组的FGH与正常对照组的FGH相比未见明显差异 (P >0 .0 5 )。餐后 2h的生长激素 (PGH)在 3组之间均无显著差异 (P >0 .0 5 )。FGH与HbA1c、空腹血糖 (FBS)呈正相关 (r=0 .5 46 ,P <0 .0 1;r=0 .437,P <0 .0 1) ,与UAER呈正相关 (r=0 .486 ,P <0 .0 1) ;FGH与病程无相关 (r=0 .2 2 ,P >0 .0 5 ) ,与FINS无相关 (r =- 0 .0 2 9,P >0 .0 5 )。结论 :GH水平的增高 ,与糖代谢紊乱有关 ,参与了糖尿病微血管病变的发生。     

儿童肾病综合征患者血小板和血浆von Willebrand因子的变化

目的：观察儿童肾病综合征患者血小板计数和血浆von Willebrand因子的变化,方法,采用ELISA法检测58例NS患儿血浆vWF及血小板计数,并与正常对照组进行比较,结果：NS患儿vwF水平及血小板计数均明显高于正常对照组,两组有显著性差异（P＜0．01）,结论,血浆vWF与血小板计同步升高与NS患儿的高凝状态及血栓形成有一定的关系。     

人类巨细胞病毒感染对人胚肺细胞HOXB1,HOXB5,HOXB6及HOXB9基因表达的影响

目的 :研究人胚肺 (HEL)细胞HOXB1,HOXB5 ,HOXB6及HOXB9基因的表达状态及人类巨细胞病毒(HCMV)感染对上述基因表达的影响。方法 :采用半定量逆转录 -聚合酶链反应 (RT -PCR)技术。结果 :①HEL细胞表达HOXB5和HOXB6 ,但不表达HOXB1和HOXB9;②HCMV感染后 ,HEL细胞HOXB6基因的表达上调 ,且被HCMV诱导表达HOXB9T ,而HOXB5基因的表达无明显改变 ,HOXB1基因仍旧不表达 ;③全反式维甲酸 (ATRA)能上调HCMV感染后HOXB9基因的表达 ,但对HCMV感染晚期HOXB6基因的表达有抑制作用。结论 :HCMV能诱导HOXB6和HOXB9基因表达异常 ,这可能在先天性HCMV感染导致胚胎发育畸形中起重要作用     

Intradialytic systolic blood pressure variation can predict long-term mortality in patients on maintenance hemodialysis

International Urology and Nephrology - It is unclear which time-points of intradialytic blood pressure (BP) best predict prognosis. Thus, it is important to assess the association between different...     

The association between plant-based diet and erectile function in Chinese young healthy men: A population-based study

The study was designed and conducted to evaluate the plant-based diet status and its association with erectile function (EF) in Chinese young healthy men. From July 2018 to May 2020, 116 objectively proved physically and psychologically healthy men were selected. Clinical questionnaires, detailed physical examinations and blood tests were all assessed. An overall plant-based diet index (PDI) and a healthful plant-based diet index (hPDI) were developed from each participant to measure plant-based diet quantitatively. The EF was measured by both the International Index of Erectile Function-5 (IIEF-5) and the surrogated measures of endothelial function, peripheral levels of nitric oxide (NO) and E-selectin. In subjects, the mean PDI and hPDI were 53.8 ± 8.2 and 54.7 ± 8.9 respectively. Results of multivariate analysis showed that both PDI and hPDI were negatively associated with BMI (all p < .05), but had no relationships with NO, E-selectin, TT levels and IIEF-5 scores (all p > .05). In a subgroup of male population, Chinese young healthy men, the plant-based diet, measured by PDI and hPDI, was not related to erectile function. The population can adhere to plant-based diet to keep healthy without concerns of negative influence on erectile function.